domingo, 1 de abril de 2018

The Role of Next-Generation Sequencing in the Cytologic Diagnosis of Pancreatic Lesions. - PubMed - NCBI

The Role of Next-Generation Sequencing in the Cytologic Diagnosis of Pancreatic Lesions. - PubMed - NCBI



 2018 Apr;142(4):458-464. doi: 10.5858/arpa.2017-0215-RA.

The Role of Next-Generation Sequencing in the Cytologic Diagnosis of Pancreatic Lesions.

Abstract

CONTEXT:

- Integration of the analysis of genetic markers with endoscopic ultrasound-guided fine-needle aspiration and cytologic evaluation has increased the accuracy of the preoperative diagnosis of pancreatic lesions. The application of high-throughput gene panel analysis using next-generation sequencing platforms is now offering a great opportunity for further improvements.

OBJECTIVE:

- To review the application of next-generation sequencing to the preoperative diagnosis of pancreatic lesions.

DATA SOURCES:

- For data acquisition, a PubMed search using the terms next-generation sequencing, pancreas, pancreatic lesions, pancreatic tumors, and EUS-FNA was performed covering the years 2000-2017.

CONCLUSIONS:

- KRAS remains the gene most widely studied for preoperative single-gene tests. Next-generation sequencing reliably allows analysis of multiple gene markers starting from limited amounts of DNA. The study of multigene panels has become a very attractive option for the management and preoperative risk stratification of patients with pancreatic cancer.

PMID:
 
29565213
 
DOI:
 
10.5858/arpa.2017-0215-RA

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