BMC Molecular Biology
RESEARCH ARTICLE
OPEN ACCESS
Gene expression profiling of the venom gland from the Venezuelan mapanare (Bothrops colombiensis) using expressed sequence tags (ESTs)
BMC Molecular Biology201617:7
DOI: 10.1186/s12867-016-0059-7
© Suntravat et al. 2016
Received: 14 September 2015
Accepted: 23 February 2016
Published: 5 March 2016
Abstract
Background
Bothrops colombiensis is a highly dangerous pit viper and responsible for over 70 % of snakebites in Venezuela. Although the composition in B. colombiensis venom has been identified using a proteome analysis, the venom gland transcriptome is currently lacking.
Results
We constructed a cDNA library from the venom gland of B. colombiensis, and a set of 729 high quality expressed sequence tags (ESTs) was identified. A total number of 344 ESTs (47.2 % of total ESTs) was related to toxins. The most abundant toxin transcripts were metalloproteinases (37.5 %), phospholipases A2s (PLA2, 29.7 %), and serine proteinases (11.9 %). Minor toxin transcripts were linked to waprins (5.5 %), C-type lectins (4.1 %), ATPases (2.9 %), cysteine-rich secretory proteins (CRISP, 2.3 %), snake venom vascular endothelium growth factors (svVEGF, 2.3 %), L-amino acid oxidases (2 %), and other putative toxins (1.7 %). While 160 ESTs (22 % of total ESTs) coded for translation proteins, regulatory proteins, ribosomal proteins, elongation factors, release factors, metabolic proteins, and immune response proteins. Other proteins detected in the transcriptome (87 ESTs, 11.9 % of total ESTs) were undescribed proteins with unknown functions. The remaining 138 (18.9 %) cDNAs had no match with known GenBank accessions.
Conclusion
This study represents the analysis of transcript expressions and provides a physical resource of unique genes for further study of gene function and the development of novel molecules for medical applications.
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