Ebola Virus in Breast Milk in an Ebola Virus–Positive Mother with Twin Babies, Guinea, 2015

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To the Editor: Field clinicians working during the unprecedented Ebola virus disease (EVD) outbreak in West Africa, which began in December 2013, have been confronted with complex situations concerning mothers and breast-fed children in which one or both in a pair have tested positive for Ebola virus (EBOV) (1). More data, especially regarding virus shedding in breast milk, is critical to provide better care and guidance in future outbreaks. We report the case of a lactating EBOV-positive mother and her twin babies. The case is anonymously reported with the mother’s consent. The study met the Médecins Sans Frontières Ethics Review Board and approved criteria for studies of routinely collected data.

In Guinea in 2015, a woman and her 4-month-old twins (baby 1 and 2) were registered as contacts after the woman’s mother tested positive for EBOV (postmortem diagnosis by reverse transcription PCR [RT-PCR]). The woman and her babies, who were exclusively breast-fed, were followed daily by contact tracers. When baby 1 became febrile, the woman left her home to seek help from a traditional healer, bringing both twins with her. A few days later, baby 1 died and was buried without EBOV testing; according to the World Health Organization case definition, baby 1 was a probable EVD case-patient (2).

Eleven days after baby 1 died, the woman became sick; 5 days later, she was admitted to an Ebola treatment center. At admission (day 0), she had headache, loss of appetite, abdominal pain, joint pain, dysphagia, conjunctival injection, and myalgia but was afebrile. On day 1, a blood sample from the woman was positive for EBOV by RT-PCR (Xpert Ebola Assay, GeneXpert Instrument Systems; Cepheid, Sunnyvale, CA, USA) with a cycle threshold (Ct) of 32.5. Baby 2 tested negative for EBOV on day 1 and 72 hours later. Baby 2 was tested twice because he was considered at high risk for infection after being breast-fed for 6 days while his mother was symptomatic (i.e., until day 1 of her hospital admission).

On day 1, the woman was given convalescent-phase plasma from EBOV survivors; the treatment was given according to a compassionate-use protocol and was the standard process in this center at the time. On day 6, breast milk was sampled and tested positive for EBOV (Ct 21.6) (Table). The woman’s clinical course was favorable; she remained afebrile during hospitalization, but mild symptoms persisted until day 5. The first convalescent-phase test, done on day 14, showed Ct values of 40.5 and 27.5 for blood and breast milk, respectively. On day 21, a second breast milk sample tested positive (Ct 32.7). On day 24, the woman was given cabergoline (0.5 mg 2×/d for 2 days) to cease lactation, after which no more breast milk samples could be collected. On day 29 after admission, she tested negative for EBOV in blood and urine and was reunited with baby 2. Serologic testing for baby 2 was done on day 23 and showed no sign of previous subclinical infection (ELISA, IgM, and IgG negative).

Many questions in this case remain unanswered, but our findings show the potential infectivity of breast milk for at least 26 days after EVD symptom onset and demonstrate a case in which a baby was not infected by breast milk from his EBOV-positive mother. However, it should be noted that the woman’s breast milk was never tested while she was breast-feeding baby 2.

The literature on EBOV in breast milk of EBOV-positive patients is extremely scarce (3). In a previous study from the 2000 Sudan EBOV outbreak in Gulu, Uganda, breast milk from a convalescent-phase patient was sampled 15 days after symptom onset and tested positive for EBOV by RT-PCR and virus culture (4). Another study conducted in Guinea during the current outbreak, reported a mother–baby pair in which EVD developed in the baby 14 days after symptom onset in the mother, but breast milk from the mother sampled 17 days after symptom onset was negative by EBOV RT-PCR (1).

It is unclear whether infectious virus or defective particles are being secreted in breast milk. Ct values were consistently lower in breast milk than in blood when tested concomitantly, but in this case, breast milk samples were not collected until day 6. Our findings suggest that breast milk is infected by EBOV at a later stage of the disease than blood but then follows the expected replication kinetics observed in venous blood.

Considering the high EVD death rate, until further evidence is found, we recommend that EBOV-positive women stop breast-feeding immediately and that breast-feeding not be resumed until 2 negative RT-PCR tests of the breast milk have been confirmed. This suggestion is in line with the World Health Organization recommendation for testing semen in male EVD survivors (5). The public health risk for EBOV to remain in breast milk for at least 26 days after EVD symptom onset and for breast milk to possibly be infectious after a patient has cleared the virus from the blood should also be acknowledged.

Helena Nordenstedt

, Elhadj Ibrahima Bah, Marc-Antoine de la Vega, Mamadou Barry, Magassouba N’Faly, Moumié Barry, Beatrice Crahay, Tom Decroo, Michel Van Herp, and Brecht Ingelbeen

Author affiliations: Karolinska Institutet, Stockholm, Sweden (H. Nordenstedt); Médecins Sans Frontières Operational Centre Brussels, Conakry, Guinea (H. Nordenstedt, E.I. Bah, M.-A. de la Vega, Mamadou Barry, B. Crahay, B. Ingelbeen); Donka National Hospital, Conakry (E.I. Bah, Moumié Barry);University of Manitoba, Winnipeg, Manitoba, Canada (M.-A. de la Vega); Université Gamal Abdel Nasser, Conakry (M. N’Faly); Médecins Sans Frontières Operational Centre Brussels, Brussels, Belgium (T. Decroo, M. Van Herp); Institute of Tropical Medicine, Antwerp, Belgium (B. Ingelbeen)

References

Moreau M, Spencer C, Gozalbes JG, Colebunders R, Lefevre A, Gryseels S, Lactating mothers infected with Ebola virus: EBOV RT-PCR of blood only may be insufficient. Euro Surveill. 2015;20: pii=21017.PubMed
World Health Organization. Interim guideline: case definition recommendations for Ebola or Marburg virus diseases. 2014 Aug 9 [cited 2015 Nov 16]. http://apps.who.int/iris/bitstream/10665/146397/1/WHO_EVD_CaseDef_14.1_eng.pdf?ua=1
Osterholm MT, Moore KA, Kelley NS, Brosseau LM, Wong G, Murphy FA, Transmission of Ebola viruses: what we know and what we do not know.MBio. 2015;6:e00137.PubMed
Bausch DG, Towner JS, Dowell SF, Kaducu F, Lukwiya M, Sanchez A, Assessment of the risk of Ebola virus transmission from bodily fluids and fomites. J Infect Dis. 2007;196(Suppl 2):S142–7. DOI PubMed
World Health Organization. Sexual and reproductive health. Interim advice on the sexual transmission of the Ebola virus disease. 2015 May 8 [cited 2016 Jan 1]. http://www.who.int/reproductivehealth/topics/rtis/ebola-virus-semen/en/

Table

Table. Overview of results from all Ebola virus RT-PCRs performed during hospitalization of breast-feeding mother of twin babies, Guinea, 2015

Suggested citation for this article: Nordenstedt H, Bah EI, de la Vega MA, Barry M, N’Faly M, Barry M, et al. Ebola Virus in breast milk in an Ebola virus–positive mother, with twin babies, Guinea, 2015 [letter]. Emerg Infect Dis. 2016 Mar [date cited]. http://dx.doi.org/10.3201/eid2204.151880

DOI: 10.3201/eid2204.151880