- © 2016 by American Society of Clinical Oncology
Genomic Characterization of Primary Invasive Lobular Breast Cancer
- Christine Desmedt⇑,
- Gabriele Zoppoli,
- Gunes Gundem,
- Giancarlo Pruneri,
- Denis Larsimont,
- Marco Fornili,
- Debora Fumagalli,
- David Brown,
- Françoise Rothé,
- Delphine Vincent,
- Naima Kheddoumi,
- Ghizlane Rouas,
- Samira Majjaj,
- Sylvain Brohée,
- Peter Van Loo,
- Patrick Maisonneuve,
- Roberto Salgado,
- Thomas Van Brussel,
- Diether Lambrechts,
- Ron Bose,
- Otto Metzger,
- Christine Galant,
- François Bertucci,
- Martine Piccart-Gebhart,
- Giuseppe Viale,
- Elia Biganzoli,
- Peter J. Campbell and
- Christos Sotiriou
+Author Affiliations
- Corresponding author: Christine Desmedt, PhD, Breast Cancer Translational Research Laboratory, Institut Jules Bordet, 125 Boulevard de Waterloo, 1000 Brussels, Belgium; e-mail: christine.desmedt@bordet.be.
- Presented at the 37th Annual San Antonio Breast Cancer Symposium, San Antonio, TX, December 9-13, 2014.
- C.D., G.Z., G.G., P.J.C., and C.S. contributed equally to this work.
Abstract
Purpose Invasive lobular breast cancer (ILBC) is the second most common histologic subtype after invasive ductal breast cancer (IDBC). Despite clinical and pathologic differences, ILBC is still treated as IDBC. We aimed to identify genomic alterations in ILBC with potential clinical implications.
Methods From an initial 630 ILBC primary tumors, we interrogated oncogenic substitutions and insertions and deletions of 360 cancer genes and genome-wide copy number aberrations in 413 and 170 ILBC samples, respectively, and correlated those findings with clinicopathologic and outcome features.
Results Besides the high mutation frequency of CDH1 in 65% of tumors, alterations in one of the three key genes of the phosphatidylinositol 3-kinase pathway, PIK3CA,PTEN, and AKT1, were present in more than one-half of the cases. HER2 and HER3were mutated in 5.1% and 3.6% of the tumors, with most of these mutations having a proven role in activating the human epidermal growth factor receptor/ERBB pathway. Mutations in FOXA1 and ESR1 copy number gains were detected in 9% and 25% of the samples. All these alterations were more frequent in ILBC than in IDBC. The histologic diversity of ILBC was associated with specific alterations, such as enrichment for HER2 mutations in the mixed, nonclassic, and ESR1 gains in the solid subtype. Survival analyses revealed that chromosome 1q and 11p gains showed independent prognostic value in ILBC and that HER2 and AKT1 mutations were associated with increased risk of early relapse.
Conclusion This study demonstrates that we can now begin to individualize the treatment of ILBC, with HER2, HER3, and AKT1 mutations representing high-prevalence therapeutic targets and FOXA1 mutations and ESR1 gains deserving urgent dedicated clinical investigation, especially in the context of endocrine treatment.
Footnotes
- Supported by Susan G. Komen, Fondation MEDIC, Les Amis de Bordet, Fonds National de Recherche Scientifique, the Breast Cancer Research Foundation, the Wellcome Trust Clinical Research Training Fellowship Programs, the Italian Association for Leukemia and Lymphoma, and the Italian Association for Cancer Research.
- Authors’ disclosures of potential conflicts of interest are found in the article online at www.jco.org. Author contributions are found at the end of this article.
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