Volume 23, Number 2—February 2017
Reoccurrence of Avian Influenza A(H5N2) Virus Clade 184.108.40.206 in Wild Birds, Alaska, USA, 2016
Historically, apparently effective geographic barriers (Bering and Chukchi Seas of the North Pacific Ocean) appeared to limit dissemination of Asian-origin, highly pathogenic avian influenza virus (HPAIV), such as influenza A(H5N1) virus A/goose/Guangdong/1/1996 (Gs/GD), between the Old and New Worlds (1). However, such barriers are incomplete; occasional spillovers of virus genes move from 1 gene pool to another (2). Asian-origin HPAIV H5N8 was identified in North America at the end of 2014 (3).
Novel HPAIVs H5N1, H5N2, and H5N8 emerged in late 2014 by reassortment with North American low pathogenicity avian influenza viruses (4). A novel reassortant H5N2 virus originating from Asian-origin H5N8 virus clade 220.127.116.11 and containing Eurasian polymerase basic 2, polymerase acidic, hemagglutinin, matrix, and nonstructural protein genes and North American lineage neuraminidase (NA), polymerase basic 1 (PB1), and nucleoprotein genes was identified on poultry farms in British Columbia, Canada, and in wild waterfowl in the northwestern United States. This virus subsequently predominated during influenza outbreaks in the United States in 2015.
During the boreal summer, birds from 6 continents (North America, South America, Asia, Africa, Australia, and Antarctica) fly to Alaska, USA, to breed. Thus, Alaska is a potentially major location for intercontinental virus transmission (1,2). Recent data provide direct evidence for viral dispersal through Beringia (5,6). Genetic evidence and waterfowl migratory patterns support the hypothesis that H5 virus clade 18.104.22.168 was introduced into North America through the Beringian Crucible by intercontinental associations with waterfowl (3). In addition, low pathogenicity avian influenza viruses were collected in Alaska before initial detection of H5 HPAIV clade 22.214.171.124, which contained genes that had recent common ancestry with reassortant H5N2 virus PB1, nucleoprotein, and NA (N2 subtype) genes and H5N1 virus PB1, polymerase acidic, NA (N1 subtype), and nonstructural protein genes of HPAIVs (7).
We report detection of an HPAIV H5N2 subtype from wild mallard sampled in Alaska during August 2016. Influenza A virus was detected in 48/188 dabbling duck samples collected during a live bird banding effort near Fairbanks, Alaska, during August 6–15, 2016. One sample of H5 virus from an adult mallard was identified as an HPAIV H5N2 on the basis of complete genome sequencing. We conducted comparative phylogenetic analysis of A/mallard/Alaska/AH0008887/2016(H5N2) virus, hereafter known as 8887/2016(H5N2) virus, to trace its origin and understand its genetic relationship to HPAIV H5N2 isolated in 2014–2015 (Technical Appendix[PDF - 2.18 MB - 8 pages]).
We considered 8887/2016(H5N2) virus an HPAIV on the basis of amino acid sequence at the hemagglutinin proteolytic cleavage site (PLRERRRKR/G), as shown for other Gs/GD HPAIV H5Nx subtypes in subclade 2.3.4 (http://www.offlu.net/fileadmin/home/en/resource-centre/pdf/Influenza_A_Cleavage_Sites.pdf). Homology BLAST searches showed that all genes had >99.2% nucleotide similarity with genes of H5N2 virus outbreak strains collected during late February–March 2015 (Technical Appendix[PDF - 2.18 MB - 8 pages] Table).
Phylogenetic analysis showed that the concatenated genome of 8887/2016(H5N2) virus formed a cluster with viruses from initial detections in the midwestern United States, including a snow goose in Missouri, a backyard poultry farm in Kansas, and a turkey farm in Minnesota (Figure). Our epidemiologic investigation data suggested that point-source introductions by indirect contact with wild waterfowl were the most probable source of infection for these backyard poultry in Kansas and a turkey farm in Minnesota (8). This genetic cluster was supported by a maximum-likelihood bootstrap value of 80 and a Bayesian posterior probability of 1.00.
The mean time to most recent common ancestry of viruses in this genetic cluster was estimated to be the end of January 2015 (mean time to most recent common ancestry January 27, 2015, 95% Bayesian credible interval January 11–February 10, 2015). Consistent clustering of 8887/2016(H5N2) virus with other H5N2 outbreak viruses in phylogenies for each gene suggests that the 8887/2016(H5N2) virus probably evolved through genetic drift from common ancestors of outbreak viruses in the absence of further reassortment (Technical Appendix[PDF - 2.18 MB - 8 pages] Figure 2). The mean rate of the nucleotide substitution obtained by Bayesian analysis was 6.064 × 10–3 (95% Bayesian credible interval 4.43–7.82 × 10–3) substitutions/site/year. In the root-to-tip regression plot of maximum-likelihood phylogeny, we found that 8887/2016(H5N2) virus fell below the regression line, which indicated sequences that are slightly less divergent than average of 2014–2015 H5N2 outbreak viruses (Technical Appendix[PDF - 2.18 MB - 8 pages] Figure 3).
The last reported detection during the influenza outbreak in the United States in 2015 was from a Canada goose in Michigan on June 17. There were 2 detections by PCR (3 assays, 2 gene targets, no virus recovered, no sequence obtained) from mallards in July (bird banding effort in Utah) and November (hunter harvest in Oregon) during surveillance in 2015–2016. Sequence of the HPAIV H5N2 from a wild mallard during surveillance in 2016–2017, evidence for continued evolution of this virus lineage, widespread detections of HPAIV H5N2 in healthy wild birds (9), and lack of pathobiological effects in experimentally infected waterfowl (10) collectively provide strong evidence for maintenance of HPAIV H5N2 in wild birds in North America. Detection of HPAIV in a mallard might imply the potential for dissemination of HPAIV H5N2 during the southward fall migration of waterfowl in 2016.
Dr. Lee is postdoctoral researcher at the US Department of Agriculture, Athens, GA. His research interests include molecular epidemiology and host–pathogen interactions for avian influenza viruses.
We thank Michael J. Petrula and David Sinnett for collecting samples; Kerrie Franzen, Meredith Grady, Andrew Hubble for providing technical assistance; the Washington State Animal Disease Diagnostic Laboratory for their participation in wild bird surveillance activities, and the originating and submitting institution (Kagoshima University, Kagoshima, Japan) for A/crane/Kagoshima/KU1/2014(H5N8) sequences (accession no. EPI169390] from the GISAID EpiFlu Database (http://platform.gisaid.org).
- Winker K, McCracken KG, Gibson DD, Pruett CL, Meier R, Huettmann F, et al. Movements of birds and avian influenza from Asia into Alaska. Emerg Infect Dis. 2007;13:547–52.
- Koehler AV, Pearce JM, Flint PL, Franson JC, Ip HS. Genetic evidence of intercontinental movement of avian influenza in a migratory bird: the northern pintail (Anas acuta). Mol Ecol. 2008;17:4754–62.
- Lee DH, Torchetti MK, Winker K, Ip HS, Song CS, Swayne DE. Intercontinental spread of Asian-origin H5N8 to North America through Beringia by migratory birds. J Virol. 2015;89:6521–4.
- Lee DH, Bahl J, Torchetti MK, Killian ML, Ip HS, DeLiberto TJ, et al. Highly pathogenic avian influenza viruses and generation of novel reassortants, United States, 2014–2015. Emerg Infect Dis. 2016;22:1283–5.
- Ramey AM, Reeves AB, Sonsthagen SA, TeSlaa JL, Nashold S, Donnelly T, et al. Dispersal of H9N2 influenza A viruses between East Asia and North America by wild birds. Virology. 2015;482:79–83.
- Lee DH, Park JK, Yuk SS, Erdene-Ochir TO, Kwon JH, Lee JB, et al. Complete genome sequence of a natural reassortant H9N2 avian influenza virus found in bean goose (Anser fabalis): direct evidence for virus exchange between Korea and China via wild birds. Infect Genet Evol. 2014;26:250–4.
- Ramey AM, Reeves AB, TeSlaa JL, Nashold S, Donnelly T, Bahl J, et al. Evidence for common ancestry among viruses isolated from wild birds in Beringia and highly pathogenic intercontinental reassortant H5N1 and H5N2 influenza A viruses. Infect Genet Evol. 2016;40:176–85.
- Animal and Plant Health Inspection Service, US Department of Agriculture. Epidemiologic and other analyses of HPAI-affected poultry flocks: September 9, 2015 Report [cited 2016 Oct 28]. https://www.aphis.usda.gov/animal_health/animal_dis_spec/poultry/downloads/Epidemiologic-Analysis-Sept-2015.pdf
- Bevins SN, Dusek RJ, White CL, Gidlewski T, Bodenstein B, Mansfield KG, et al. Widespread detection of highly pathogenic H5 influenza viruses in wild birds from the Pacific Flyway of the United States. Sci Rep. 2016;6:28980.
- Pantin-Jackwood MJ, Costa-Hurtado M, Shepherd E, DeJesus E, Smith D, Spackman E, et al. Pathogenicity and transmission of H5 and H7 highly pathogenic avian influenza viruses in mallards. J Virol. 2016;90:9967–82.