Neuroscience News at NCTR
Model of Traumatic Brain Injury (TBI) to the Blood-Brain Barrier (BBB)
NCTR scientists — in collaboration with scientists from the University of Arkansas at Fayetteville and Universidad Autonoma de San Luis Potosi and Universidad Juarez Autonoma de Tabasco in Mexico — have developed an in vitro BBB model that simulates the BBB damage resulting from TBI observed in vivo. The model consists of primary rat-brain microvascular endothelial cells grown on a flexible substrate, which is then exposed to different percentages of deformation induced by biaxial stretch to model mild, moderate, and severe TBI. The results of the study show a stretch-dependent increase in cell death and apoptosis. The BBB regulates the passage of substances from the blood to the brain and vice versa. Disruption of this barrier, as can occur after severe head injuries (TBI), can induce neuroinflammation and cell death. This study demonstrates the utility of the in vitro BBB model in simulating TBI and supports the use of biaxial stretch as a valuable tool to study the mechanisms of TBI and potential therapies. A manuscript describing the newly developed model was published in Molecular Neurobiology
.
For more information, please contact Hector Rosas-Hernandez, Ph.D, or Syed Ali, Ph.D., Division of Neurotoxicology, FDA/NCTR
NCTR scientists — in collaboration with scientists from the University of Arkansas at Fayetteville and Universidad Autonoma de San Luis Potosi and Universidad Juarez Autonoma de Tabasco in Mexico — have developed an in vitro BBB model that simulates the BBB damage resulting from TBI observed in vivo. The model consists of primary rat-brain microvascular endothelial cells grown on a flexible substrate, which is then exposed to different percentages of deformation induced by biaxial stretch to model mild, moderate, and severe TBI. The results of the study show a stretch-dependent increase in cell death and apoptosis. The BBB regulates the passage of substances from the blood to the brain and vice versa. Disruption of this barrier, as can occur after severe head injuries (TBI), can induce neuroinflammation and cell death. This study demonstrates the utility of the in vitro BBB model in simulating TBI and supports the use of biaxial stretch as a valuable tool to study the mechanisms of TBI and potential therapies. A manuscript describing the newly developed model was published in Molecular Neurobiology
. For more information, please contact Hector Rosas-Hernandez, Ph.D, or Syed Ali, Ph.D., Division of Neurotoxicology, FDA/NCTR
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