Cancer Res Treat. 2018 May 9. doi: 10.4143/crt.2018.012. [Epub ahead of print]
Investigating the Feasibility of Targeted Next-Generation Sequencing to Guide the Treatment of Head and Neck Squamous Cell Carcinoma.
Lim SM1, Cho SH2, Hwang IG3, Choi JW4, Chang H5, Ahn MJ6, Park KU7, Kim JW8, Ko YH9, Ahn HK10, Cho BC11, Nam BH12, Chun SH13, Hong JH14, Kwon JH15, Choi JG16, Kang EJ17, Yun T18, Lee KW8, Kim JH1, Kim JS19, Lee HW20, Kim MK21, Jung D22, Kim JE23, Keam B24, Yun HJ25, Kim S26, Kim HR11.
Head and neck squamous cell carcinoma (HNSCC) is a deadly disease in which precision medicine needs to be incorporated. We aimed to implement next-generation sequencing (NGS) in determining actionable targets to guide appropriate molecular targeted therapy in HNSCC patients.
MATERIALS AND METHODS:
Ninety-three tumors and matched blood samples underwent targeted sequencing of 244 genes using the Illumina HiSeq 2500 platform with an average depth of coverage of greater than 1,000×. Clinicopathological data from patients were obtained from 17 centers in Korea, and were analyzed in correlation with NGS data.
Ninety-two of the 93 tumors were amenable to data analysis. TP53 was the most common mutation, occurring in 47 (51%) patients, followed by CDKN2A (n=23, 25%), CCND1 (n=22, 24%) and PIK3CA (n=19, 21%). The total mutational burden was similar between human papillomavirus (HPV)-negative vs. positive tumors, although TP53, CDKN2A and CCND1 gene alterations occurred more frequently in HPV-negative tumors. HPV-positive tumors were significantly associated with immune signature-related genes compared to HPV-negative tumors. Mutations of NOTCH1 (p=0.027), CDKN2A (p<0.001) and TP53 (p=0.038) were significantly associated with poorer overall survival. FAT1 mutations were highly enriched in cisplatin responders, and potentially targetable alterations such as PIK3CA E545K and CDKN2A R58X were noted in 14 (15%) patients.
We found several targetable genetic alterations, and our findings suggest that implementation of precision medicine in HNSCC is feasible. The predictive value of each targetable alteration should be assessed in a future umbrella trial using matched molecular targeted agents.
Biomarkers; Clinical trial; Molecular Targeted Therapy; Next-generation sequencing; Squamous cell carcinoma of the head and neck
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