Candidate New Rotavirus Species in Sheltered Dogs, Hungary - Volume 21, Number 4—April 2015 - Emerging Infectious Disease journal - CDC
Volume 21, Number 4—April 2015
Candidate New Rotavirus Species in Sheltered Dogs, Hungary
Rotaviruses (family Reoviridae, genus Rotavirus) are major causes of acute dehydrating gastroenteritis in birds and mammals (1). Rotaviruses have an 11-segmented dsRNA genome encoding 6 structural proteins (viral protein [VP] 1–4, VP6, and VP7) and at least 5 functional nonstructural proteins (NSPs; NSP1–NSP5) (Technical Appendix[PDF - 738 KB - 11 pages] Table 1). Traditionally, rotaviruses have been classified into (sero)groups on the basis of major antigenic differences that predominantly reside in the VP6 and of the genomic RNA profile obtained by polyacrylamide gel electrophoresis and silver staining (1). Recently, a VP6 gene sequence–based classification scheme has been proposed to replace the conventional methods. An empirical 53% aa identity was demonstrated to reliably distinguish strains of various rotaviruses groups (2). Also, reclassification of the 8 rotavirus groups as distinct species within the Rotavirus genus, designated Rotavirus A–H, has been proposed.
Rotavirus A has been detected in a wide variety of mammals and birds. In mammals, both endemic and epidemic forms of rotavirus B, C, E, and H infections have been described, whereas rotavirus D, F, and G have been identified only in birds (1–3). Genetically diverse rotaviruses have been found in some viral metagenomics studies (4,5). Using the metagenomic approach and the VP6-based molecular classification scheme, we found evidence for a novel rotavirus species that we tentatively called Rotavirus I.
Dr. Mihalov-Kovács is a PhD student at the Pathogen Discovery Group, Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences. Her research interests include discovery of novel viruses in domesticated animals.
Financial support was obtained from the Momentum Program (Hungarian Academy of Sciences) and the Hungarian Scientific Research Program (OTKA [Országos Tudományos Kutatási Alapprogramok] 108793; licensing of the Schrödinger Suite software package). Á.G. received a János Bolyai fellowship; F.J. received additional funding from TÁMOP (4.2.4.A/2-11-1-2012-0001).
- Estes MK, Kapikian AZ. Rotaviruses. In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, et al., editors. Fields virology. 5th ed. Philadelphia: Lippincott Williams & Wilkins; 2007. p. 1917–74.
- Matthijnssens J, Otto PH, Ciarlet M, Desselberger U, Van Ranst M, Johne R. VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Arch Virol. 2012;157:1177–82. DOI
- Marthaler D, Rossow K, Culhane M, Goyal S, Collins J, Matthijnssens J, Widespread rotavirus H in commercially raised pigs, United States. Emerg Infect Dis. 2014;20:1195–8 . DOI
- Ng TF, Mesquita JR, Nascimento MS, Kondov NO, Wong W, Reuter G, Feline fecal virome reveals novel and prevalent enteric viruses. Vet Microbiol.2014;171:102–11. DOI
- Li L, Shan T, Wang C, Côté C, Kolman J, Onions D, The fecal viral flora of California sea lions. J Virol. 2011;85:9909–17. DOI
- Kindler E, Trojnar E, Heckel G, Otto PH, Johne R. Analysis of rotavirus species diversity and evolution including the newly determined full-length genome sequences of rotavirus F and G. Infect Genet Evol. 2013;14:58–67. DOI
- Trojnar E, Otto P, Roth B, Reetz J, Johne R. The genome segments of a group D rotavirus possess group A–like conserved termini but encode group-specific proteins. J Virol. 2010;84:10254–65. DOI
- Roy A, Kucukural A, Zhang Y. I-TASSER: a unified platform for automated protein structure and function prediction. Nat Protoc. 2010;5:725–38.DOI
- Mathieu M, Petitpas I, Navaza J, Lepault J, Kohli E, Pothier P, Atomic structure of the major capsid protein of rotavirus: implications for the architecture of the virion. EMBO J. 2001;20:1485–97. DOI
- Matthijnssens J, Ciarlet M, McDonald SM, Attoui H, Bányai K, Brister JR, Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol. 2011;156:1397–413. DOI
- Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald SM, Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1–like and bovine rotavirus strains. J Virol. 2008;82:3204–19 and. DOI
- Marthaler D, Rossow K, Gramer M, Collins J, Goyal S, Tsunemitsu H, Detection of substantial porcine group B rotavirus genetic diversity in the United States, resulting in a modified classification proposal for G genotypes. Virology. 2012;433:85–96. DOI
- Wakuda M, Ide T, Sasaki J, Komoto S, Ishii J, Sanekata T, Porcine rotavirus closely related to novel group of human rotaviruses. Emerg Infect Dis.2011;17:1491–3 .
- Esona MD, Mijatovic-Rustempasic S, Conrardy C, Tong S, Kuzmin IV, Agwanda B, Reassortant group A rotavirus from straw-colored fruit bat (Eidolon helvum). Emerg Infect Dis. 2010;16:1844–52.
Suggested citation for this article: Mihalov-Kovács E, Gellért Á, Marton S, Farkas SL, Fehér E, Oldal M, et al. Candidate new rotavirus species in sheltered dogs, Hungary. Emerg Infect Dis [Internet]. 2015 Apr [date cited]. http://dx.doi.org/10.3201/eid2104.141370
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