domingo, 11 de noviembre de 2018

Oncogenic miR-663a is associated with cellular function and poor prognosis in renal cell carcinoma. - PubMed - NCBI

Oncogenic miR-663a is associated with cellular function and poor prognosis in renal cell carcinoma. - PubMed - NCBI



 2018 Sep;105:1155-1163. doi: 10.1016/j.biopha.2018.05.082. Epub 2018 Jun 21.

Oncogenic miR-663a is associated with cellular function and poor prognosis in renal cell carcinoma.

Zhou L1Pan X2Li Z3Chen P3Quan J2Lin C3Lai Y1Xu J4Xu W4Guan X4Li H4Gui Y5Lai Y6.

Abstract

BACKGROUND:

MicroRNA(miRNA) plays a key regulatory role in various stages of tumorigenesis, including cell growth, cell cycle control, apoptosis avoidance, tissue invasion, and metastasis. Several microRNAs are involved in the development of renal cell carcinoma (RCC) and the malignant transformation process. However, the effects of miR-663a on RCC have rarely been reported.

METHODS:

In the present study, the expression of miR-663a was examined in RCC using matched normal kidney tissues and four cell lines (786-O, Caki-1, ACHN and HK-2). MicroRNA mimics were transiently transfected into RCC cells and the effects of over expression on proliferation, migration, invasion, and apoptosis was observed. In addition, the relationship between miR-663a expression in 42 formalin-fixed paraffin-embedded (FFPE) clear cell renal carcinoma (ccRCC) samples and clinical pathological variables and overall survival was investigated. We evaluated the prognostic value of miR-663a expression in ccRCC by experimental results.

RESULTS:

The results showed that the expression of miR-663a was up-regulated in RCC cells and tissues and miR-663a was associated with proliferation, migration, invasion, and apoptosis of RCC. Cox proportional hazard regression analysis showed that a high expression of miR-663a patients had a significantly shorter overall survival in univariate and multivariate analysis. Kaplan-Meier survival curves showed that a high expression of miR-663a patients had a significantly shorter overall survival.

CONCLUSIONS:

These results indicate that miR-663a can be used as an independent marker for the poor prognosis of ccRCC, and may also play an important role as a tumor oncogene in the occurrence and development of RCC.

KEYWORDS:

Function; Prognosis; Renal cell carcinoma; miR-663a; microRNA

PMID:
 
30021352
 
DOI:
 
10.1016/j.biopha.2018.05.082

[Indexed for MEDLINE] 
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