jueves, 19 de marzo de 2015

Safety of Recombinant VSV–Ebola Virus Vaccine Vector in Pigs - Volume 21, Number 4—April 2015 - Emerging Infectious Disease journal - CDC


Safety of Recombinant VSV–Ebola Virus Vaccine Vector in Pigs - Volume 21, Number 4—April 2015 - Emerging Infectious Disease journal - CDC

Volume 21, Number 4—April 2015


Safety of Recombinant VSV–Ebola Virus Vaccine Vector in Pigs

Emmie de Wit, Andrea Marzi, Trenton Bushmaker, Doug Brining1, Dana Scott, Juergen A. Richt, Thomas W. Geisbert, and Heinz FeldmannComments to Author 
Author affiliations: National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA (E. de Wit, A. Marzi, T. Bushmaker, D. Brining, D. Scott, H. Feldmann)Kansas State University College of Veterinary Medicine, Manhattan, Kansas, USA (J.A. Richt)University of Texas Medical Branch, Galveston, Texas, USA (T.W. Geisbert);University of Manitoba, Winnipeg, Manitoba, Canada (H. Feldmann)


The ongoing Ebola outbreak in West Africa has resulted in fast-track development of vaccine candidates. We tested a vesicular stomatitis virus vector expressing Ebola virus glycoprotein for safety in pigs. Inoculation did not cause disease and vaccine virus shedding was minimal, which indicated that the vaccine virus does not pose a risk of dissemination in pigs.
The current Ebola virus (EBOV) outbreak in West Africa has shown the need for an effective vaccine against this virus. As a result, clinical trials to test several vaccine candidates have been expedited (1) in hopes of contributing to containment of the outbreak. One of these vaccine candidates is based on a recombinant vesiculovirus vector, species vesicular stomatitis Indiana virus (here designated and more commonly known as VSV) expressing the EBOV strain Mayinga glycoprotein (here designated rVSV∆G/EBOVGP; formerly designated VSV∆G/ZEBOVGP) (24). This vaccine was highly efficacious in preexposure and postexposure studies in nonhuman primates after a single injection (5). In addition, the vaccine has been shown to be safe in simian HIV–infected rhesus macaques (6) and was not neurovirulent after intrathalamic inoculation into macaques (7).
However, because VSV is a World Organisation for Animal Health–listed pathogen (8), concerns might arise with regard to spillover of the vaccine vector to livestock when this vaccine is used on a larger scale in humans. To evaluate the safety of rVSV∆G/EBOVGP in a relevant livestock species, we inoculated pigs with this vaccine and compared clinical signs and virus replication with those of a recombinant wild-type VSV vector (rVSVwt) described previously (3).

Dr. de Wit is a research fellow in the Disease Modeling and Transmission Section of the Laboratory of Virology, Rocky Mountain Laboratories, Hamilton, Montana. Her research interests are pathogenesis and transmission of zoonotic emerging viruses.


We thank David Stallknecht, Lisa Kercher, and the Rocky Mountain Veterinary Branch for providing helpful advice.
This study was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health.


  1. Arie STrial of Ebola virus vaccine is due to start next week. BMJ2014;349:g5562 . DOIPubMed
  2. Garbutt MLiebscher RWahl-Jensen VJones SMoller PWagner RProperties of replication-competent vesicular stomatitis virus vectors expressing glycoproteins of filoviruses and arenaviruses. J Virol2004;78:545865DOIPubMed
  3. Lawson NDStillman EAWhitt MARose JKRecombinant vesicular stomatitis viruses from DNA. Proc Natl Acad Sci U S A1995;92:447781.DOIPubMed
  4. Jones SMFeldmann HStroher UGeisbert JBFernando LGrolla ALive attenuated recombinant vaccine protects nonhuman primates against Ebola and Marburg viruses. Nat Med2005;11:78690DOIPubMed
  5. Geisbert TWFeldmann HRecombinant vesicular stomatitis virus–based vaccines against Ebola and Marburg virus infections. J Infect Dis.2011;204(Suppl 3):S107581DOIPubMed
  6. Geisbert TWDaddario-Dicaprio KMLewis MGGeisbert JBGrolla ALeung AVesicular stomatitis virus–based Ebola vaccine is well-tolerated and protects immunocompromised nonhuman primates. PLoS Pathog2008;4:e1000225DOIPubMed
  7. Mire CEMiller ADCarville AWestmoreland SVGeisbert JBMansfield KGRecombinant vesicular stomatitis virus vaccine vectors expressing filovirus glycoproteins lack neurovirulence in nonhuman promates. PLoS Negl Trop Dis. 2012;6:e1567. Epub 2012 Mar 20.
  8. World Organisation for Animal Health (OIE). OIE-listed diseases, infections and infestations in force in 2014 [cited 2015 Jan 20].http://www.oie.int/en/animal-health-in-the-world/oie-listed-diseases-2014/
  9. Stallknecht DEGreer JBMurphy MDMead DGHowerth EWEffect of strain and serotype of vesicular stomatitis virus on viral shedding, vesicular lesion development, and contact transmission in pigs. Am J Vet Res2004;65:12339DOIPubMed
  10. Marzi AEbihara HCallison JGroseth AWilliams KJGeisbert TWVesicular stomatitis virus–based Ebola vaccines with improved cross-protective efficacy. J Infect Dis2011;204(Suppl 3):S106674DOIPubMed
  11. Nakayama EYokoyama AMiyamoto HIgarashi MKishida NMatsuno KEnzyme-linked immunosorbent assay for the detection of filovirus species-specific antibodies. Clin Vaccine Immunol2010;17:17238DOIPubMed


Suggested citation for this article: de Wit E, Marzi A, Bushmaker T, Brining D, Scott D, Richt JA, et al. Safety of recombinant VSV–Ebola virus vaccine vector in pigs. Emerg Infect Dis. 2015 Apr [date cited]. http://dx.doi.org/10.3201/eid2104.142012
DOI: 10.3201/eid2104.142012
1Current affiliation: University of Colorado, Boulder, Colorado, USA.

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