CIENCIASMEDICASNEWS: Medical News | Medical Articles: Advances in Optical Methods for Systems Neuroscience

martes, 28 de mayo de 2019

Medical News | Medical Articles: Advances in Optical Methods for Systems Neuroscience

	May 28, 2019
	Fluorescence
	The latest fluorescence news from News Medical

Advances in Optical Methods for Systems Neuroscience

Many of the more difficult questions in neuroscience relate to complex processes such as perception of stimuli, formation of memories in learning, or alterations in behavior based on social contexts. One of the technologies that has been instrumental in this effort has been 2-photon or multiphoton fluorescence imaging.

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Developing Fluorescent Dyes for Use in Super-Resolution Microscopy

An essential tool in biological research is fluorescence imaging, which enables the visualization of proteins in live and fixed cells. Conventional techniques, such as confocal microscopy, produce image resolutions that are limited by the microscope and by the diffraction properties of light to around 200 nm.

Immunofluorescence Methods for IHC

Immunofluorescence, i.e. detection of antigens via fluorescence, can be used to observe several different antigens in one experiment. Immunofluorescence uses fluorochrome labels attached to antibodies, when the labels are excited by a specific wavelength of light they begin to emit light of a longer wavelength, allowing the presence and position of antigens in a sample to be determined.

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	E-FRET Microscopy for Live Cell Imaging E-FRET microscopy recently became a very popular intensity-based Fluorescence Resonance Energy Transfer (FRET) method for cell quantification. This technique reduces the photobleaching observed during the process of FRET.

	What is E-FRET Imaging? FRET, or fluorescent resonance energy transfer, is an imaging techniques used to visualize interactions between proteins in real time. E-FRET is a more powerful version of FRET which accounts for weaknesses in the original FRET method, allowing for more comparisons between laboratories and for more repeated measurements.

	New fluorescence-based method accurately determines strength of Lewis acids York University chemists have invented a new fluorescence-based method for accurately determining the strength of a range of Lewis acids, which could one day be used to help purify pharmaceutical drugs, improve industrial processes and explore next-generation technologies, according to a new chemistry study.

	New fluorescent sensor can detect migrating cancer cells A new fluorescent sensor developed by researchers from the University of Adelaide can detect migrating cancer cells and could be used to target medication to stop metastasis in aggressive cancers.

	Novel fluorescence imaging system helps surgeons remove small ovarian tumors Ovarian cancer is usually diagnosed only after it has reached an advanced stage, with many tumors spread throughout the abdomen.