domingo, 31 de mayo de 2020

Rapid and Inexpensive Whole-Genome Sequencing of SARS-CoV2 using 1200 bp Tiled Amplicons and Oxford Nanopore Rapid Barcoding NE Freed et al, BIORXIV, May 29, 2020

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Coronavirus

Rapid and cost-efficient whole-genome sequencing of SARS-CoV-2, the virus that causes COVID-19, is critical for understanding viral transmission dynamics. Here we show that using a new multiplexed set of primers in conjunction with the Oxford Nanopore Rapid Barcode library kit allows for faster, simpler, and less expensive SARS-CoV-2 genome sequencing.
The study sought to develop and externally validate COVID-19 Estimated Risk (COVER) scores that quantify a patient's risk of hospital admission, requiring intensive services, or fatality in the 30-days following diagnosis. The design was a multinational, distributed network cohorts from 13 data sources and 6 countries, mapped to a common data model.
Combining PCR and CT testing for COVID
C Shen et al, MEDRXIV, May 29, 2020

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