CIENCIASMEDICASNEWS: Ensuring 100% Accuracy in Antibody Protein Sequencing

martes, 30 de abril de 2019

Ensuring 100% Accuracy in Antibody Protein Sequencing

	April 30, 2019
	Biochemistry
	The latest biochemistry news from AZoNetwork

Ensuring 100% Accuracy in Antibody Protein Sequencing
Antibody protein sequencing needs to accurately deduce every single amino acid present in the primary sequence. Even a single amino acid error in the primary sequence may have a damaging impact on the final antibody structure. Isoleucine and leucine which have the same molecular weight, which can mean that cannot be distinguished using standard mass spectrometry methods. W-ion based isoleucine leucine determination (WILD) is a new technique to determine these differences on a large scale with 100% accuracy.

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Consistency vs. Biocompatibility in Cell Cultures

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Analytical Techniques used in Human Biomonitoring

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	How to Reduce the Oxidation of Therapeutic Proteins Oxidants, such as reactive oxygen species (ROS), are known to induce damage to therapeutic proteins. Although cells and tissues usually have antioxidant systems to combat ROS and maintain the balance between formation and neutralization of ROS, in some cases this delicate balance may be perturbed, leading to physiological and molecular damage to proteins. In these cases, methods need to employed to reduce the likelihood of damage.

	Importance of Differentiating Between Leucine and Isoleucine As the name might suggest, isoleucine is, in fact, an isomer of leucine. This means that both amino acids have the same molecular formula but differ in structure. Importantly, the two isomers interact differently with other amino acids within mammalian cells. Hence, being able to identify and differentiate between leucine and isoleucine is essential.

	Using EC-MS to Study Neurotransmission Combining electrochemistry with mass spectrometry (EC-MS) provides a powerful method to determine the products and intermediates of electrochemical reactions. Apart from understanding the mechanism of various redox reactions, it can also be used for other applications, including the study of neurotransmission.

	What is Microdialysis? Microdialysis is a valuable tool in neuroscience that can be used to investigate and learn about complex brain functions through the collection of small-molecular-weight substances from the extracellular fluid or interstitial space.