Volume 22, Number 11—November 2016
Dispatch
Guinea Worm (Dracunculus medinensis) Infection in a Wild-Caught Frog, Chad
On This Page
Mark L. Eberhard
, Christopher A. Cleveland, Hubert Zirimwabagabo, Michael J. Yabsley, Philippe Tchindebet Ouakou, and Ernesto Ruiz-Tiben
, Christopher A. Cleveland, Hubert Zirimwabagabo, Michael J. Yabsley, Philippe Tchindebet Ouakou, and Ernesto Ruiz-TibenAbstract
A third-stage (infective) larva of Dracunculus medinensis, the causative agent of Guinea worm disease, was recovered from a wild-caught Phrynobatrachus francisci frog in Chad. Although green frogs (Lithobates clamitans) have been experimentally infected with D. medinensis worms, our findings prove that frogs can serve as natural paratenic hosts.
The peculiar epidemiology of Dracunculus medinensis (Guinea worm), the causative agent of dracunculiasis (Guinea worm disease), in Chad has led to speculation that a paratenic host is involved in the life cycle, most likely an animal with an aquatic stage that would feed upon copepods and harbor the infection for subsequent transmission to a human or dog definitive host (1). Recent experiments demonstrated that D. medinensis worms, like the closely related parasite D. insignis, could utilize green frog (Lithobates clamitans) tadpoles as a paratenic host (2). During June and July 2016, a survey of potential D. medinensisworm paratenic hosts was conducted in Chad. The study area was located in southern Chad near the small village of Marabe (Moyen Chari region, Kyabe district), along the upper reaches of the Chari River, where many cases of dracunculiasis in dogs have been recorded; the closest large town to Marabe is Sarh (3).
We used standard procedures, as previously described (1), to examine muscle and viscera of 88 frogs from the study area; the frogs, which were of several sizes and species (i.e., Ranidae, Pipidae, Phrynobatrachidae, Bufonidae), were collected by local villagers and fishermen. In brief, the viscera was removed and placed in water for at least 1 h before being examined by microscope for motile nematode larvae. The musculature and carcass were bluntly dissected and similarly placed in water for at least 1 h before the solution was examined for motile nematode larvae.
Figure. Size and shape of aDracunculus medinensis third-stage larva recovered from aPhrynobatrachus francisci frog from Chad. Scale bar indicates 25 μm. Inset shows detailed morphology of the tip of the tail of...
We observed 1–5 nematode larvae in 6 (7%) of the 88 frogs. Morphologically similar larvae were collected from the viscera washing of 5 of the 6 frogs; these larvae were identified as pinworms, based on morphologic characteristics and comparison to larvae released by a female oxyurid collected from the gut. However, upon subsequent microscope examination, 1 larva from the muscle and carcass washings of a single mature frog was found to be morphologically consistent with Dracunculus species, including size, distinct cuticular striation, and, most notably, a 3-lobed tail (Figure). We preserved the larva in ethanol and then extracted DNA and amplified a partial cytochrome c oxidase subunit I gene by PCR (4). Partial sequencing (187 bp) showed that the larva shared 99.5% similarity with D. medinensis isolates in GenBank (accession nos. LK978189 and KF770021– KF770024), confirming its identity as D. medinensis. The sequence shared only 95.2% similarity with D. insignis and 91.9% similarity with D. lutrae (GenBank accession nos. EU646534 and EU646602, respectively).
To confirm the species identity of the frog, we extracted DNA from ethanol-fixed tissue and amplified the 16S ribosomal RNA gene (2). The sequence (450 bp) indicated that the frog was a ranid species in the genusPhrynobatrachus, most likely P. francisci because it shared 99% similarity with P. francisci isolates in GenBank (accession nos. GU457546–GU457549, EU71820, and AY902377).
Tadpoles and frogs have long been known to experimentally support infective larvae of D. insignis (5–7), and just recently, they have been shown to experimentally support infective larvae of D. medinensis (2); however, natural infection with Dracunculus species has not previously been documented in any wild-caught amphibian. The finding of a wild-caught frog harboring a natural infection with a D. medinensis larva validates the findings of these experimental infections and demonstrates that such a paratenic host is likely involved in the transmission of D. medinensislarvae in Chad. This finding is especially noteworthy at this point because the Guinea worm eradication program has reduced the number of countries with endemic Guinea worm disease from 20 to 4 and the number of persons infected each year from >1 million to <20. To be uncovering this aspect of the D. medinensis life cycle, the description of which was published >145 years ago (8) and remained relatively unchanged to date, further highlights the need to continue field research, even at the end of an eradication campaign.
Given the diversity of frog species (i.e., families Ranidae, Pipidae, and Phrynobatrachidae) that can be experimentally infected with D. medinensis or D. insignis worms, it seems probable that natural Dracunculus infections are not limited to frogs of the genus Phrynobatrachus but may well include numerous other ranids and highly aquatic Xenopus species frogs (African clawed frogs), which are common and native to Chad. Additional surveillance is needed to detail the prevalence and burden of infection among frogs in Chad as well as the diversity of natural hosts. These data do not address whether all transmission occurring in humans and dogs in Chad are a result of consumption of a paratenic host, such as a frog, but the peculiar epidemiology of D. medinensis worms in Chad clearly suggests that traditional drinking water sources are not the primary source of infection.
Our findings confirm that an appropriate wild-caught paratenic host in Chad was infected with a D. medinensis larva, and they corroborate findings of experimental studies that suggested the possible inclusion of an amphibian paratenic host in the maintenance of D. medinensis worms in nature. We conclude that paratenic hosts, specifically frogs, may facilitate transmission of D. medinensis worms to humans and dogs in Chad via consumption of poorly cooked or raw food items.
Dr. Eberhard, a retired parasitologist, is a guest researcher in the Parasitic Diseases Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. He has a broad interest in parasite life cycles and transmission dynamics and has been engaged in the Guinea worm eradication program since 1986.
Acknowledgments
We thank Melinda Denson for facilitating transport and local arrangements in support of our field work and Jordan Schermerhorn and Robert Hartwig for their assistance.
During 2008–2015, The Carter Center’s work to eradicate Guinea worm disease was made possible by financial and in-kind contributions from many donors. A full listing of supporters can be found at The Carter Center website (http://www.cartercenter.org/donate/corporate-government-foundation-partners/index.html).
References
- Eberhard ML, Ruiz-Tiben E, Hopkins DR, Farrell C, Toe F, Weiss A, The peculiar epidemiology of dracunculiasis in Chad. Am J Trop Med Hyg.2014;90:61–70.DOIPubMed
- Eberhard ML, Yabsley MJ, Zirimwabagabo H, Bishop H, Cleveland CA, Maerz JC, Possible role of fish and frogs as paratenic hosts of Dracunculus medinensis, Chad. Emerg Infect Dis. 2016;22:1428–30.DOIPubMed
- US Department of Health and Human Services. Guinea worm wrap-up #239. March 25, 2016 [cited 2016 Aug 5].https://www.cartercenter.org/resources/pdfs/news/health_publications/guinea_worm/wrap-up/239.pdf
- Elsasser SC, Floyd R, Hebert PD, Schulte-Hostedde AI. Species identification of North American guinea worms (Nematoda: Dracunculus) with DNA barcoding. Mol Ecol Resour. 2009;9:707–12.DOIPubMed
- Crichton VFJ, Beverley-Burton M. Observations on the seasonal prevalence, pathology and transmission of Dracunculus insignis (Nematoda: Dracunculoidea) in the raccoon (Procyon lotor (L.) in Ontario. J Wildl Dis. 1977;13:273–80.DOIPubMed
- Eberhard ML, Brandt FH. The role of tadpoles and frogs as paratenic hosts in the life cycle of Dracunculus insignis (Nematoda: Dracunculoidea). J Parasitol. 1995;81:792–3.DOIPubMed
- Anderson RC. Nematode parasites of vertebrates: their development and transmission. Wallingford (UK): CAB International; 1992. p. 320–35.
- Naust E. Concerning the structure and reproduction of the guinea worm (Filaria medinensis L.) by Aleksej Pavlovich Fedchenko, 1890. Am J Trop Med Hyg. 1971;20:511–23.PubMed
Figure
Suggested citation for this article: Eberhard ML, Cleveland CA, Zirimwabagabo H, Yabsley MJ, Ouakou PT, Ruiz-Tiben E. Guinea Worm (Dracunculus medinensis) infection in a wild-caught frog, Chad. Emerg Infect Dis. 2016 Sep [date cited]. http://dx.doi.org/10.3201/eid2211.161332
.png)
No hay comentarios:
Publicar un comentario