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Detection of Circovirus in Foxes with Meningoencephalitis, United Kingdom, 2009–2013 - Volume 21, Number 7—July 2015 - Emerging Infectious Disease journal - CDC

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Detection of Circovirus in Foxes with Meningoencephalitis, United Kingdom, 2009–2013 - Volume 21, Number 7—July 2015 - Emerging Infectious Disease journal - CDC





Volume 21, Number 7—July 2015

Dispatch

Detection of Circovirus in Foxes with Meningoencephalitis, United Kingdom, 2009–2013

Steve Bexton, Lidewij C. Wiersma, Sarah Getu, Peter R. van Run, Georges M.G.M. Verjans, Debby Schipper, Claudia M.E. Schapendonk, Rogier Bodewes, Lucy Oldroyd, Bart L. Haagmans, Marion M.P. Koopmans, and Saskia L. SmitsComments to Author 
Author affiliations: RSPCA Norfolk Wildlife Hospital, East Winch, United Kingdom (S. Bexton)Erasmus Medical Center, Rotterdam, the Netherlands (L.C. Wiersma, S. Getu, P.R. van Run, G.M.G.M. Verjans, D. Schipper, C.M.E. Schapendonk, R. Bodewes, B.L. Haagmans, M.M.P. Koopmans, S.L. Smits)Abbey Veterinary Services, Newton Abbot, United Kingdom (L. Oldroyd)National Institute for Public Health and the Environment, Bilthoven, the Netherlands (M.M.P. Koopmans)

Abstract

A fox circovirus was identified in serum samples from foxes with unexplained neurologic signs by using viral metagenomics. Fox circovirus nucleic acid was localized in histological lesions of the cerebrum by in situ hybridization. Viruses from the family Circoviridae may have neurologic tropism more commonly than previously anticipated.
Circoviruses (family Circoviridae) are nonenveloped, single-stranded, circular DNA (≈2 kb) viruses (1). Two genera, Circovirus and Gyrovirus, are recognized, and an additional genus, Cyclovirus, has been proposed (1,2). Circoviruses have an ambisense genome organization with 2 major inversely arranged open reading frames encoding the rolling circle replication initiator protein gene (Rep) and a capsid protein gene (Cap) (1). A conserved stem–loop structure, required for viral replication, is located between the 5′ ends of the 2 main open reading frames. Circoviruses are thought to exhibit host species specificity and have been detected in various species, including birds, pigs, and dogs (1,3,4). These viruses have been associated with a variety of diseases, including respiratory and enteric disease, dermatitis, and reproductive problems (1,35). Recently, many small circular DNA genomes have been described from different hosts by using different methods, including high-throughput sequencing (6). Here we describe the identification, characterization, and prevalence of a newly discovered fox circovirus that was present in serum and brain samples from foxes with unexplained meningoencephalitis in the United Kingdom.
Mr. Bexton is the senior veterinarian at the RSPCA Norfolk Wildlife Hospital, East Winch, United Kingdom. His main research interests are the epidemiology and pathology of wildlife diseases including novel pathogens in free-living animals.

Acknowledgment

This work was partially funded by the European Commission’s COMPARE H2020 project under grant agreement No 643476, the Virgo Consortium, and ZonMW TOP project 91213058.

References

  1. Todd DBendinelli MBiagini PHino SMankertz AMishiro SCircoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, editors. Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses. San Diego: Elsevier Academic Press; 2005. p. 327–34.
  2. Li LKapoor ASlikas BBamidele OSWang CShaukat SMultiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces. J Virol2010;84:167482DOIPubMed
  3. Li LMcGraw SZhu KLeutenegger CMMarks SLKubiski SCircovirus in tissues of dogs with vasculitis and hemorrhage. Emerg Infect Dis.2013;19:53441DOIPubMed
  4. Todd DAvian circovirus diseases: lessons for the study of PMWS. Vet Microbiol2004;98:16974DOIPubMed
  5. Opriessnig THalbur PGConcurrent infections are important for expression of porcine circovirus associated disease. Virus Res2012;164:2032.DOIPubMed
  6. Delwart ELi LRapidly expanding genetic diversity and host range of the Circoviridae viral family and other Rep encoding small circular ssDNA genomes. Virus Res2012;164:11421DOIPubMed
  7. Bodewes Rvan der Giessen JHaagmans BLOsterhaus ADSmits SLIdentification of multiple novel viruses, including a parvovirus and a hepevirus, in feces of red foxes. J Virol2013;87:775864DOIPubMed
  8. van den Brand JMvan Leeuwen MSchapendonk CMSimon JHHaagmans BLOsterhaus ADMetagenomic analysis of the viral flora of pine marten and European badger feces. J Virol2012;86:23605DOIPubMed
  9. van Leeuwen MWilliams MMKoraka PSimon JHSmits SLOsterhaus ADHuman picobirnaviruses identified by molecular screening of diarrhea samples. J Clin Microbiol2010;48:178794DOIPubMed
  10. Schürch ACSchipper DBijl MADau JBeckmen KBSchapendonk CMMetagenomic survey for viruses in Western Arctic caribou, Alaska, through iterative assembly of taxonomic units. PLoS ONE2014;9:e105227.DOIPubMed
  11. Smits SLZijlstra EEvan Hellemond JJSchapendonk CMBodewes RSchürch ACNovel cyclovirus in human cerebrospinal fluid, Malawi, 2010–2011. Emerg Infect Dis2013;19:1511-3.DOIPubMed
  12. Prachayangprecha SSchapendonk CMKoopmans MPOsterhaus ADSchürch ACPas SDExploring the potential of next-generation sequencing in detection of respiratory viruses. J Clin Microbiol2014;52:372230DOIPubMed
  13. Kapoor ADubovi EJHenriquez-Rivera JALipkin WIComplete genome sequence of the first canine circovirus. J Virol2012;86:7018.DOIPubMed
  14. Rose NOpriessnig TGrasland BJestin AEpidemiology and transmission of porcine circovirus type 2 (PCV2). Virus Res2012;164:7889.DOIPubMed
  15. Tan LVvan Doorn HRNghia HDChau TTTu LTde Vries MIdentification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections. MBio2013;4:e0023113DOIPubMed

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Technical Appendix

Suggested citation for this article: Bexton S, Wiersma LC, Getu S, van Run PR, Verjans GM, Schipper D, et al. Detection of circovirus in foxes with meningoencephalitis, United Kingdom. Emerg Infect Dis. 2015 Jul [date cited]. http://dx.doi.org/10.3201/eid2107.150228
DOI: 10.3201/eid2107.150228

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