Travel-related Schistosomiasis Acquired in Laos | CDC EID

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Volume 15, Number 11–November 2009
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Travel-related Schistosomiasis Acquired in Laos
Eyal Leshem, Eyal Meltzer, Esther Marva, and Eli Schwartz
Author affiliations: Chaim Sheba Medical Center, Tel Hashomer, Israel (E. Leshem, E. Meltzer, E. Schwartz); Tel Aviv University Sackler School of Medicine, Ramat Aviv, Israel (E. Leshem, E. Meltzer, E. Schwartz); and Ministry of Health, Jerusalem, Israel (E. Marva)

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Abstract
Twelve Israeli travelers acquired schistosomiasis in Laos during 2002–2008, and 7 of them had acute schistosomiasis. The patients were probably exposed to Schistosoma mekongi in southern Laos, an area known to be endemic for schistosomiasis. Four possibly were infected in northern Laos, where reports of schistosomiasis are rare.

Figure

Figure. Map of Laos. The area in which Schistosoma mekongi is known to be endemic is highlighted in light blue...

Schistosomiasis is a widely distributed intravascular trematode infection. Estimates indicate that >200 million people are infected with schistosomiasis, mainly in Africa. In Asia, 3 Schistosoma species cause human infection: S. japonicum, S. malayensis, and S. mekongi. S. mekongi endemicity is thought to be limited to a 200-km area of the Mekong River Basin, stretching from the southern tip of Laos to Cambodia (Figure) (1). However, this parasite's intermediary host, freshwater snails (Neotricula aperta), has recently been found to be more widespread and to be advancing northwards (1,2). Attwood has suggested that S. mekongi may extend as far north as Khammouane Province in southern Laos (Figure) (1,3).

Acute schistosomiasis is a transient hypersensitivity reaction associated with tissue migration of Schistosoma spp. larvae in nonimmune persons. This syndrome is characterized by fever, cough, fatigue, myalgia, urticaria, and gastrointestinal complaints. Although acute schistosmiasis caused by S. japonicum was extensively studied long ago (4), we found no reports of acute schistosmiasis caused by S. mekongi. Moreover, current literature states that acute schistosmiasis has never been described as a feature of S. mekongi infection (5).

The Study
The study was conducted at the Center for Geographic Medicine at Sheba Medical Center and was approved by the institutional review board. Travel-related schistosomiasis was defined as a case of schistosomiasis confirmed by serology or ova detection in a traveler who had been exposed to freshwater in Laos. Travelers were thoroughly questioned regarding freshwater exposures during the index trip and any previous trips to schistosomiasis-endemic areas.

Serologic diagnosis conducted at the Israel Ministry of Health (MOH) Central Laboratories in Jerusalem was based on the soluble egg antigen ELISA test (IVD Research Inc., Carlsbad, CA, USA), a nonspecies-specific method. Consequently, most samples (11/12) were sent to the US Centers for Disease Control and Prevention (CDC) for species-specific serologic assays (Falcon Assay Screening Test ELISA [FAST-ELISA], CDC, Atlanta, GA, USA) (6). This method is used for S. japonicum serodiagnosis; specific serology for S. mekongi is unavailable.

Stool specimens were tested for the presence of Schistosoma spp. ova (merthiolate–iodine-formaldehyde technique) at the Israel MOH and Clalit Health Services laboratories. Fisher exact test was used for categoric data and the Student t test for continuous data. Statistical significance was set at p<0.05.

During 2002–2008, 12 patients (5 male, 7 female [2 children]) had travel-related schistosomiasis acquired in Laos (Table 1). No freshwater exposures in Schistosoma-endemic areas in Asia (excluding the index trip to Laos) were reported by patients (Table 1).

Mean patient age was 24 years (range, 6–42 years). Seven patients were exposed to freshwater in both southern and northern Laos (4,000 Islands and the town of Vang Vieng, respectively); 1 patient was exposed only in southern Laos (Figure). Notably, 4 patients reported freshwater exposure exclusively in northern Laos (Vang Vieng). Three of the 4 reported no travel in southern Laos; 1 patient (Table 1, patient 10) visited southern Laos but was not exposed to freshwater. Exposure occurred during the months of February–April for 9 of the 12 patients.

Seven patients had a diagnosis of acute schistosmiasis. Fever (86%), headache (86%), urticarial rash (71%), and cough (71%) were the most prevalent acute schistosmiasis symptoms (Table 2). Four patients reported chronic gastrointestinal symptoms (abdominal pain and discomfort, diarrhea or loose stools). One patient described a pruritic papular rash that appeared hours after exposure and resolved within a few days (suspected cercarial dermatitis). The patient was asymptomatic upon evaluation at our clinic (Table 1, patient 12).

Diagnosis was made by positive serology in all 12 patients. Eleven serum samples were sent to CDC for speciation; 9 patients had positive immunoblots for S. japonicum (Table 1). One patient had a positive immunoblot for S. haematobium; this result was judged to be a cross-reaction because the patient had never visited S. haematobium–endemic areas.

S. mekongi/japonicum ova were detected in stool samples of 1 of 7 patients who submitted such samples for ova detection (Table 1). Issues of technical proficiency and expertise precluded a definite conclusion regarding speciation according to egg size. Laboratory findings in 5 patients with acute schistosmiasis were significant for marked eosinophilia (Table 2).

All infected patients were treated with praziquantel at >12 weeks postexposure to avoid treatment failure (8). Of the acute schistosmiasis patients, 3 of the 7 required corticosteroid treatment during the acute illness.

Conclusions
Acute schistosmiasis, which is considered to be a hypersensitivity reaction that usually develops a few weeks after Schistosoma infection, is best studied in nonimmune travelers rather than in continuously exposed local populations. We report 7 cases of acute schistosmiasis presumably caused by S. mekongi infection acquired in Laos. Acute schistosmiasis is reportedly not a species-specific phenomenon but may develop after infection with any Schistosoma spp. (8), a view strengthened by this report.

Symptoms of acute schistosmiasis caused by S. mekongi, although a small number of cases, appear similar to symptoms of acute schistosmiasis caused by S. mansoni or S. haematobium (7) (Table 2). The only symptom significantly more prevalent in acute schistosmiasis caused by S. mekongi was headache.

Most Schistosoma infections in travelers are acquired in Africa (8,9). In our clinic, travel-related schistosomiasis acquired outside Africa was diagnosed only in travelers exposed in Laos (8). This exposure is probably due to the popularity of water-related adventure activities among travelers to Laos.

S. mekongi–endemic areas in Laos have presumably included only the southern reaches of the Mekong River (Figure) (1,2,5). However, this assumption may reflect a serendipitous effect because schistosomiasis in Laos was first diagnosed in immigrants originating from this region. These early schistosomiasis cases led early epidemiologic surveys to the region of Khong, where most subsequent studies were performed (5,10). Since these epidemiologic surveys were conducted, S. mekongi infections acquired in northern Laos have been described only anecdotally (11–13).

In this report, we describe 4 patients with schistosomiasis apparently acquired in northern Laos (Figure) after exposure to freshwater exclusively in Vang Vieng; that is, they reported no other freshwater exposure during their visit to Laos. However, because of lack of species-specific serology and the inability to find Schistosoma ova in these patients' stool samples, we can not determine which Schistosoma spp. caused their infection.

Most of these patients were infected during February–April, Mekong's early low-water period, indicating a seasonal infection pattern similar to that of local populations (5). The increased risk of schistosomiasis during the late dry season should be conveyed to travelers during pretravel consultations.

Our observation of Schistosoma infection in the 4 travelers exposed exclusively in Vang Vieng has several limitations. First, the diagnosis was based on positive serology and not on ova detection. Cross-reactivity of nonhuman Schistosoma spp. with S. japonicum in serologic studies (e.g., S. sinensium or S. ovuncatum) could have caused seropositivity in our patients. Second, these 4 travelers (Table 1, patients 9–12) have visited other areas in Asia known to be Schistosoma endemic (China) or suspected to be (Vietnam, Nepal). Although travelers were thoroughly questioned regarding possible freshwater exposures, they may not have recalled minor exposures. Finally, we found no published malacologic surveys of the Vang Vieng area, and most experts regard this area as free from N. aperta, the intermediate host of S. mekongi.

In other world regions (e.g., Lake Malawi in Africa), Schistosoma-infected travelers have served as sentinels alerting local authorities to previously unsuspected foci of transmission (14). The cases of schistosomiasis in travelers thought to be exposed only in northern Laos, an area where dam building may have changed local conditions, mandates a systematic revaluation of S. mekongi distribution in Laos.

Acknowledgments
We thank Patricia Wilkins and Shulamit Loewenthal for their valuable assistance.

Dr Leshem is a fellow in infectious diseases at The Chaim Sheba Medical Center. His interests include travel and tropical medicine.

References
Attwood SW. Schistosomiasis in the Mekong region: epidemiology and phylogeography. Adv Parasitol. 2001;50:87–152. PubMed DOI
Attwood SW, Fatih FA, Campbell I, Upatham ES. The distribution of Mekong schistosomiasis, past and future: preliminary indications from an analysis of genetic variation in the intermediate host. Parasitol Int. 2008;57:256–70. PubMed DOI
Attwood SW, Fatih FA, Upatham ES. DNA-sequence variation among Schistosoma mekongi populations and related taxa: phylogeography and the current distribution of Asian schistosomiasis. PLoS Negl Trop Dis. 2008;2:e200. PubMed DOI
Billings FT, Winkenwerder WN, Hunninen AV. Studies on acute schistosomiasis japonica in the Philippine islands. Johns Hopkins Hosp Bull. 1946;78:21–56.
Ohmae H, Sinuon M, Kirinoki M, Matsumoto J, Chigusa Y, Socheat D, et al. Schistosomiasis mekongi: from discovery to control. Parasitol Int. 2004;53:135–42. PubMed DOI
Tsang VC, Wilkins PP. Immunodiagnosis of schistosomiasis. Immunol Invest. 1997;26:175–88. PubMed DOI
Leshem E, Maor Y, Meltzer E, Assous M, Schwartz E. Acute schistosomiasis outbreak: clinical features and economic impact. Clin Infect Dis. 2008;47:1499–506. PubMed DOI
Meltzer E, Artom G, Marva E, Assous MV, Rahav G, Schwartzt E. Schistosomiasis among travelers: new aspects of an old disease. Emerg Infect Dis. 2006;12:1696–700.
Nicolls DJ, Weld LH, Schwartz E, Reed C, von Sonnenburg F, Freedman DO, et al. Characteristics of schistosomiasis in travelers reported to the GeoSentinel Surveillance Network 1997–2008. Am J Trop Med Hyg. 2008;79:729–34.
Doumenge JD. Democratic Kampuchea, Lao People's Democratic Republic, Thailand, Malasia, India. In: Doumenge JD, Mott KE, Cheung C, Villenave D, Chapuis O, Perrin MF, et al., editors. Atlas of the global distribution of schistosomiasis. Bordeaux (France): Presses Universitaire de Bordeaux, 1987; 375-381.
Iijima T, Garcia RG, Lo C-T. Studies on schistosomiasis in the Mekong Basin III. Prevalence of Schistosoma infection among the inhabitants. Kisechugaku Zasshi. 1973;22:338–46.
Wittes R, MacLean JD, Law C, Lough JO. Three cases of schistosomiasis mekongi from northern Laos. Am J Trop Med Hyg. 1984;33:1159–65.
Lorette G, Jaafar MR, Grojean MF, Duong T. Schistosomiasis mekongi diagnosed by rectal biopsy. Br Med J (Clin Res Ed). 1983;286:2012–3. PubMed DOI
Cetron MS, Chitsulo L, Sullivan JJ, Pilcher J, Wilson M, Noh J, et al. Schistosomiasis in Lake Malawi. Lancet. 1996;348:1274–8. PubMed DOI
Figure
Figure. Map of Laos. The area in which Schistosoma mekongi is known to be endemic is highlighted in light blue...

Tables
Table 1. Demographic, epidemiologic, and clinical characteristics of travelers with schistosomiasis acquired in Laos
Table 2. Clinical characteristics of patients with acute schistosomiasis acquired in Laos compared with those of case-patients from Tanzania

Suggested Citation for this Article
Leshem E, Meltzer E, Marva E, Schwartz E. Travel-related schistosomiasis acquired in Laos. Emerg Infect Dis [serial on the Internet]. 2009 Nov [date cited]. Available from http://www.cdc.gov/EID/content/15/11/1823.htm

DOI: 10.3201/eid1511.090611

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