Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK | BMC Research Notes | Full Text

Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK | BMC Research Notes | Full Text

BMC Research Notes

Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK

• Kimitoshi Denda, 
• Kanako Ida, 
• Masataka Tanno, 
• Kanako Nakao-Wakabayashi, 
• Masayuki Komada & 
• Nobuhiro Hayashi 

BMC Research Notes volume 12, Article number: 785 (2019) Cite this article

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Abstract

Objective

NRK is a unique X chromosome-linked protein kinase expressed predominantly in placenta. The gene knockout causes placental overgrowth and delayed labor of Nrk-null fetuses from dams in mouse. To clarify unknown mechanisms behind the Nrk-null phenotypes, protein expression profiles were analyzed in the Nrk-null placenta using a high-performance two-dimensional electrophoresis methodology.

Results

Among around 1800 spots detected, we characterized a dozen protein spots whose expression levels were significantly altered in the Nrk-null placenta compared to wild-type. Analyzing these data sets is expected to reflect the difference physiologically in the presence or absence of NRK, facilitating the development of therapeutic strategies.