A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis.

Wan YW, Raese RA, Fortney JE, Xiao C, Luo D, Cavendish J, Gibson LF, Castranova V, Qian Y, Guo NL.

Source

Mary Randolph Cancer Center, West Virginia University, Morgantown, WV 26506, USA.

Abstract

Smoking is responsible for 90% of lung cancer cases. There is currently no clinically available gene test for early detection of lung cancer in smokers, or an effective patient selection strategy for adjuvant chemotherapy in lung cancer treatment. In this study, concurrent coexpression with multiple signaling pathways was modeled among a set of genes associated with smoking and lung cancer survival. This approach identified and validated a 7-gene signature for lung cancer diagnosis and prognosis in smokers using patient transcriptional profiles (n=847). The smoking-associated gene coexpression networks in lung adenocarcinoma tumors (n=442) were highly significant in terms of biological relevance (network precision = 0.91, FDR<0 .01=".01" 4-="4-" adenocarcinoma="adenocarcinoma" and="and" assays="assays" associations.="associations." biomarkers="biomarkers" blotting="blotting" butanone="butanone" cancer="cancer" carcinogenesis="carcinogenesis" cells="cells" coexpression="coexpression" confirmed="confirmed" containing="containing" databases="databases" egfr="egfr" epithelial="epithelial" evaluated="evaluated" functional="functional" furthermore="furthermore" gene="gene" genes="genes" h23="h23" human="human" identified="identified" in="in" involved="involved" involvement="involvement" lung="lung" methylnitrosamino="methylnitrosamino" molecular="molecular" multi-level="multi-level" network="network" nnk-treated="nnk-treated" normal="normal" numerous="numerous" of="of" p53="p53" pathway="pathway" patients="patients" phospho="phospho" progression.="progression." pyridyl="pyridyl" qrt-pcr="qrt-pcr" rb="rb" results="results" signaling="signaling" smoking-induced="smoking-induced" smoking="smoking" support="support" the="the" their="their" transformed="transformed" treated="treated" was="was" western="western" when="when" with="with">