CIENCIASMEDICASNEWS: Medical News | Medical Articles: Imaging Thick Samples of Large Model Organisms

viernes, 13 de septiembre de 2019

Medical News | Medical Articles: Imaging Thick Samples of Large Model Organisms

	September 13, 2019
	Fluorescence
	The latest fluorescence news from NewsMedical

Imaging Thick Samples of Large Model Organisms

Research projects transition from findings made at the single cell or subcellular level to more complex multicellular invertebrate and vertebrate model organisms to determine clinical relevance. In this article we discuss the challenges with laser scanning confocal microscopy (LSCM) and the solutions available.

			Plasmon-enhanced Fluorescence Spectroscopy Plasmon-enhanced fluorescence spectroscopy is a useful tool for imaging cells and other samples on a single-molecule level. This method poses several advantages over conventional fluorescence microscopy.

			Researchers illuminate mysterious aspect of mitochondrial metabolism using a tiny thermometer Armed with a tiny new thermometer probe that can quickly measure temperature inside of a cell, University of Illinois researchers have illuminated a mysterious aspect of metabolism: heat generation.

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	Ultrasensitive method for direct detection of circulating tumor cells in blood Tumor cells circulating in blood are markers for the early detection and prognosis of cancer. However, detection of these cells is challenging because of their scarcity. In the journal Angewandte Chemie, scientists have now introduced an ultrasensitive method for the direct detection of circulating tumor cells in blood samples. The gold standard for measuring has been with fluorescence, but it is too slow to see this short, high burst of heat.

	Sensing temperature inside cells using a fluorescent nano-thermometer The Rice lab of chemist Angel Martí revealed the technique in a Journal of Physical Chemistry B paper, describing how it modified a biocompatible molecular rotor known as boron dipyrromethene (BODIPY, for short) to reveal temperatures inside single cells. The molecule is ideally suited to the task. Its fluorescence lasts only a little while inside the cell, and the duration depends heavily on changes in both temperature and the viscosity of its environment.

	Red Fluorescent Protein Helps Explain Why Lung Tumors Turn Aggressive Lung cancer is the number 1 killer among cancers, especially when it is of the non-small cell type. Among these, adenocarcinomas are the most common and aggressive type. These tumors also show a strong tendency to show dedifferentiation, or loss of the features that characterize a mature lung cell.