Leishmania (Viannia) guyanensis Infection, Austria

To the Editor: Infection with Leishmania spp. was diagnosed in an asymptomatic soldier during an explorative national cross-sectional serologic screening of soldiers volunteering for United Nations missions at the Military Hospital Vienna in 2009. Diagnosis was made by using a commercial ELISA kit (Ridascreen Leishmania; R-Biopharm, Darmstadt, Germany). One year later, the soldier was reassessed for persisting antibodies by using the same ELISA and for Leishmania DNA in a blood sample stored in EDTA by using the Leishmania OligoC-Test (Coris BioConcept, Gembloux, Belgium). (The study was approved by the Research Ethics Committee of the Austrian Armed Forces and written informed consent was obtained from the person investigated.) Because the results of both tests were positive, an additional PCR was performed for identification below genus level with the LITSR/L5.8S primer pair (1). To confirm the PCR results, we sequenced the amplicon in both directions in 2 independent setups and compared the obtained 299-bp sequence with published sequences from GenBank by performing a multiple sequence alignment. Our sequence (strain EN10) showed 100% (299/299 bp) identity with several strains from the Leishmania (Viannia) guyanensis complex, including the L. guyanensis strain MHOM/SR/87/TRUUS4 and the L. panamensis strains FJ948438, FJ948439, and FJ948446. Sequence homology to representatives of the L. (V.) braziliensis complex was ≈93%; to representatives of the L. (Leishmania) mexicana complex, 61%–68%; and to the L. (L.) donovani complex, 70%–71%. The L. (V.) guyanensis complex traditionally includes the species L. guyanensis, L. panamensis, and L. shawi, but L. panamensis seems to be a subspecies or even a synonym of L. guyanensis (2). We thus classified our strain as L. guyanensis. Sequence data were deposited at GenBank (accession no. JN671917).
L. guyanesis/panamensis is found in 9 countries in Central and South America (3). It is a common cause of zoonotic cutaneous leishmaniasis in humans. The sloths Choloepus didactylus (L. guyanensis) and C. hoffmanni (L. panamensis) are believed to be the principal reservoir hosts and the sandfly species Lutzomyia umbratilis (L. guyanensis) and Lu. trapidoi (L. panamensis) to be the principal vectors (3). Also, dogs can act as reservoirs for the L. (V.) guyanensis complex (4).
The infected soldier had never been to Central or South America and had no history of blood transfusions. His lifetime travel history included Italy, Spain, Greece, Germany, Croatia, New York City, and military assignments in Kosovo. Thus, how and where the infection had been acquired remain open for discussion.
Although sandflies are not as robust as Anopheles spp., for example, the most plausible scenario is that either an L. guyanensis–infected sandfly or a noninfected but transmissive sandfly from a disease-endemic area was transported in a ship or airplane (comparable to the well-known “airport malaria” situation) to an area where the patient had traveled. In recent years, Lu. vexator has become widespread and abundant in upstate New York (5). Although, this is not a known vector for L. guyanensis, its spread in New York State shows that Lutzomyia spp. can rapidly adapt to new and distant areas. Of the areas where the infected person had traveled, at least in New York City and Spain, regular introduction of L. guyanensis by immigrants, travelers, or dogs from Central and South America is very likely. Moreover, Leishmania parasites are known to remain viable for a lengthy period in infected humans and animals and even in those that have received treatment.
However, alternative scenarios with other sandfly species, possibly even those found in Europe, acting as vectors cannot be totally excluded. Approximately 25 sandfly species are found in Europe, of which at least 6 are vectors for Leishmania spp (6). Whether L. guyanensis can be transmitted by Phlebotomus sandfly species is unknown. When L. infantum, originally transmitted by Phlebotomus spp., was introduced from Europe to Central and South America in the post-Columbian era, it readily adapted to several vectors of the genus Lutzomyia (3). Adoption of new reservoir hosts and new vector species has also been observed in other species (7). Members of the Leishmania subgenus develop in the midgut, and representatives of the Viannia subgenus develop in the hindgut and the midgut. Nevertheless, several Lutzomyia species can transmit both, representatives of the Viannia and Leishmania subgenera. In general, most sandflies appear to be vector competent for >1 Leishmania spp. The New World species Lu. longipalpis and the Old World species Ph. argentipes, Ph. arabicus, Ph. halepensis, and Ph. perniciosus enabled the maturation of almost all Leishmania species tested under experimental conditions (8). The presence of sandflies in Austria was reported very recently (9), but the vector competence of the species found (P. mascittii) has still not been elucidated. Moreover, this finding likely reflects an increased population density rather than an introduction of a previously nonendemic species.
Nonvector transmission is also a possibility. The infected person did not remember ever having received blood products; however, transmission is generally possible by all forms of blood contact, including through needle sharing among persons who use injection drugs and through sexual intercourse (10).

Wolfgang Poeppl, Heinz Burgmann, Herbert Auer, Gerhard Mooseder, and Julia Walochnik

Author affiliations: Military Hospital Vienna, Vienna, Austria (W. Poeppl, G. Mooseder); and Medical University of Vienna, Vienna (W. Poeppl, H. Burgmann, H. Auer, J. Walochnik)

Acknowledgments

The authors declare that there is no conflict of interest and that all experiments performed comply with the currents laws of Austria.
The study was supported by the Medical University of Vienna and by the Military Hospital Vienna, Austria. Results of the study were presented at the 51st Interscience Conference on Antimicrobial Agents and Chemotherapy, September 17–20, 2011, Chicago, Illinois, USA (abstract P-929).

References

El Tai NO, Osman OF, El Fari M, Presber W, Schonian G. Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing. Trans R Soc Trop Med Hyg. 2000;94:575–9. DOI PubMed
Schönian G, Mauricio I, Cupolillo E. Is it time to revise the nomenclature of Leishmania? Trends Parasitol. 2010;26:466–9. DOI PubMed
Lainson R, Shaw JJ. New World leishmaniasis. In: Cox F, Wakelin D, Gillespie S, Despommier D, editors. Topley and Wilson’s microbiology and microbial infections—parasitology. 10th ed. Chichester (UK): John Wiley & Sons; 2005.
Santaella J, Ocampo CB, Saravia NG, Mendez F, Gongora R, Gomez MA, Leishmania (Viannia) infection in the domestic dog in Chaparral, Colombia. Am J Trop Med Hyg. 2011;84:674–80. DOI PubMed
Ostfeld RS, Roy P, Haumaier W, Canter L, Keesing F, Rowton ED. Sand fly (Lutzomyia vexator) (Diptera: Psychodidae) populations in upstate New York: abundance, microhabitat, and phenology. J Med Entomol. 2004;41:774–8. DOI PubMed
Killick-Kendrick R. The biology and control of phlebotomine sand flies. Clin Dermatol. 1999;17:279–89. DOI PubMed
Ashford RW. The leishmaniases as emerging and reemerging zoonoses. Int J Parasitol. 2000;30:1269–81. DOI PubMed
Wilson R, Bates MD, Dostalova A, Jecna L, Dillon RJ, Volf P, Stage-specific adhesion of Leishmania promastigotes to sand fly midguts assessed using an improved comparative binding assay. PLoS Negl Trop Dis. 2010;4:e816. DOI PubMed
Naucke TJ, Lorentz S, Rauchenwald F, Aspock H. Phlebotomus (Transphlebotomus) mascittii Grassi, 1908, in Carinthia: first record of the occurrence of sandflies in Austria (Diptera: Psychodidae: Phlebotominae). Parasitol Res. 2011;109:1161–4. DOI PubMed
Symmers WS. Leishmaniasis acquired by contagion: a case of marital infection in Britain. Lancet. 1960;1:127–32.PubMed

Suggested citation for this article: Poeppl W, Burgmann H, Auer H, Mooseder G, Walochnik J. Leishmania (Viannia) guyanensis infection, Austria [letter]. Emerg Infect Dis [serial on the Internet]. 2012 Sep [date cited]. http://dx.doi.org/10.3201/eid1809.111365