CREST maps somatic structural variation in cancer ... [Nat Methods. 2011] - PubMed result

Nat Methods. 2011 Jun 12. doi: 10.1038/nmeth.1628. [Epub ahead of print]
CREST maps somatic structural variation in cancer genomes with base-pair resolution.
Wang J, Mullighan CG, Easton J, Roberts S, Heatley SL, Ma J, Rusch MC, Chen K, Harris CC, Ding L, Holmfeldt L, Payne-Turner D, Fan X, Wei L, Zhao D, Obenauer JC, Naeve C, Mardis ER, Wilson RK, Downing JR, Zhang J.

Source
Department of Information Sciences, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Abstract
We developed 'clipping reveals structure' (CREST), an algorithm that uses next-generation sequencing reads with partial alignments to a reference genome to directly map structural variations at the nucleotide level of resolution. Application of CREST to whole-genome sequencing data from five pediatric T-lineage acute lymphoblastic leukemias (T-ALLs) and a human melanoma cell line, COLO-829, identified 160 somatic structural variations. Experimental validation exceeded 80%, demonstrating that CREST had a high predictive accuracy.

PMID:21666668[PubMed - as supplied by publisher]
CREST maps somatic structural variation in cancer ... [Nat Methods. 2011] - PubMed result