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Emergence and Spread of Cephalosporin-Resistant Neisseria gonorrhoeae with Mosaic penA Alleles, South Korea, 2012–2017 - Volume 25, Number 3—March 2019 - Emerging Infectious Diseases journal - CDC

Emergence and Spread of Cephalosporin-Resistant <em>Neisseria gonorrhoeae</em> with Mosaic <em>penA</em> Alleles, South Korea, 2012–2017 - Volume 25, Number 3—March 2019 - Emerging Infectious Diseases journal - CDC

Volume 25, Number 3—March 2019


Emergence and Spread of Cephalosporin-Resistant Neisseria gonorrhoeae with Mosaic penA Alleles, South Korea, 2012–2017

Hyukmin Lee, Young Hee Suh, Sunhwa Lee, Yong-Kyun Kim, Mi-Soon Han, Hye Gyung Bae, Magnus Unemo1Comments to Author , and Kyungwon Lee1Comments to Author 
Author affiliations: Yonsei University College of Medicine, Seoul, South Korea (H. Lee, Y.H. Suh, K. Lee)Seegene Medical Foundation, Seoul (S. Lee)Samkwang Medical Laboratories, Seoul (Y.-K. Kim); U2 Clinical Laboratories, Seoul (M.-S. Han)Green Cross Laboratories, Yongin, South Korea (H.G. Bae)World Health Organization Collaborating Centre for Gonorrhoea and other STIs, Örebro University, Örebro, Sweden (M. Unemo)


In South Korea, surveillance of antimicrobial drug resistance in Neisseria gonorrhoeae is extremely limited. We describe the emergence and subsequent national spread of N. gonorrhoeae strains with mosaic penA alleles associated with decreased susceptibility and resistance to extended-spectrum cephalosporins. From 2012 through 2017, the proportion of mosaic penA alleles in gonococcal-positive nucleic acid amplification test (NAAT) specimens across South Korea increased from 1.1% to 23.9%. Gonococcal strains with mosaic penA alleles emerged in the international hubs of Seoul in Gyeonggi Province and Busan in South Gyeongsang Province and subsequently spread across South Korea. Most common was mosaic penA-10.001 (n = 572 isolates; 94.7%), which is associated with cefixime resistance. We also identified mosaic penA-34.001 and penA-60.001, both of which are associated with multidrug-resistant gonococcal strains and spread of cefixime and ceftriaxone resistance. Implementation of molecular resistance prediction from N. gonorrhoeae–positive nucleic acid amplification test specimens is imperative in South Korea and internationally.
Neisseria gonorrhoeae remains a major public health threat globally (1). During the past 2 decades, N. gonorrhoeae strains with resistance to extended-spectrum cephalosporins (ESCs), including ceftriaxone, the last remaining option for empiric first-line gonorrhea treatment, have emerged and spread internationally, which is a serious concern worldwide (223). Enhanced global antimicrobial resistance (AMR) surveillance is crucial to controlling further spread of AMR strains. However, in many countries, culture-based AMR testing cannot be performed because most patients are treated empirically at primary-care facilities or hospitals where culture is unavailable and nucleic acid amplification tests (NAATs) have replaced culture for diagnosis. Consequently, molecular methods for detection of AMR determinants in N. gonorrhoeae–positive NAAT specimens are essential (21).
For the currently recommended ESCs, resistance is influenced by many different mutations in various genes that collaboratively increase the MICs of ESCs (4,5,7,24). Nevertheless, the main mechanism for ESC resistance is the acquisition of a mosaic penA allele encoding a mosaic version of the ESC lethal target, penicillin-binding protein 2 (2432). Nearly all N. gonorrhoeae isolates with clinical or in vitro resistance to ESCs contain a mosaic penA allele. However, isolates with decreased susceptibility or susceptibility to ESCs also can contain a mosaic penA allele; that is, different mosaic penA alleles can affect the ESC MICs differently (38,1019,2438). Accordingly, a molecular assay detecting all types of mosaic penA alleles can predict ESC resistance with a high sensitivity but lower specificity (2431). Currently, no molecular assays for detection of mosaic penA alleles and prediction of ESC resistance are commercially available.
In South Korea, ≈15,000 gonorrhea patients (≈29 patients/100,000 population) are reported to the Korean Health Insurance System (http://opendata.hira.or.kr) annually, but culture and AMR testing of N. gonorrhoeae are exceedingly limited (2,3). ESC resistance and ESC-resistant N. gonorrhoeae strains with the mosaic penA-10.001 allele were observed in 2011 in South Korea (3). Since the early 2000s, ESC-resistant N. gonorrhoeaestrains containing the mosaic penA-10.001, associated with cefixime resistance, have been prevalent and caused cefixime treatment failures in Japan (4,5,7,33). In South Korea, molecular prediction of AMR directly from N. gonorrhoeae–positive NAAT samples is imperative for large scale screening and prediction of the level and spread of ESC resistance in Korea.
We describe the emergence and subsequent national spread of N. gonorrhoeae strains with mosaic penA alleles in gonococcal-positive NAAT specimens across South Korea from 2012 through 2017. For molecular epidemiology, penA sequencing, N. gonorrhoeae multiantigen sequence typing (NG-MAST), and multilocus sequence typing (MLST) were performed on all mosaic penA-positive specimens.

Materials and Methods

N. gonorrhoeae–Positive NAAT Specimens
We examined DNA extracts of NAAT specimens, mainly first-voided urine (55%) and vaginal swab specimens (27%), but also urethral and cervical swab specimens (18%), positive for N. gonorrhoeae (n = 3,884; 1 specimen per patient) by Seeplex NAATs (Seegene, http://www.seegene.com). These DNA extracts were obtained from commercial centralized accredited laboratories (2012–2017, Seegene Medical Foundation and SamKwang Medical Laboratories; 2016–2017, U2Bio; and 2017, Green Cross Medical Foundation) in Seoul, South Korea. The DNA extracts were stored at −70°C before further analysis.
All samples were collected and preserved as part of the routine diagnostics, and no patient identification data were available during the study. Therefore, ethics approval was not required.
Detection of Mosaic penA Alleles
We examined all DNA extracts for mosaic penA alleles in 2 steps: 1) screening of all types of mosaic penA alleles (7,34); and 2) detection of the specific mosaic penA alleles that caused high-level resistance to ceftriaxone in the strains H041 (penA-37.001; 7) and F89 (penA-42.001; 6). For screening of all types of mosaic penA alleles, we used a previously described TaqMan probe–based real-time PCR method on a Rotor-Gene 6000 (QIAGEN, https://www.qiagen.com) (34). We then tested all DNA extracts positive for a mosaic penA allele by using a real-time PCR specific for penA-37.001 (35) and a modified hybridization probe-based real-time PCR detecting penA-42.001 (36).
Sequencing of Mosaic penA Alleles
We sequenced the entire penA gene in all DNA specimens positive for a mosaic penA allele, as previously described (3,37). Among 604 mosaic penA-positive specimens, we were able to sequence 601 (99.5%) specimens. Three (0.5%) specimens failed to sequence due to low DNA content. The mosaic penA alleles were named by using the Neisseria gonorrhoeae Sequence Typing for Antimicrobial Resistance (NG-STAR; 38) database (https://ngstar.canada.ca/welcome/home).
Molecular Epidemiologic Characterization
We performed NG-MAST (39) and MLST on all mosaic penA-positive specimens using the methods described on the NG-MAST (http://www.ng-mast.net) and MLST (http://pubmlst.org/neisseria) websites. We successfully typed 594 (98.3%) of the mosaic penA-positive specimens with NG-MAST and 593 (98.2%) specimens with MLST.
Antimicrobial Agents Used for Gonorrhea Treatment
We acquired prescription data for patients treated for gonorrhea from 2010 through 2017 in South Korea from the Korea Health Insurance Review & Assessment Service (http://opendata.hira.or.kr). In South Korea, all clinics and hospitals participate in the national health insurance system, and reporting of prescription data and diagnosis to the Korea Health Insurance Review & Assessment Service HIRA is mandatory. We analyzed the percentage of diagnosed gonococcal infections in patients who did not have additional sexually transmitted infections and who were treated with each specific antimicrobial drug.
Statistical Analysis
We used the Student t‐test and a test of proportions for statistical analysis (Statistica 12.0 PL software, https://www.tibco.com). The level of significance was set at α = 0.05.


N. gonorrhoeae–Positive NAAT Specimens and Corresponding Patients
The collection of DNA extracts we examined consisted of 3,884 N. gonorrhoeae−positive NAAT specimens collected across South Korea from 2012 through 2017 (Table 1). The number of specimens per year varied from 428 in 2012 to 901 in 2017 (Table 1). Data on the sex and age of patients were available for 3,422 (88.1%) of specimens. The ratio of men to women was 1:0.37, and 71.1% of the specimens were collected from patients in their 20s (43.9%) and 30s (27.2%).
Molecular Typing of Gonococcal Mosaic penA-Positive Specimens
The proportion of mosaic penA alleles in the specimens increased annually, from 1.2% (5/428) in 2012 to 23.9% (215/901) in 2017 (Table 2). The mosaic penA-10.001, previously associated with resistance to ESCs, particularly cefixime and other oral ESCs (4,5,33), was the most common mosaic penA allele (n = 572; 94.7% of all mosaic penA-positive specimens). The annual proportion of mosaic penA-10.001 among the mosaic penA-positive specimens varied from 60% to 100% (Table 2). Most (76.9%) mosaic penA-10.001−positive samples belonged to MLST ST1901, but the proportion of sequence type (ST) 1901 among the mosaic penA-10.001 specimens decreased from 100% (5/5) in 2012 to 68% (143/210) in 2017. In contrast, the proportion of other MLST STs, including ST1588 and ST7363, increased. In 2012 and 2013, NG-MAST ST2958 was the most prevalent ST, but the genetically closely related NG-MAST ST10668 was most common in 2014 and 2015. In 2016 and 2017, NG-MAST STs diversified, and ST15014 became the most common.
The mosaic penA-34.001 was found in 1–3 samples annually between 2013 and 2017, and the mosaic penA-72.001 (penA-34.001+P551S) was identified in 10 samples between 2013 and 2016. These mosaic penA alleles also have been previously associated with ESC resistance internationally (4,5,31,40). Of particular concern, mosaic penA-60.001, which has been found in internationally spreading ceftriaxone-resistant gonococcal strains since 2015 (12,1416,18,19), was identified in a sample in 2017. This sample belonged to MLST ST1903 and NG-MAST ST16327 and was collected from a man in Seoul (Table 2).
Distribution of Mosaic penA Alleles in Different Provinces
Thumbnail of Distribution of specimens positive for a Neisseria gonorrhoeae mosaic penA allele, by year and province, South Korea, 2010–2017. Numbers shown in each province denote the proportion of samples positive for a mosaic penA allele in 2012. The bar graphs describe the percentage of specimens positive for a N. gonorrhoeae mosaic penA allele in each province and year. Seoul is the capital city of South Korea, and Gyeonggi Province contains an international airport.
Figure 1. Distribution of specimens positive for a Neisseria gonorrhoeaemosaic penA allele, by year and province, South Korea, 2010–2017. Numbers shown in each province denote the proportion of samples positive for a...
We summarized the annual proportions of mosaic penA alleles in the 10 different provinces of South Korea from 2012 through 2017 (Figure 1). In 2012, only 5 mosaic penA-positive specimens were identified: 1 each in the capital city Seoul (0.5% of the total number of isolates collected in this location), Gyeonggi Province surrounding Seoul (1.4%), and North Gyeongsang Province (2.0%) and 2 (3.1%) in South Gyeongsang Province, which includes Busan, the second largest city in South Korea. In 2013, the proportions of mosaic penA-positive specimens in these 4 provinces increased to 4.1%–7.4%, but no mosaic penA-positive specimens were detected in any of the other provinces. However, in 2014, while the proportion of mosaic penA-positive specimens further increased in these 4 provinces (to 6.4%–16.5%), mosaic penA-positive specimens also emerged in 4 of the 6 additional provinces (proportions of 7.2%–15.4%). Mosaic penA-positive specimens were widely spread in all provinces of South Korea, except Jeju Island, in 2015 (9.7%–34.5%) and 2016 (3.8%–32.0%). In 2017, the proportion of mosaic penA-positive specimens was >20% in all provinces, except South Jeolla Province (13.1%), and mosaic penA-positive specimens also were found on Jeju Island (33.3%) (Figure 1).
Antimicrobial Drugs Used for Gonorrhea Treatment
Thumbnail of Percentages of Neisseria gonorrhoeae mosaic penA in NAAT specimens and gonorrhea patients treated with ceftriaxone or any extended-spectrum cephalosporin, South Korea, 2010–2017. Data for antimicrobial drug use were acquired from the Korea Health Insurance Review and Assessment Service in South Korea. In 2010 and 2011, no NAAT samples were available for screening of mosaic penA alleles. NA, not assessed; NAAT, nucleic acid amplification test.
Figure 2. Percentages of Neisseria gonorrhoeae mosaic penA in NAAT specimens and gonorrhea patients treated with ceftriaxone or any extended-spectrum cephalosporin, South Korea, 2010–2017. Data for antimicrobial drug use were acquired from the...
In 2010, 13.1% of gonorrhea patients were treated with ceftriaxone, and 17.4% were treated with any ESC, but these proportions increased to 30.5% treated with ceftriaxone and 38.8% treated with ESCs in 2017 (Figure 2). With the exception of ceftriaxone, cefixime is the most widely used ESC; the proportion of patients treated with cefixime increased from 2.9% in 2010 to 6.6% in 2017. In 2010, 32.8% of patients were treated with spectinomycin and 49.6% with fluoroquinolone, but these proportions decreased in 2017 to 16.0% treated with spectinomycin and 26.7% treated with fluoroquinolone (Table 3).


The emergence of multidrug-resistant N. gonorrhoeae is compromising the treatment of gonorrhea globally. In most countries, only ESCs, azithromycin combined with ESCs, and spectinomycin remain recommended for the empiric treatment of gonorrhea (35,810,2023,31,32,4144).
Among the ESCs, oral cefixime and the more potent injectable ceftriaxone have been recommended for the treatment of gonorrhea internationally (4,5,10,4144). However, resistance to cefixime emerged in Japan in the early 2000s, and cefixime resistance subsequently has been reported in many countries globally. Since 2010, resistance to ceftriaxone, the last remaining option for empiric first-line monotherapy, also has been described in many countries (319,21,32). The mechanisms of resistance to these ESCs are complex and involve different mutations in the penA gene, in the promoter or coding region of mtrR, and in the porB1b gene. However, the main ESC-resistance determinant is evidently the acquisition of a mosaic penA allele. The mosaic penA-10.001 was shown to be strongly associated with cefixime resistance in gonococcal isolates in Japan in the early 2000s (35,7,33), which resulted in the exclusion of cefixime from the Japanese treatment guidelines in 2006 (43). In South Korea, 1 cefixime-resistant isolate cultured in 2004 (2), 1 in 2011, and 3 in 2013 previously were reported to contain mosaic penA-10.001 (3). In our study, 1.1% (n = 5) of N. gonorrhoeae–positive NAAT specimens contained a mosaic penA allele in 2012. This proportion increased significantly in 2017 to 23.9% (n = 215; p<0.05). The proportion of mosaic penA-positive specimens (n = 209) containing mosaic penA-10.001 was 100% (n = 5) in 2012 and >90% during all the following years, except 2013 (77.8%; 21/27).
Twenty-five (96.2%) of the NAAT specimens containing mosaic penA-10.001 in 2012 and 2013 were collected from the capital region of Seoul and surrounding Gyeonggi Province, as well as the Gyeongsang area (North and South). Seoul (population 8.8 million; 19.8% of the population of South Korea) and the Gyeonggi area (29.6% of the population) share 2 large international airports and an international port, which have pivotal roles in trading with foreign countries, including China and Japan. The South Gyeongsang area (15.6% of the population) includes Ulsan, Gyeonsangnam-do, and the second largest city in South Korea, Busan, which comprises the main port in northeast Asia, with close and frequent trading routes to Japan. In 2012 and 2013, the proportions of mosaic penA-10.001 were 2.2% in the capital area and 4.1% in the Gyeongsang area, whereas the proportion was only 0.8% in the remaining provinces. This indicates that N. gonorrhoeae strains with mosaic penA-10.001, associated with decreased susceptibility and resistance to ESCs, initially emerged or were imported to at least 2 areas (Seoul and Gyeongsang) of South Korea, possibly from Japan, where these strains have been prevalent for many years (4,5,7,33). From 2014, the proportion of N. gonorrhoeae mosaic penA-10.001 in the other provinces significantly increased (from 9.1% in 2014 to 20.9% in 2017; p<0.05); accordingly, N. gonorrhoeae strains with mosaic penA-10.001 spread and diversified, based on NG-MAST STs, nationally in South Korea.
Gonococcal resistance to cefixime and other oral ESCs is common in South Korea, according to a smaller study (3), and the prevalent mosaic penA-10.001 we report causes decreased susceptibility and frequently resistance to cefixime and other oral ESCs (4,5,7,33). With these facts in mind, oral ESCs should be abandoned from the treatment of gonorrhea in South Korea. Gonococcal strains with mosaic penA-10.001 also can cause ceftriaxone treatment failure (17). However, mosaic penA-10.001 affects the MICs of ceftriaxone less than those of cefixime in general. Thus, ceftriaxone, combined with azithromycin, should be the first-line empiric treatment in South Korea. However, N. gonorrhoeae specimens containing mosaic penA-34.001 (n = 11) and penA-72.001 (n = 15) also were detected. These mosaic penA alleles have been associated with ESC resistance globally (4,5,31,40) and require only a single additional penicillin binding protein 2 amino acid alteration (A501P) to develop high-level ceftriaxone resistance (6,45). Of most concern, mosaic penA-60.001 was found in a sample collected in Seoul in 2017 and this mosaic penA allele has been found in internationally spreading ceftriaxone-resistant gonococcal strains in Japan, Australia, Canada, Denmark, France, the United Kingdom, and Ireland since 2015 (12,1416,18,19,46).
The detailed reasons for the emergence and subsequent national spread of gonococcal strains with mosaic penA-10.001 in South Korea are not clear. One possibility is importation of cefixime-resistant gonococcal strains containing the mosaic penA-10.001 from Japan, as previously mentioned. However, increased therapeutic use of ESCs, particularly oral ESCs, in South Korea cannot be excluded as contributing to the increased spread of ESC-resistant gonococcal strains. In South Korea, spectinomycin, fluoroquinolones, and ESCs have been used to treat gonorrhea since the early 2000s (3). In 2002, most patients were treated with spectinomycin; only 8.4% of all patients were treated with ceftriaxone, and oral ESCs were rarely prescribed. However, 38.8% of patients were treated with ESCs (30.5% with ceftriaxone) in 2017. Despite the high use of spectinomycin in South Korea for decades, susceptibility to spectinomycin in N. gonorrhoeae has remained high. No spectinomycin-resistant gonococcal strain has been reported since 1993. Nevertheless, spectinomycin has a low eradication rate for pharyngeal gonorrhea (47) and, if pharyngeal infection has not been excluded, should be used only in dual antimicrobial therapy (e.g., in combination with azithromycin). In the 2016 South Korea guideline, dual antimicrobial therapy (ceftriaxone 500 mg or 1 g plus azithromycin 1 g) is the recommended first-line empiric therapy for uncomplicated gonorrhea (48).
The limitations of our study include the unavailability of gonococcal isolates, MIC data, rectal or pharyngeal specimens (which are not covered by the national insurance system in South Korea), and clinical or epidemiologic information regarding patients or treatment outcome. Accordingly, phenotypic ESC resistance was not measured; instead molecular detection of mosaic penA alleles was used to reflect the ESC resistance. Nearly all gonococcal strains with ESC resistance contain a mosaic penA allele; however, isolates with decreased susceptibility or susceptibility to ESCs also can contain a mosaic penA allele, and different mosaic penA alleles affect the ESC MICs differently (38,1019,2438). Even so, the mosaic penA-10.001 in 94.7% of our mosaic penA allele-positive specimens is relatively strongly associated with resistance to cefixime particularly and other oral ESCs, although with a weaker association with ceftriaxone resistance. In addition, our molecular approach to identifying decreased susceptibility and resistance to ESCs can overestimate the ESC resistance. The mosaic penA allele PCR assay (34) did not include any internal control for gonococcal DNA. However, the assay has been previously evaluated on gonococcal strains and detects 1–10 copies of a mosaic penA per PCR reaction (34) on urogenital and extragenital gonococcal-positive and gonococcal-negative NAAT samples (49,50). Finally, the distribution of specimens could not be allocated by population in an ideal way and the suboptimal number of samples, especially from the Gyeonggi area, Jeju, Gangwon, South Gyeongsang, South Jeolla, and North Jeolla provinces in some years, might have underestimated the prevalence of N. gonorrhoeae specimens with mosaic penA allele in these provinces.
In conclusion, we describe the initial emergence and subsequent national spread of N. gonorrhoeae strains with mosaic penA alleles (penA-10.001 in 94.7% of specimens), associated with decreased susceptibility and resistance to ESCs, in NAAT specimens collected across South Korea from 2012 through 2017. The proportion of penA-10.001, frequently causing resistance to cefixime and other oral ESCs, increased during these years. Only a few additional mutations in penA-10.001 are required for development of high-level resistance to ceftriaxone. Furthermore, we identified mosaic penA-34.001 and penA-60.001, both associated with multidrug-resistant gonorrhea and international spread of cefixime and ceftriaxone resistance (4,5,12,1416,18,19,31,40). The mosaic penA-60.001 has been found in internationally spreading ceftriaxone-resistant gonococcal strains in Japan, Australia, Canada, Denmark, the United Kingdom, France, and Ireland since 2015 (12,1416,18,19,46). Nevertheless, N. gonorrhoeae strains with ESC resistance due to nonmosaic penA alleles, such as penA-13.001, also are spreading in South Korea (3). Consequently, it is essential to establish a systematic, regular, and quality-assured phenotypic AMR surveillance system for N. gonorrhoeae in South Korea. Implementing the use of molecular methods for prediction of AMR or antimicrobial susceptibility is also crucial. These molecular methods will effectively support phenotypic AMR surveillance and enable large-scale screening of N. gonorrhoeae–positive NAAT specimens, which represent most of N. gonorrhoeae diagnostic specimens in many countries internationally.
Dr. Hyukmin Lee is an associate professor and senior researcher at the Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea. His primary research interest is clinical microbiology and molecular biology, with a special focus on gonorrhea.
This study was supported by grants from the National Institutes of Health (2016-ER4401), Korea Center for Disease Control and Prevention (2017-ER4401-01), Ministry of Health and Welfare, Republic of Korea.


This work was performed at the Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea. This study was supported by grants from the National Institute of Health, Korea Centers for Disease Control and Prevention, Ministry of Health and Welfare, Republic of Korea.
H.L., M.U., and K.L. designed and initiated the study; S.L., Y.-K.K., M.-S.H. and H.G.B. provided NAAT specimens; H.L., S.L., Y.-K.K., and Y.H.S. coordinated and performed all the laboratory analyses; H.L., K.L., and M.U. analyzed and interpreted all the data and wrote a first draft of the paper; and all authors read, commented on, and approved the final manuscript.


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Cite This Article

DOI: 10.3201/eid2503.181503
1Joint senior authors.

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