CIENCIASMEDICASNEWS: Breakthrough observations made possible by label-free long-term 3D imaging of mitochondria

miércoles, 24 de octubre de 2018

Breakthrough observations made possible by label-free long-term 3D imaging of mitochondria

	October 24, 2018
	Life Science Microscopy
	The latest life science microscopy news from AZoNetwork

Label-Free Long-Term 3D Imaging of Mitochondria

Mitochondria are one of the most important cell organelles. They are organized in extremely dynamic networks and, in addition to supplying the cell with energy, they have an important role in apoptosis, metabolism, and degenerative diseases. Until now it was believed that, unless specifically stained, they were not visible in microscopy.

			Two-photon Microscopy Principles and Methodology Two-photon microscopy provides several advantages to confocal or fluorescence microscopy for imaging thick samples and removing out-of-focus light.

			Wide-field Fluorescence Microscopy Wide-field fluorescence microscopy is a widely applied imaging technique used to examine cells and investigate their internal structures.

	Minimizing Photobleaching in Fluorescence Microscopy Fluorescence microscopy is an imaging technique used to examine cells and their internal components. Specific cellular components can be labeled with fluorophores.

	Tomocube introduces new holotomography microscope for live cell imaging A novel optical microscope utilizing diffraction tomography to generate 3D holographic images of unlabelled live cells is now available from Tomocube. Called HT-1, the new microscope delivers quantitative nanoscale, real-time, dynamic images of individual living cells quickly and simply without any sample preparation.

	Holotomography in Cell Biology Holotomography uses lasers to measure the refractive index in all three dimensions. As the refractive index of different objects inside a cell varies, this method can be used as a contrast agent to visualise different components inside a cell.

	Chemically-Modified DNA Aptamers Improve the Power of Super-Resolution Fluorescence Microscopy In a revolutionary study, an LMU research team headed by Ralf Jungmann has shown that the application of chemically-modified DNA aptamers as protein markers enables one to improve the power of super-resolution fluorescence microscopy as an imaging instrument.