domingo, 25 de mayo de 2014

Possible Misidentification of Mycobacterium yongonense - Volume 20, Number 6—June 2014 - Emerging Infectious Disease journal - CDC

Possible Misidentification of Mycobacterium yongonense - Volume 20, Number 6—June 2014 - Emerging Infectious Disease journal - CDC

link to Volume 20, Number 6—June 2014

Volume 20, Number 6—June 2014


Possible Misidentification of Mycobacterium yongonense

To the Editor: Tortoli et al. (1) reported pulmonary disease caused by M. yongonense strains isolated from patients in Italy; these strains were identified by sequencing the 16S rRNA, hsp65rpoB, and sodA genes and the internal transcribed spacer 1 (ITS1) region. The 16S rRNA gene sequence of these isolates showed 100% similarity with those of M. yongonense and M. marseillense. The isolates were more closely related to M. yongonense than to M. marseillense in terms of the hsp65 gene and ITS1 region; however, the rpoB gene sequence showed a higher degree of similarity to that of M. intracellulare (99.4%) than to that of M. marseillense (97.4%). The authors did not mention the similarity of the isolates with M. intracellulare in these sequences except for the rpoB gene. However, because these sequences showed high similarity to M. yongonense, a high degree of similarity to M. intracellulare could be inferred.
The initial description of M. yongonense highlighted its unique molecular character (2). The 16S rRNA and hsp65 genes and ITS1 region are closely related to those of M. intracellulare ATCC 13950T; however, the rpoB gene is closely related to that of M. parascrofulaceum ATCC BAA-614T(99.4%). No consensus guidelines are available for mycobacterial identification, but the rpoBgene has been used widely as a target gene; multilocus sequence analysis also has been used recently (3,4). Although the authors suggest that a variant of M. yongonense preceded the acquisition of the rpoB gene from M. parascrofulaceum by a lateral gene transfer event (3), the isolates described are more similar to M. intracellulare than to M. yongonense on the basis of therpoB gene sequence and multilocus sequence analysis. It is also possible that the isolates are aM. yongonense strain that preceded the acquisition of the rpoB gene but that are not the same as the initially described M. yongonense.
Sung Kuk HongComments to Author  and Eui-Chong Kim
Author affiliations: Seoul National University Hospital, Seoul, South Korea


  1. Tortoli EMariottini APierotti PSimonetti TMRossolini GMMycobacterium yongonense in pulmonary disease, Italy [letter]Emerg Infect Dis2013;19:19024DOIExternal Web Site IconPubMedExternal Web Site Icon
  2. Kim BJMath RKJeon COYu HKPark YGKook YHMycobacterium yongonense sp. nov., a slow-growing non-chromogenic species closely related to Mycobacterium intracellulare.Int J Syst Evol Microbiol2013;63:1929DOIExternal Web Site IconPubMedExternal Web Site Icon
  3. Kim BJHong SHKook YHKim BJMolecular evidence of lateral gene transfer in rpoBgene of Mycobacterium yongonense strains via multilocus sequence analysis. PLoS ONE.2013;8:e51846DOIExternal Web Site IconPubMedExternal Web Site Icon
  4. Macheras ERoux ALBastian SLeao SCPalaci MSivadon-Tardy VMultilocus sequence analysis and rpoB sequencing of Mycobacterium abscessus (sensu lato) strains. J Clin Microbiol2011;49:4919DOIExternal Web Site IconPubMedExternal Web Site Icon
Suggested citation for this article: Hong SK, Kim E-C. Possible misidentification of Mycobacterium yongonense [letter]. Emerg Infect Dis [Internet]. 2014 Jun [date cited]. Web Site Icon
DOI: 10.3201/eid2006.131508

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