CIENCIASMEDICASNEWS: Monitoring Intracellular Calcium Using Fluorescent Dyes in a Mid-throughput Assay

viernes, 29 de marzo de 2019

Monitoring Intracellular Calcium Using Fluorescent Dyes in a Mid-throughput Assay

	March 29, 2019
	Fluorescence
	The latest fluorescence news from AZoNetwork

Monitoring Intracellular Calcium Using Fluorescent Dyes in a Mid-throughput Assay
Which dye is suited best for monitoring intracellular Ca2+ in a microplate reader? We compared three Ca2+ dyes regarding their response to receptor activation and undesired leakage out of the cell.

Characterisation of Phosphorescent Organic Light Emitting Diode (PhOLED) Using Photoluminescence and Electroluminescence Spectroscopy

Daily experiences with organic (carbon based) materials, for example polyvinyl chloride insulation on cables and polyethylene shopping bags suggests that they should be electrical insulators. This is true for the majority of organic materials however there are a small subset of organic materials with a particular electronic structure that are semiconducting and so can be used to make optoelectronic devices, such as light emitting diodes.

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Avoiding Cell Death in Fluorescence Microscopy

Fluorescence microscopy is a common laboratory tool used to investigate the physiology of different cells. Specific wavelengths of light are shone onto cells in a sample, where the light is absorbed by molecules called fluorophores.

	E-FRET Microscopy for Live Cell Imaging E-FRET microscopy recently became a very popular intensity-based Fluorescence Resonance Energy Transfer (FRET) method for cell quantification. This technique reduces the photobleaching observed during the process of FRET.

	Fluorescence Quenching Fluorescence quenching is a physicochemical process that lowers the intensity of emitted light from fluorescent molecules. When a molecule absorbs light, electrons in its constituent atoms become excited and are promoted to a higher energy level. When electrons in this excited state lose energy and return to the ground state, they release this energy in the form of heat or radiation.

	Portable mini microscope could improve scientists' ability to study brain disease in living mice Working with mice, a team of Johns Hopkins Medicine researchers has developed a relatively inexpensive, portable mini microscope that could improve scientists' ability to image the effects of cancer, stroke, Alzheimer's disease and other conditions in the brains of living and active mice over time.

	Researchers Use a Single Fluorophore to Simultaneously Image Different Molecules In day-to-day life, blinking lights can be used for sending signals––for instance, that a car is about to turn. At present, tiny “blinkers” that show single molecules of RNA or protein within cells based on the duration and frequency of every flash have been devised by scientists.