Tracking sex-dependent differences in a mouse model of CLN6-Batten disease

McKayla J. Poppens†,
Jacob T. Cain†,
Tyler B. Johnson,
Katherine A. White,
Samantha S. Davis,
Rachel Laufmann,
Alexander D. Kloth and
Jill M. WeimerEmail author View ORCID ID profile

†Contributed equally

Orphanet Journal of Rare Diseases201914:19

https://doi.org/10.1186/s13023-019-0994-8

Received: 21 July 2018
Accepted: 7 January 2019
Published: 21 January 2019

Abstract

Background

CLN6-Batten disease is a rare neurodevelopmental disorder characterized pathologically by the accumulation of lysosomal storage material, glial activation and neurodegeneration, and phenotypically by loss of vision, motor coordination, and cognitive ability, with premature death occurring in the second decade of life. In this study, we investigate whether sex differences in a mouse model of CLN6-Batten disease impact disease onset and progression.

Results

A number of noteworthy differences were observed including elevated accumulation of mitochondrial ATP synthase subunit C in the thalamus and cortex of female Cln6 mutant mice at 2 months of age. Moreover, female mutant mice showed more severe behavioral deficits. Beginning at 9 months of age, female mice demonstrated learning and memory deficits and suffered a more severe decline in motor coordination. Further, compared to their male counterparts, female animals succumbed to the disease at a slightly younger age, indicating an accelerated disease progression. Conversely, males showed a marked increase in microglial activation at 6 months of age in the cortex relative to females.

Conclusions

Thus, as female Cln6 mutant mice exhibit cellular and behavioral deficits that precede similar pathologies in male mutant mice, our findings suggest the need for consideration of sex-based differences in CLN6 disease progression during development of preclinical and clinical studies.