Ahead of Print -Safety of Recombinant VSV–Ebola Virus Vaccine Vector in Pigs - Volume 21, Number 4—April 2015 - Emerging Infectious Disease journal - CDC
Volume 21, Number 4—April 2015
Safety of Recombinant VSV–Ebola Virus Vaccine Vector in Pigs
The current Ebola virus (EBOV) outbreak in West Africa has shown the need for an effective vaccine against this virus. As a result, clinical trials to test several vaccine candidates have been expedited (1) in hopes of contributing to containment of the outbreak. One of these vaccine candidates is based on a recombinant vesiculovirus vector species, vesicular stomatitis Indiana virus (here designated and more commonly known as VSV) expressing the EBOV strain Mayinga glycoprotein (here designated rVSV∆G/EBOVGP; formerly designated VSV∆G/ZEBOVGP) (2–4). This vaccine was highly efficacious in preexposure and postexposure studies in nonhuman primates after a single injection (5). In addition, the vaccine has been shown to be safe in simian HIV–infected rhesus macaques (6) and was not neurovirulent after intrathalamic inoculation into macaques (7).
However, because VSV is a World Organisation for Animal Health–listed pathogen (8), concerns might arise with regard to spillover of the vaccine vector to livestock when this vaccine is used on a larger scale in humans. To evaluate the safety of rVSV∆G/EBOVGP in a relevant livestock species, we inoculated pigs with this vaccine and compared clinical signs and virus replication with those of a recombinant wild-type VSV vector (rVSVwt) described previously (3).
Dr. de Wit is a research fellow in the Disease Modeling and Transmission Section of the Laboratory of Virology, Rocky Mountain Laboratories, Hamilton, Montana. Her research interests are pathogenesis and transmission of zoonotic emerging viruses.
We thank David Stallknecht, Lisa Kercher, and the Rocky Mountain Veterinary Branch for providing helpful advice.
This study was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health.
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Suggested citation for this article: de Wit E, Marzi A, Bushmaker T, Brining D, Scott D, Richt JA, et al. Safety of recombinant VSV–Ebola virus vaccine vector in pigs. Emerg Infect Dis. 2015 Apr [date cited]. http://dx.doi.org/10.3201/eid2104.142012
1Current affiliation: University of Colorado, Boulder, Colorado, USA.