Robust sampling and preservation of DNA for microbial community profiling in field experiments

Anneloes E. GroenenboomEmail author,
Eddy J. Smid and
Sijmen E. Schoustra

BMC Research Notes201912:159

https://doi.org/10.1186/s13104-019-4187-2

Received: 30 January 2019
Accepted: 13 March 2019
Published: 22 March 2019

Abstract

Objective

Stabilising samples of microbial communities for DNA extraction without access to laboratory equipment can be a challenging task. In this paper we propose a method using filter paper disks for the preservation of DNA from diverse microbial communities which are found in a fermented milk product.

Results

Small adaptations to the DNA extraction method used for liquid fermented milk delivered DNA of sufficient amounts and quality to be used for later analyses, e.g. full community 16S amplicon sequencing. The microbial community structure obtained via the filter paper method showed sufficient resemblance to the structure obtain via the traditional DNA extraction from the liquid milk sample. This method can therefore successfully be used to analyse diverse microbial communities from fermented milk products from remote areas.