CIENCIASMEDICASNEWS: Beam Paths of the Movable Objective Microscope

viernes, 10 de mayo de 2019

Beam Paths of the Movable Objective Microscope

	May 10, 2019
	Life Science Microscopy
	The latest life science microscopy news from AZoNetwork

Beam Paths of the Movable Objective Microscope (MOM) Video
The field of in vivo, deep-tissue imaging is constantly advancing. In order to keep up with experimental breakthroughs, appropriate imaging systems should be designed with flexibility in mind. This video highlights the beam path options that help achieve this experimental flexibility.

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Imaging the Surface of Biological Tissue and Cells in the SEM

Scanning Electron Microscopes (SEM) are powerful magnification tools that utilize focused beams of electrons to obtain detailed topographical, morphological and compositional data. In the past samples have been limited to solid, inorganic samples small enough to fit inside a chamber under moderate vacuum pressure. In this article, we will look at equipment and techniques that increase the utility of modern SEM to provide biologists with more insight into cell function.

Analyzing Thin Films and Dopants at Sub-A° Equivalent Thickness

The rapid, quantitative and non-destructive determination of trace level dopants and elucidation of nanostructures is in high demand in nanotechnology and semiconductor research. Laboratory-based micro-XRF provides unique insight into these structures and facilitates flexible, accurate, quantitative analysis that provide several key advantages over existing techniques.

	Reflection Contrast Microscopy versus Brightfield Microscopy Brightfield microscopy has been widely used for hundreds of years, while reflection contrast microscopy is relatively new technology. As one of the earliest and simplest microscopy techniques, brightfield microscopy is easily accessible but lacks the depth achieved with reflection contrast microscopy.

	What is Single-Particle Cryo-Electron Microscopy? Single particle cryo-EM involves using electron microscopy to take up to millions of images of a biological sample using low-dose radiation. Cryo-EM has been in use since the 1970s, however, there has been renewed interest in the technique after scientists achieved near-atomic resolution, which is less than 4 å.

	What is E-FRET Imaging? FRET, or fluorescent resonance energy transfer, is an imaging techniques used to visualize interactions between proteins in real time. E-FRET is a more powerful version of FRET which accounts for weaknesses in the original FRET method, allowing for more comparisons between laboratories and for more repeated measurements.

	Scanning Electron Microscopy in Dental Studies In dental studies, microscopy is considered a versatile tool. While optical microscopy finds usage in day-to-day practices in dental clinics, the ability of electron microscopy to offer a large variety of surface information makes it perfect for use within a large variety of research subjects as well. The following examples offer added insights into how in-detail scanning electron microscopy (SEM) can be utilized in a variety of dental studies.