Immunity - Development and Migration of Plasma Cells in the Mouse Lymph Node

Development and Migration of Plasma Cells in the Mouse Lymph Immunity, Volume 33, Issue 1, 118-127, 08 July 2010
Copyright 2010 Elsevier Inc. All rights reserved.
10.1016/j.immuni.2010.06.015

Referred to by: Plasma Cell Precursors: Long-Distance Tr...

Authors
David R. Fooksman, Tanja A. Schwickert, Gabriel D. Victora, Michael L. Dustin, Michel C. Nussenzweig, Dimitris SkokosSee AffiliationsHint: Rollover Authors and Affiliations Program in Molecular Pathogenesis and Department of Pathology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021 USA Laboratory of Molecular Immunology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021 USA Regeneron Pharmaceuticals, Tarrytown, NY 10591-6707, USA Corresponding author These authors contributed equally to this work

Highlights
•Pre-PCs migrate in random directions with long free-path lengths without chemokines
•Pre-PCs migrate by a processive slide, which facilitates a long free-path length
•Pre-PC motility is inversely correlated with differentiation and is cell autonomous
•PC retention in the medullary cords is Ptx sensitive

Summary
In this study, we imaged the differentiation and migratory behavior of nascent plasma cells (PCs) in mouse lymph nodes by intravital microscopy. Pre-PCs exhibited a unique migration pattern characterized by long, linear paths that were randomly oriented. Although chemotaxis via Gαi coupled-receptors has been implicated in PC migration, treatment with Pertussis toxin (Ptx), which ablates these signals, did not prevent movement of pre-PCs while it arrested other lymphocytes. In vitro, pre-PCs displayed processive amoeboid locomotion on surfaces coated with integrin ligand, whereas fully differentiated PCs moved slowly or were arrested. Both PC arrest and differentiation occurred in the medullary cords. Ptx treatment before PC differentiation blocked their accumulation in the medullary cords but pre-PCs still differentiated in other lymph node regions. Taken together, we suggest pre-PCs undergo a persistent random walk to find the medullary cords, where localized chemokines help retain these cells until they undergo differentiation and arrest in situ.
10.1016/j.immuni.2010.06.015


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Immunity - Development and Migration of Plasma Cells in the Mouse Lymph Node




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