Misindentification of Mycobacterium kumamotonense as M. tuberculosis | CDC EID

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Volume 16, Number 7–July 2010
Letter
Misindentification of Mycobacterium kumamotonense as M. tuberculosis
Almudena Rodríguez-Aranda, María S. Jiménez, Jesús Yubero, Fernando Chaves, Rafael Rubio-García, Elia Palenque, María J. García, and M. Carmen Menéndez
Author affiliations: Hospital Universitario Doce de Octubre, Madrid, Spain (A. Rodríguez-Aranda, F. Chaves, R. Rubio Garcia, E. Palenque); Instituto de Salud Carlos III, Madrid (M.S. Jiménez); and Universidad Autónoma de Madrid, Madrid (J. Yubero, M.J. García, M.C. Menéndez)

Suggested citation for this article

To the Editor: Because of slow growth of mycobacteria, use of rapid tests to identify them is strongly recommended; rapid tests are widely used as an advanced diagnostic tool in clinical laboratories (1,2). These tests are particularly useful for diagnosing extrapulmonary mycobacterioses and identifying unusual mycobacteria as etiologic agents (3). Commercial probes are frequently used for rapid and specific identification of mycobacteria, especially Mycobacterium tuberculosis complex. However, cross-reactivity of DNA probes between mycobacterial species could result in incorrect diagnosis and treatment of patients (4,5). Misidentification could be a problem if a newly described species, such as M. kumamotonense (6), were an etiologic agent of a disease.

In July 2006, we obtained a fine-needle, puncture aspiration biopsy specimen from a cervical lymph node of a 30-year-old man at Doce de Octubre Hospital (Madrid, Spain). The patient was a recent immigrant from Paraguay and was HIV positive (C2 stage of infection). A biopsy specimen from a cervical lymph node showed necrotizing granulomatous lymphadenopathy. A computed tomographic scan showed cervico-thoraco-abdominal, multiple cervical, supraclavicular, axillar, paratracheal, and mediastinal lymphadenopathies. The patient had a CD4 cell count of 219 cells/mm3 and an HIV viral load of 197,181 copies/mL.

The aspiration sample was positive for acid-fast bacilli by fluorescent staining. The clinical isolate (designated 1369) obtained from the aspirate sample was grown in liquid media (MGIT Diagnostic Kit; Becton Dickinson Diagnostics, Sparks, MD, USA) and identified as M. tuberculosis complex by using the AccuProbe System (bioMérieux, Marcy l'Etoile, France).

A diagnosis of lymphoid tuberculosis was made, and the patient was treated with isoniazid, rifampin, ethambutol, and pyrazinamide. After 1 month, rifampin was withdrawn because of a cutaneous exanthem. Three months later, the clinical status of the patient had improved, fever had disappeared, and sizes of cervical and axillary lymph nodes had decreased. Treatment with tenofovir, emtricitabine, and lopinavir/ritonavir was started. Two weeks later, an immune reconstitution syndrome and adenopathies developed, but these resolved in 1 month.

Five months after treatment was started, susceptibility testing in a reference laboratory showed that isolate 1369 was M. kumamotonense. The isolate showed 100% identity with the 16S rRNA gene sequence of M. kumamotonense (GenBank accession no. AB239925). Results of PCR restriction analysis of heat shock protein 65 gene (7) (http://app.chuv.ch/prasite/index.html) were consistent with those for M. kumamotonense. The isolate was susceptible to ethambutol, rifampin, cycloserine, and ethionamide and resistant to isoniazid, streptomycin, pyrazinamide, and kanamycin.

Because of the improvement in the clinical status of the patient, treatment continued without modification for 18 months. At this time, his CD4 cell count was 488 cells/mm3 and his HIV viral load was <50 copies/mL. In July 2009, the patient was asymptomatic and had a CD4 cell count of 631 cells/mm3 and an HIV viral load <50 copies/mL.

To confirm misidentification of M. kumamotonense as a member of the M. tuberculosis complex, other commercial probes were tested. Isolate 1369 was also misidentified as M. tuberculosis complex by Inno-LIPA v2 (Innogenetics, Ghent, Belgium). The isolate was identified as Mycobacterium sp. by Geno-Type (Hain Lifescience, Nehren, Germany). The 3 commercial probes we used had different genome region specificities, all in the mycobacterial ribosomal operon. The AccuProbe System was specific for 16S rDNA, Inno-LIPA v2 was specific for internal transcribed spacer 1, and Geno-Type was specific for 23S rDNA. Only Geno-Type did not show cross-reactivity between M. tuberculosis complex and M. kumamotonense. The clinical isolate was identified as M. kumamotonense, a new, slow-growing mycobacterium that was first isolated from an immunocompetent patient in Japan (6). We showed that this species caused extrapulmonary disease in an HIV-positive patient.

Misidentification of M. kumamotonense as M. tuberculosis complex by commercial DNA probes has serious clinical implications. Once a patient is given a diagnosis of tuberculosis, he or she will be treated with specific drugs for a long period and be prone to adverse side effects. Furthermore, M. kumamotonense is resistant to many drugs used during typical treatment. After a diagnosis of tuberculosis, patient contacts need to be investigated to identify new cases. Emerging mycobacterial pathogens, such as M. kumamotonense, may also cause pulmonary and extrapulmonary infections that are also caused by other members of this genus and could be misidentified as M. tuberculosis.

This study was supported by the European Community Seventh Framework Programme (FP7-HEALTH-2007) under grant agreement no. 200999, and by the Spanish Network for Research in Infectious Diseases (RD06/008/0011).

References
1.Musial CE, Tice LS, Stockman L, Roberts GD. Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complex. J Clin Microbiol. 1988;26:2120–3.
2.Tortoli E, Nanetti A, Piersimoni C, Cichero P, Farina C, Mucignat G, et al. Performance assessment of new multiplex probe assay for identification of mycobacteria. J Clin Microbiol. 2001;39:1079–84. PubMed DOI
3.Jarzembowski JA, Young MB. Nontuberculous mycobacterial infections. Arch Pathol Lab Med. 2008;132:1333–41.
4.Butler WR, O'Connor SP, Yakrus MA, Gross WM. Cross-reactivity of genetic probe for detection of Mycobacterium tuberculosis with newly described species Mycobacterium celatum. J Clin Microbiol. 1994;32:536–8.
5.Lefmann M, Moter A, Schweickert B, Göbel UB. Misidentification of Mycobacterium leprae as Mycobacterium intracellulare by the COBAS AMPLICOR M. intracellulare test. J Clin Microbiol. 2005;43:1928–9. PubMed DOI
6.Masaki T, Ohkusu K, Hata H, Fujiwara N, Iihara H, Yamada-Noda M, et al. Mycobacterium kumamotonense sp. nov. recovered from clinical specimen and the first isolation report of Mycobacterium arupense in Japan: novel slowly growing, nonchromogenic clinical isolates related to Mycobacterium terrae complex. Microbiol Immunol. 2006;50:889–97.
7.Telenti A, Marchesi F, Balz M, Bally F, Böttger EC, Bodmer T. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol. 1993;31:175–8.

Suggested Citation for this Article
Rodríguez-Aranda A, Jiménez MS, Yubero J, Chaves F, Rubio-García R, Palenque E, et al. Misidentification of Mycobacterium kumamotonense as M. tuberculosis [letter]. Emerg Infect Dis [serial on the Internet]. 2010 Jul [date cited].
http://www.cdc.gov/EID/content/16/7/1178.htm

DOI: 10.3201/eid1607.091913

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Misindentification of Mycobacterium kumamotonense as M. tuberculosis | CDC EID