martes, 9 de junio de 2015

Ahead of Print -Distinct Lineages of Bufavirus in Wild Shrews and Nonhuman Primates - Volume 21, Number 7—July 2015 - Emerging Infectious Disease journal - CDC


Ahead of Print -Distinct Lineages of Bufavirus in Wild Shrews and Nonhuman Primates - Volume 21, Number 7—July 2015 - Emerging Infectious Disease journal - CDC

CDC. Centers for Disease Control and Prevention. CDC 24/7: Saving Lives. Protecting People.

Volume 21, Number 7—July 2015


Distinct Lineages of Bufavirus in Wild Shrews and Nonhuman Primates



Technical Appendicies


Michihito Sasaki, Yasuko Orba, Paulina D. Anindita, Akihiro Ishii, Keisuke Ueno1, Bernard M. Hang’ombe, Aaron S. Mweene, Kimihito Ito, and Hirofumi SawaComments to Author 
Author affiliations: Hokkaido University, Sapporo, Japan (M. Sasaki, Y. Orba, P.D. Anindita, A. Ishii, K. Ueno, K. Ito, H. Sawa)University of Zambia, Lusaka, Zambia (B.M. Hang’ombe, A.S. Mweene)


Viral metagenomic analysis identified a new parvovirus genome in the intestinal contents of wild shrews in Zambia. Related viruses were detected in spleen tissues from wild shrews and nonhuman primates. Phylogenetic analyses showed that these viruses are related to human bufaviruses, highlighting the presence and genetic diversity of bufaviruses in wildlife.
Bufavirus (BuV), a recently described parvovirus, was initially discovered in the feces of a child with diarrhea in Burkina Faso in 2012 (1). Thereafter, BuV was identified in fecal samples from children and adults with gastroenteritis in Bhutan, Finland, and the Netherlands in 2014 (24), respectively. Genome sequences and phylogenetic analyses revealed that BuV comprised at least 3 genotypes and was distinct from all other known members of the Parvoviridae family (1,2). The International Committee on Taxonomy of Viruses assigned BuV as a new species of the genus Protoparvovirus in the subfamily Parvovirinae (5). Whether BuV is an etiologic agent of human gastroenteritis remains unclear, but knowledge about its distribution and genetic divergence in humans is accumulating. However, whether BuV infection exists in wildlife remains unanswered. Through use of metagenomics, we previously described the enteric virome of wild shrews of the Crocidura genus sampled at Mpulungu, Zambia, in 2012 (6). From this sequence dataset (GenBank/EMBL/DDBJ accession no. DRA002561), we identified sequence reads related to BuV. Here, we describe the genome of this new parvovirus.

Dr. Sasaki is a veterinary researcher at the Research Center for Zoonosis Control, Hokkaido University, and is certified as a zoonosis control expert by Hokkaido University. His research interests include the molecular basis of viral pathogenesis.


We thank the Zambia Wildlife Authority for its support with this research.
This work was supported by the Global Institution for Collaborative Research and Education (GI-CoRE) and the Japan Initiative for Global Research Network of Infectious Diseases (J-GRID) from the Japan Ministry of Education, Culture, Sports, Science, and Technology and Grants-in-Aid for Scientific Research (KAKENHI, grant no. 24405043) from the Japan Society for the Promotion of Science.


  1. Phan TGVo NPBonkoungou IJKapoor ABarro NO’Ryan MAcute diarrhea in West African children: diverse enteric viruses and a novel parvovirus genus. J Virol2012;86:1102430 . DOIPubMed
  2. Yahiro TWangchuk STshering KBandhari PZangmo SDorji TNovel human bufavirus genotype 3 in children with severe diarrhea, Bhutan. Emerg Infect Dis2014;20:10379DOIPubMed
  3. Väisänen EKuisma IPhan TGDelwart ELappalainen MTarkka EBufavirus in feces of patients with gastroenteritis, Finland. Emerg Infect Dis.2014;20:107780.PubMed
  4. Smits SLSchapendonk CMvan Beek JVennema HSchürch ACSchipper DNew viruses in idiopathic human diarrhea cases, the Netherlands.Emerg Infect Dis2014;20:121822DOIPubMed
  5. Cotmore SFAgbandje-McKenna MChiorini JAMukha DVPintel DJQiu JThe family Parvoviridae. Arch Virol2014;159:123947.DOIPubMed
  6. Sasaki MOrba YUeno KIshii AMoonga LHang'ombe BMMetagenomic analysis of the shrew enteric virome reveals novel viruses related to human stool-associated viruses. J Gen Virol2015;96:44052DOIPubMed
  7. Handley SAThackray LBZhao GPresti RMiller ADDroit LPathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome. Cell2012;151:25366 . DOIPubMed
  8. Walker SLWonderling RSOwens RAMutational analysis of the adeno-associated virus type 2 Rep68 protein helicase motifs. J Virol.1997;71:69967004 .PubMed
  9. Farr GAZhang LGTattersall PParvoviral virions deploy a capsid-tethered lipolytic enzyme to breach the endosomal membrane during cell entry.Proc Natl Acad Sci U S A2005;102:1714853DOIPubMed
  10. Kivovich VGilbert LVuento MNaides SJThe putative metal coordination motif in the endonuclease domain of human parvovirus B19 NS1 is critical for NS1 induced S phase arrest and DNA damage. Int J Biol Sci2012;8:7992DOIPubMed
  11. Castellanos MPérez RRodríguez-Huete AGrueso EAlmendral JMMateu MGA slender tract of glycine residues is required for translocation of the VP2 protein N-terminal domain through the parvovirus MVM capsid channel to initiate infection. Biochem J2013;455:8794DOIPubMed
  12. Deng XDong YYi QHuang YZhao DYang YThe determinants for the enzyme activity of human parvovirus B19 phospholipase A2 (PLA2) and its influence on cultured cells. PLoS ONE2013;8:e61440DOIPubMed
  13. Sasaki MIshii AOrba YThomas YHang’ombe BMMoonga LHuman parainfluenza virus type 3 in wild nonhuman primates, Zambia. Emerg Infect Dis2013;19:••• . DOIPubMed
  14. Orba YSasaki MYamaguchi HIshii AThomas YHang’ombe BMOrthopoxvirus infection among wildlife in Zambia. J Gen Virol2015;96:3904.DOIPubMed



Technical Appendix

Suggested citation for this article: Sasaki M, Orba Y, Anindita PD, Ishii A, Ueno K, Hang’ombe BM, et al. Distinct lineages of bufavirus in wild shrews and nonhuman primates. Emerg Infect Dis. 2015 Jul [date cited].
DOI: 10.3201/eid2107.141969
1Current affiliation: King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.

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