Volume 21, Number 4—April 2015
Safety of Recombinant VSV–Ebola Virus Vaccine Vector in Pigs
The current Ebola virus (EBOV) outbreak in West Africa has shown the need for an effective vaccine against this virus. As a result, clinical trials to test several vaccine candidates have been expedited (1) in hopes of contributing to containment of the outbreak. One of these vaccine candidates is based on a recombinant vesiculovirus vector species, vesicular stomatitis Indiana virus (here designated and more commonly known as VSV) expressing the EBOV strain Mayinga glycoprotein (here designated rVSV∆G/EBOVGP; formerly designated VSV∆G/ZEBOVGP) (2–4). This vaccine was highly efficacious in preexposure and postexposure studies in nonhuman primates after a single injection (5). In addition, the vaccine has been shown to be safe in simian HIV–infected rhesus macaques (6) and was not neurovirulent after intrathalamic inoculation into macaques (7).
However, because VSV is a World Organisation for Animal Health–listed pathogen (8), concerns might arise with regard to spillover of the vaccine vector to livestock when this vaccine is used on a larger scale in humans. To evaluate the safety of rVSV∆G/EBOVGP in a relevant livestock species, we inoculated pigs with this vaccine and compared clinical signs and virus replication with those of a recombinant wild-type VSV vector (rVSVwt) described previously (3).
All animal experiments were approved by the Institutional Animal Care and Use Committee of the Rocky Mountain Laboratories and performed following the guidelines of the Association for Assessment and Accreditation of Laboratory Animal Care, International. Experiments were performed by certified staff in an Association for Assessment and Accreditation of Laboratory Animal Care AAALAC–approved facility, following the guidelines and basic principles in the US Public Health Service Policy on Humane Care and Use of Laboratory Animals and the Guide for Care and Use of Laboratory Animals.
Four-week old pigs (Yorkshire cross) were obtained from the Washington State University College of Veterinary Medicine (Pullman, WA, USA). One group of 5 pigs and 1 group of 6 pigs were inoculated with rVSVwt and rVSV∆G/EBOVGP, respectively, as controls; 2 animals were mock inoculated with culture medium (Dulbecco modified Eagle medium). Animals were inoculated with 106 PFUs of either virus in a 100-μL volume, or an equal volume of Dulbecco modified Eagle medium by intradermal injection in the apex of the snout (9).
At regular intervals after inoculation, clinical examinations were performed to determine the health status of the animals and to collect nasal, throat, and rectal swab samples for virologic analysis; blood was collected to determine the humoral immune response. Three animals inoculated with rVSVwt and rVSV∆G/EBOVGP were euthanized at 3 days postinoculation (dpi) as per protocol; the remaining animals were euthanized at 21 dpi.
Inoculation of pigs with rVSVwt and rVSV∆G/EBOVGP did not result in obvious signs of disease (Table), changes in body temperature, or a decrease in weight gain compared with mock-inoculated controls. A nose lesion developed at 4 dpi at the injection site in 1 animal inoculated with rVSVwt, but this lesion healed by 9 dpi. Swab specimens collected from the lesion site on 5, 6, 7, 8, and 10 dpi were negative by virus titration. Nose, throat, and rectal swab specimens were collected at 1, 3, 6, 10, 14, and 21 dpi; a nose swab specimen collected at 3 dpi from a pig inoculated with rVSV∆G/EBOVGP was the only specimen in which virus could be detected (virus titer 100.83 50% tissue culture infectious dose [TCID50/mL]) (Table).
Three animals in each group were euthanized at 3 dpi. Tissue samples from lip, tongue, snout, footpad, coronary band, interdigital skin, tonsil, oronasopharynx, inguinal lymph node, axillary lymph node, cervical lymph node, mesenteric lymph node, bronchial lymph node, nasal mucosa, trachea, bronchus, lungs, heart, liver, spleen, kidney, adrenal gland, pancreas, jejunum, colon, urinary bladder, cervical spinal cord, frontal brain, cerebellum, and brain stem of these animals were collected for virus titration and histologic analysis.
In 2 of 3 animals inoculated with rVSVwt, virus was detected in the snout (virus titers 102.3 and 102.6 TCID50/g), but virus was not detected in any of the other tissues collected at 3 dpi. In 1 of the pigs inoculated with rVSV∆G/EBOVGP, virus was detected in the snout (virus titer 102.5 TCID50/g) and in another pig in an inguinal lymph node (virus titer 105.1 TCID50/g). This high titer in the inguinal lymph node might have been the result of a change in cell tropism caused by use of EBOVGP rather than VSV glycoprotein (VSV-G). Histologic analysis did not identify lesions consistent with VSV infection in the rVSVwt– or rVSV∆G/EBOVGP–inoculated animals. At the end of the experiment at 21 dpi, the same tissues were collected for virologic and histologic analysis; virus could no longer be detected in any of the tissues derived from the inoculated animals, and no histologic lesions were present.
Serum samples collected at 21 dpi were analyzed for IgG against VSV-G or EBOVGP by using an IgG ELISA and secreted forms of these glycoproteins as antigens (10,11), respectively. In the VSV-G ELISA, pigs inoculated with rVSVwt showed an antibody response to VSV-G at 21 dpi (titers 1:400 and 1:1600); animals inoculated with rVSV∆G/EBOVGP did not show seroconversion to VSV-G. In an ELISA specific for the EBOVGP, pigs inoculated with rVSV∆G/EBOVGP showed a robust antibody response (titers 1:800, 1:1,600, and 1:3,200 by 21 dpi), but animals inoculated with rVSVwt did not show seroconversion to EBOVGP.
Our data indicate that, although rVSV∆G/EBOVGP can replicate in pigs, this vaccine virus does not result in overt clinical disease, and virus shedding is minimal. Because a high dose of the vaccine was directly injected intradermally into the snouts of the animals in this study and yet did not cause disease, it is unlikely that vaccination of humans with the rVSV∆G/EBOVGP vector would result in a productive infection with clinical disease in domestic pigs during a spillover event. Moreover, even if this spillover were to occur, the near absence of virus shedding in the rVSV∆G/EBOVGP–infected animals suggests that spillover would not result in maintenance of rVSV∆G/EBOVGP within a pig herd. This study provides data to support the safety of the live-attenuated VSV∆G/EBOVGP vaccine in a relevant livestock species. Should exposure/infection of pigs occur during a vaccination trial in humans, it is highly unlikely that signs of disease would develop in pigs or that the vaccine virus would be disseminated by interspecies or intraspecies transmission.
Dr. de Wit is a research fellow in the Disease Modeling and Transmission Section of the Laboratory of Virology, Rocky Mountain Laboratories, Hamilton, Montana. Her research interests are pathogenesis and transmission of zoonotic emerging viruses.
We thank David Stallknecht, Lisa Kercher, and the Rocky Mountain Veterinary Branch for providing helpful advice.
This study was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health.
- Arie S. Trial of Ebola virus vaccine is due to start next week. BMJ. 2014;349:g5562 . DOIPubMed
- Garbutt M, Liebscher R, Wahl-Jensen V, Jones S, Moller P, Wagner R, Properties of replication-competent vesicular stomatitis virus vectors expressing glycoproteins of filoviruses and arenaviruses. J Virol. 2004;78:5458–65. DOIPubMed
- Lawson ND, Stillman EA, Whitt MA, Rose JK. Recombinant vesicular stomatitis viruses from DNA. Proc Natl Acad Sci U S A. 1995;92:4477–81.DOIPubMed
- Jones SM, Feldmann H, Stroher U, Geisbert JB, Fernando L, Grolla A, Live attenuated recombinant vaccine protects nonhuman primates against Ebola and Marburg viruses. Nat Med. 2005;11:786–90. DOIPubMed
- Geisbert TW, Feldmann H. Recombinant vesicular stomatitis virus–based vaccines against Ebola and Marburg virus infections. J Infect Dis.2011;204(Suppl 3):S1075–81. DOIPubMed
- Geisbert TW, Daddario-Dicaprio KM, Lewis MG, Geisbert JB, Grolla A, Leung A, Vesicular stomatitis virus–based Ebola vaccine is well-tolerated and protects immunocompromised nonhuman primates. PLoS Pathog. 2008;4:e1000225. DOIPubMed
- Mire CE, Miller AD, Carville A, Westmoreland SV, Geisbert JB, Mansfield KG, Recombinant vesicular stomatitis virus vaccine vectors expressing filovirus glycoproteins lack neurovirulence in nonhuman promates. PLoS Negl Trop Dis. 2012;6:e1567. Epub 2012 Mar 20.
- World Organisation for Animal Health (OIE). OIE-listed diseases, infections and infestations in force in 2014 [cited 2015 Jan 20].http://www.oie.int/en/animal-health-in-the-world/oie-listed-diseases-2014/
- Stallknecht DE, Greer JB, Murphy MD, Mead DG, Howerth EW. Effect of strain and serotype of vesicular stomatitis virus on viral shedding, vesicular lesion development, and contact transmission in pigs. Am J Vet Res. 2004;65:1233–9. DOIPubMed
- Marzi A, Ebihara H, Callison J, Groseth A, Williams KJ, Geisbert TW, Vesicular stomatitis virus–based Ebola vaccines with improved cross-protective efficacy. J Infect Dis. 2011;204(Suppl 3):S1066–74. DOIPubMed
- Nakayama E, Yokoyama A, Miyamoto H, Igarashi M, Kishida N, Matsuno K, Enzyme-linked immunosorbent assay for the detection of filovirus species-specific antibodies. Clin Vaccine Immunol. 2010;17:1723–8. DOIPubMed
Suggested citation for this article: de Wit E, Marzi A, Bushmaker T, Brining D, Scott D, Richt JA, et al. Safety of recombinant VSV–Ebola virus vaccine vector in pigs. Emerg Infect Dis. 2015 Apr [date cited]. http://dx.doi.org/10.3201/eid2104.142012
1Current affiliation: University of Colorado, Boulder, Colorado, USA.
No hay comentarios:
Publicar un comentario