Inflammatory responses to dietary and surgical weight loss in male and female mice

Cameron Griffin,
Chelsea R. Hutch,
Simin Abrishami,
Daria Stelmak,
Leila Eter,
Ziru Li,
Eric Chang,
Devyani Agarwal,
Brian Zamarron,
Mita Varghese,
Perla Subbaiah,
Ormond A. MacDougald,
Darleen A. Sandoval and
Kanakadurga SingerEmail author View ORCID ID profile

Biology of Sex Differences201910:16

https://doi.org/10.1186/s13293-019-0229-7

Received: 3 January 2019
Accepted: 14 March 2019
Published: 3 April 2019

Abstract

Background

Weight loss by surgery or lifestyle changes is strongly recommended for obese individuals to improve metabolic health, but the underlying impairments that persist from a history of obesity remain unclear. Recent investigations demonstrate a persistent inflammatory state with weight loss and bariatric surgery, but the mechanism and impact are not fully understood. Additionally, these studies have not been performed in females although women are the majority of individuals undergoing weight loss interventions.

Methods

The goal of this study was to determine the sex differences in metabolically induced inflammation after dietary weight loss (WL) or bariatric surgery. Following a 60% high-fat diet (HFD) for 12 weeks, C57Bl/6j mice underwent either a dietary switch to normal chow for WL or vertical sleeve gastrectomy (VSG) and were evaluated 8 weeks after intervention. WL effects on myelopoiesis were further evaluated with bone marrow chimeras.

Results

Both sexes had a decrease in adiposity and total weight following WL or VSG intervention. With HFD, females had very little inflammation and no further increase with WL, but males had persistent inflammation even after WL despite metabolic improvement. Interestingly, after VSG, myeloid inflammation was increased in the livers of males and to a lesser extent in females.

Conclusions

These studies demonstrate that regardless of sex, it is critical to assess an individuals’ history of obesity rather than just rely on current weight status in medical decision-making. There are long-lasting effects on tissue inflammation in both sexes especially with surgical weight loss. Dietary change is overall most effective to improve meta-inflammation in obese males on its own or in combination with surgical weight loss.