CIENCIASMEDICASNEWS: PBS-only RabMAb® antibodies for easier assay development

miércoles, 23 de enero de 2019

PBS-only RabMAb® antibodies for easier assay development | Abcam

	January 23, 2019
	Antibodies
	The latest antibodies news from News Medical

Reformulated Recombinant Antibodiesfor Mass Cytometry and Multiplex Analysis

The simultaneous detection of multiple antigens can be used to understand ever more complex biological problems. Techniques such as imaging mass cytometry (CyTOF®) and multiplex IHC require antibody labelling to metals or fluorochromes. Abcam’s reformulated recombinant RabMAb antibodies allow for easy conjugation to use in these functional assays.

Lab-Scale Clarification of Mammalian Cell Cultures Expressing Recombinant Antibodies

Tina Stoschek and Marcus Gerlach discuss maximizing mAb generation using lab-scale clarification of cell cultures expressing recombinant antibodies.

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Uniquely Designed Fusion Proteins for Llama Immunization

In 1993, the single domain antibody was first discovered in camels. This new category of antibody is heavy chain only, containing a single variable domain (VHH) and two constant domains (CH2, CH3). This article will outline the advantages of this unusual form of antibody and explore how the Llama IgG Fc protein is an interesting alternative to traditional antibodies when it comes to therapeutic use.

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	Researchers Use DNA Origami to Enhance Effectiveness of Antibodies Used in Vaccines and Immunotherapy Researchers from Karolinska Institutet, in collaboration with scientists at the University of Oslo, have used DNA origami—DNA-based design of precise nanostructures—and demonstrated the most accurate length between densely packed antigens to obtain the strongest bond to antibodies in the immune system.

	Genetic Techniques of Antibody Validation Much time and effort are expended on antibody studies, but these can only produce successful and reproducible outcomes if the antibody used is specific. Antibody specificity refers to whether the molecule is capable of binding the target consistently with minimal or no cross-reactivity.

	Difference Between a Paratope and an Epitope Paratopes and epitopes are the unique binding regions of an antibody and antigen, respectively. More specifically, antigens are known to contain specific antigenic determinants (which are epitopes), while antibodies contain antigen-binding sites (which are paratopes).

	Types of Antibodies Antibodies are glycoproteins, termed as immunoglobulins (Igs), which are produced in response to an immune reaction and specifically bind to antigens responsible for initiating the reaction.

	How are Antibodies Validated? If an antibody is not specific or selective enough for its application, it will bind to several different epitopes, thereby reducing its accuracy and costing the laboratory both time and money. Accuracy is also affected if antibodies from the same manufacturer or batch do not generate reproducible results.

	How Should Antibodies be Stored? Each antibody has a different optimal storage environment, ensuring their stability and reducing their degradation. If stored incorrectly, the survival of the antibody can be dramatically reduced, lasting only a few weeks rather than a few years. This article will discuss some of the key factors to consider when selecting a storage environment for a specific antibody.