CIENCIASMEDICASNEWS: Medical News | Medical Articles: Overcoming Challenges when Designing a Multicolor Flow Cytometry Panel

martes, 21 de mayo de 2019

Medical News | Medical Articles: Overcoming Challenges when Designing a Multicolor Flow Cytometry Panel

	May 21, 2019
	Flow Cytometry
	The latest flow cytometry news from News Medical

Overcoming Challengeswhen Designing a Multicolor Flow Cytometry Panel

Flow cytometry is a platform with the power to carry out the identification and characterization of multiple antigens at one time. At the same time, it must be noted that when more antigens and fluorochromes are employed, the experimental design becomes much more complex. To deal with this task, it is useful to consider the following five steps.

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			Flow Cytometry: Combinatorial Approaches to Cell Counting Flow cytometry is a versatile cell analysis technique that can be used to analyze multiple cell types. Combining flow cytometry with various other techniques can further increase its versatility and provide more detailed data.

			Flow Cytometry Cross-matching (FCXM) Flow cytometry cross-matching (FCXM) is used to compare HLA types present on APCs in the immune system prior to organ transplantation.

	Detecting Aneuploidy Using Flow Cytometry Aneuploidy is defined as a chromosomal aberration involving either an addition or deletion of chromosomes compared to the ‘normal' number of 46 chromosomes. Flow cytometry is a useful tool for detecting anueploidy.

	Novel technology set to play key role in the fight against anti-microbial resistance Professor Douglas Kell has developed technology using flow cytometry which detects and counts individual bacteria in a urine sample, and can determine which antibiotic is the most efficient at killing those particular bacteria.

	Researchers describe the impact of chronic hepatitis D on parts of the immune system Using flow cytometry, researchers have discovered that mucosal-associated invariant T (MAIT) cells, which are usually abundant in the liver, are deficient in patients with chronic hepatitis D.

	Researchers find presence of Aire gene in lymph nodes High sensitivity flow cytometry has revealed that 0.01% cells in the lymph nodes express Aire, a gene that regulates expression of tissue-restricted antigens in medullary thymic epithelial cells and has been associated with autoimmune diseases.

	Chinese FDA clears Cytek’s DxP Athena for clinical diagnostic use Thirteen-color flow cytometry will now be a routine part of clinical testing, as the Athena flow cytometer by Cytek Biosciences is approved for clinical diagnostic use in hospitals, laboratories and clinics across China.