Detection of Metallo-beta-lactamase Producing Pseudomonas aeruginosa in an Abidjan Hospital, Côte d’Ivoire

Detection of Metallo-beta-lactamase Producing Pseudomonas aeruginosa in an Abidjan Hospital, Côte d’Ivoire

Original-research-article

Detection of Metallo-beta-lactamase Producing Pseudomonas aeruginosa in an Abidjan Hospital, Côte d’Ivoire

Karine M. K. Gba1,2*, Nathalie K. Guessennd2,  Nicole P. Nieko Dangui Makaya1,2,3, Eric J. Tahou1,2, Fernique Konan2, Anatole A. Toty2 and Simon-Pierre A. N’guetta1

1Laboratoire de Génétique, UFR Biosciences, Université Felix Houphouët Boigny, 22BP 582, Abidjan 22, Côte d’Ivoire.

2Institut Pasteur de Côte d'Ivoire, CHU Cocody-01BP 490 Abidjan 01, Côte d’Ivoire.

3Département de Sciences de la Vie, Université Marien Ngouabi, Ecole Normale Supérieure, Brazzaville, République du Congo.

Article Information

Editor(s):

(1) Simone Aquino, Professor, Universidade Nove de Julho, São Paulo, Brazil.

Reviewers:

(1) Ephraim Ehidiamen Ibadin, University of Benin Teaching Hospital, Nigeria.

(2) B. M. Andersen, Oslo University Hospital, Norway.

Complete Peer review History: http://www.sciencedomain.org/review-history/22785

Abstracts

Metallo-beta-lactamase (MBL) producing Pseudomonas aeruginosa has emerged as a threat to hospital infection control, due to its ability of multi-drug resistance. This study was carried out to determine the prevalence of MBL producing P. aeruginosa in an Abidjan Hospital, Côte d'Ivoire.

Methods: A prospective study was undertaken to detect MBLs in P. aeruginosa isolates obtained from various clinical samples. A total of 88 strains were screened for imipenem resistance by the disc diffusion method. Detection of MBLs were further done by phenotypic methods, using imipenem-EDTA disk synergy (DST) test and combined disk test (CDT).

Results: Out of 88 isolates, 14 isolates (15.9%) were imipenem resistant. Out 14 imipenem resistant P. aeruginosa strains, 11 were positive for MBL production by CDT and by DST.

Conclusion: Majority (78.6%) of imipenem resistant P. aeruginosa were MBL positive. The prevalence of MBL producing-P. aeruginosa increased from 10.4% in 2011 to 12.5% in 2015 in Abidjan, Côte d'Ivoire. Early detection of carbapenemases and MBL can be performed in hospitals and laboratories from the following rapid and less expensive tests.

Keywords :

Metallo-beta-lactamases; Pseudomonas aeruginosa; multidrug resistance.

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DOI : 10.9734/JAMB/2018/38792