Projecting the evolutionary history of ST258.
BEAST analysis based on 1,425 core genome SNPs in 101 ST258 isolates with NJST258_1 reference genome, with the 215 kb region of recombination  masked, gives temporal context to the emergence of ST258, with key events and initial reports of KPC-producing K. pneumoniae in different countries plotted. Blue font indicates reports of KPC-producing K. pneumoniae, brown font is ST258. Green shading on the phylogeny shows lines of iterations of Bayesian analyses. The mean mutation rate of K. pneumoniae ST258 is 1.03 x 10−6 (95% HPD 8.09 x 10−7 to 1.24 x 10−6). The TMRCA for the ST258 clade is approximately 20 years ago, around 1995. The plot inset is a root-to-tip analysis of SNP accumulations for each isolate since the MRCA of ST258. The slope of the fit line is 4.66, which is close to the mutation rate calculated by BEAST ((1.03 x 10−6 substitutions per site per year) x (3.8 Mbp core genome size) = 3.9 SNPs per year).
PLoS One. 2015 Jul 21;10(7):e0133727. doi: 10.1371/journal.pone.0133727. eCollection 2015.
Genomic Analysis of the Emergence and Rapid Global Dissemination of the Clonal Group 258 Klebsiella pneumoniae Pandemic.
Bowers JR1, Kitchel B2, Driebe EM1, MacCannell DR2, Roe C1, Lemmer D1, de Man T2, Rasheed JK2, Engelthaler DM1, Keim P1, Limbago BM2.
- [PubMed - in process]