Volume 25, Number 6—June 2019
Research Letter
Suboptimal Handling of Piccolo Samples or Reagent Discs for Consideration in Ebola Response
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Jessica R. Spengler
, Stephen R. Welch, Sarah C. Genzer, JoAnn Coleman-McCray, Jessica R. Harmon, Stuart T. Nichol, and Christina F. Spiropoulou
, Stephen R. Welch, Sarah C. Genzer, JoAnn Coleman-McCray, Jessica R. Harmon, Stuart T. Nichol, and Christina F. SpiropoulouAbstract
Operating clinical analyzers within recommended parameters can be challenging during outbreak response. Using the Piccolo Xpress point-of-care blood chemistry analyzer on guinea pig blood, we found that values of many analytes are still readily comparable when samples and reagent discs are handled at various conditions outside of manufacturer’s recommendations.
Blood chemistry analyses are useful for guiding patient care. However, following manufacturer-recommended handling and storage conditions can be challenging in areas with underdeveloped infrastructure, as experienced in past and ongoing Ebola outbreak response (1). To investigate the utility of data from samples or reagent discs handled under suboptimal conditions, we evaluated 14 conditions outside of manufacturer’s recommendations by using Strain 13/N guinea pig blood and plasma samples. Animal procedures were approved by the Centers for Disease Control and Prevention Institutional Animal Care and Use Committee and conducted at an AAALAC-International–accredited facility.
Samples were run on the Abaxis Piccolo Xpress Chemistry Analyzer (https://www.abaxis.com; quality control with Abbot General Chemistry controls and verification sample, https://www.fishersci.com/shop/products/pic-lpd-pls-gen-chm-ct-2x6x1ml/07p0401a). This platform is a compact and portable Clinical Laboratory Improvement Amendments–approved automated point-of-care system for whole blood, serum, and plasma (2). This platform, together with the General Chemistry 13 reagent disc used here, is widely used in past and ongoing Ebola outbreak responses (3–6) and in laboratory research on viral pathogenesis, therapeutics, and vaccine efficacy (7–9). All samples were collected in the recommended lithium heparin (LiH) tubes, except as indicated.
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