Systemic translocation of Staphylococcus drives autoantibody production in HIV disease

Zhenwu Luo,
Min Li,
Yongxia Wu,
Zhefeng Meng,
Lisa Martin,
Lumin Zhang,
Elizabeth Ogunrinde,
Zejun Zhou,
Shenghui Qin,
Zhuang Wan,
Maria Anna Julia Westerink,
Stephanie Warth,
Hui Liu,
Ping Jin,
David Stroncek,
Quan-Zhen Li,
Ena Wang,
Xueling Wu,
Sonya L. Heath,
Zihai Li,
Alexander V. Alekseyenko and
Wei JiangEmail author View ORCID ID profile

Microbiome20197:25

https://doi.org/10.1186/s40168-019-0646-1

Received: 10 May 2018
Accepted: 5 February 2019
Published: 14 February 2019

Abstract

Background

Increased autoreactive antibodies have been reported in HIV disease; however, the mechanism accounting for autoantibody induction in HIV remains unknown.

Results

Herein, we show that seasonal influenza vaccination induces autoantibody production (e.g., IgG anti-nuclear antibody (ANA) and anti-double-stranded DNA antibody (anti-dsDNA)) in some viral-suppressed antiretroviral therapy (ART)-treated HIV+ subjects, but not in healthy controls. These autoantibodies were not derived from antigen-specific B cells but from activated “bystander” B cells analyzed by single-cell assay and by study of purified polyclonal ANAs from plasma. To explore the mechanism of autoantibody generation in HIV+ subjects, plasma level of microbial products, gene expression profile of B cells, and B cell receptor (BCR) repertoires were analyzed. We found that autoantibody production was associated with increased plasma level of microbial translocation; the patients with high autoantibodies had skewed B cell repertoires and upregulation of genes related to innate immune activation in response to microbial translocation. By analyzing circulating microbial 16S rDNA in plasma, the relative abundance of Staphylococcus was found to be associated with autoantibody production in HIV+ subjects. Finally, we found that injection of heat-killed Staphylococcus aureus promoted germinal center B cell responses and autoantibody production in mice, consistent with the notion that autoantibody production in HIV+ patients is triggered by microbial products.

Conclusions

Our results showed that translocation of Staphylococcus can promote B cell activation through enhancing germinal center response and induces autoantibody production. It uncovers a potential mechanism linking microbial translocation and autoimmunity in HIV+ disease and provides a strong rationale for targeting Staphylococcus to prevent autoantibody production.