Human Endophthalmitis Caused By Pseudorabies Virus Infection, China, 2017

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Jing-Wen Ai1, Shan-Shan Weng1, Qi Cheng, Peng Cui, Yong-Jun Li, Hong-Long Wu, Yi-Min Zhu, Bin Xu

, and Wen-Hong Zhang

Author affiliations: Huashan Hospital of Fudan University, Shanghai, China (J.-W. Ai, S.-S. Weng, Q. Cheng, P. Cui, Y.-M. Zhu, B. Xu, W.-H. Zhang); BGI genomics, BGI-Shenzen, Shenzen, China (Y.-J. Li); Binhai Genomics Institute, Translational Genomics Center, Tianjin, China (H.-L. Wu)

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Abstract

We report human endophthalmitis caused by pseudorabies virus infection after exposure to sewage on a hog farm in China. High-throughput sequencing and real-time PCR of vitreous humor showed pseudorabies virus sequences. This case showed that pseudorabies virus might infect humans after direct contact with contaminants.

Pseudorabies virus (PRV) primarily infects swine and has several secondary hosts, including cattle, dogs, and cats. PRV, also called Aujeszky disease virus or Suid herpesvirus 1, is a member of the Alphaherpesvirinae subfamily within the family Herpesviridae. PRV infection has not been confirmed in humans (1), but previous reports have suggested the possible presence of PRV infection in 3 immunocompetent humans in whom fever, sweating, and neurologic complaints developed; virus neutralization and immune precipitation tests were positive for PRV antibody (2,3). We report a human case of human infectious endophthalmitis caused by PRV in a woman from Jiangxi Province, China, in July 2017.

The Study

On June 14, 2017, sewage from a hog farm spilled onto a 46-year-old woman from Jiangxi Province, China, who worked as a swineherder; her daily work was to feed swine and clean hoggery sewage.. The next day, she had a headache and fever of 39.5°C. Three days later, she became visually impaired and was admitted to a local hospital, where she was treated empirically with meropenem, vancomycin, and acyclovir. On June 29, after no significant improvement, she was transferred to Huashan Hospital of Fudan University (Shanghai, China), to treat unresponsive fever, headaches, and visual impairment.

Thumbnail of Ocular examination conducted 2 months after eye surgery in patient with human endophthalmitis caused by pseudorabies virus, China, 2017. A) Fundus photography of both eyes showing retinal necrosis and occlusive vasculitis and postoperative change after pars plana vitrectomy with silicone oil injection in the right eye. B) Optical coherence tomography of the patient’s right eye showed postoperative change after pars plana vitrectomy. 2, scan depth (2 mm); I, inferior, L, left eye; N,

Figure 1. Ocular examination conducted 2 months after eye surgery in patient with human endophthalmitis caused by pseudorabies virus, China, 2017. A) Fundus photography of both eyes showing retinal necrosis and occlusive vasculitis...

On examination, she had palpebral conjunctival congestion, and visual acuity to light perception of both eyes had worsened. Slit-lamp examination showed keratic precipitates and Tyndall effect flare. Funduscopic examination revealed vitreous opacity (Figure 1, panel A) and a pale white lesion on the posterior pole of the right eye (Figure 1, panel B), which suggested acute retinal necrosis and occlusive vasculitis. Results of routine laboratory testing were normal, including serology tests for HIV and hepatitis B and C, T-SPOT.TB test (Oxford Immunotec Ltd., Oxford, UK), blood culture, cryptococcal latex agglutination test, autoantibodies, and cerebrospinal fluid test. Test results for plasma Epstein-Barr virus and cytomegalovirus (CMV) IgG were positive; IgM was negative for both pathogens.

The on-staff ophthalmologist diagnosed endophthalmitis in the patient, considering viral infection as most likely. The patient was transferred to the ophthalmology department for vitrectomy surgery on the right eye on June 30. During the operation, ≈2 mL of vitreous humor was taken for culture and next-generation sequencing (NGS) (Technical Appendix[PDF - 553 KB - 8 pages])

Thumbnail of Results of gene sequencing, serologic testing, and phylogenetic analysis of pseudorabies virus from patient with human endophthalmitis, China, 2017. A) Sequencing of Suid herpesvirus 1 (pseudorabies virus) yielded a total coverage of 84%. B) Evolutionary relationships of taxa. Phylogenetic analysis disclosed a close connection between the isolate from the patient (boldface) and 4 other Suid herpesvirus 1 strains. Scale bar indicates amino acid substitutions per site.

Figure 2. Results of gene sequencing, serologic testing, and phylogenetic analysis of pseudorabies virus from patient with human endophthalmitis, China, 2017. A) Sequencing of Suid herpesvirus 1 (pseudorabies virus) yielded a total coverage...

On July 2, NGS results showed 4,832 unique sequence reads of PRV in vitreous humor, covering 84% of the nucleotide sequences (Table; Figure 2, panel A); NGS results for cerebrospinal fluid (CSF) were negative for PRV. Other detected sequences were within laboratory reference ranges. On the basis of NGS results, the physicians, who suspected that the patient may have acquired PRV infection, immediately initiated valacyclovir therapy. Sanger sequencing (Technical Appendix[PDF - 553 KB - 8 pages] Figure 1) and PCR analysis (Technical Appendix[PDF - 553 KB - 8 pages] Figures 2, 3) confirmed identification of PRV in vitreous humor (4–6). Phylogenetic analysis disclosed a close connection between the isolated strain and 3 emergent and highly pathogenic PRV variants in China (Figure 2, panel B; Technical Appendix[PDF - 553 KB - 8 pages]) (7,8).

To further validate our results, we constructed a plasmid and a standard curve for real-time PCR and obtained quantitative results of viral DNA load as 2.7 × 106 copies/mL (Technical Appendix[PDF - 553 KB - 8 pages] Figure 3). To rule out contamination, we performed PCR of the PRV on a control group of 7 persons; all were negative (Technical Appendix[PDF - 553 KB - 8 pages] Figure 2). Other possible viral causes of infective endophthalmitis, such as varicella-zoster virus, herpes simplex virus, and cytomegalovirus, were excluded through PCR. One week after surgery, culture result for vitreous humor was negative; the patient’s fever and headache had resolved, and visual acuity had improved slightly. The patient was discharged on July 11; during the last clinical check-up (December 6, 2017), the visual acuity of her left eye had improved to 0.2, and the right eye remained at slight light perception.

During the follow-up period, we obtained the patient’s plasma and CSF samples and ordered PRV antibody testing. PRV antibody was detected in all plasma samples at 4 and 5 months after disease onset and in CSF samples at 2 weeks to 2 months after disease onset, indicating the patient’s previous contact with PRV (Technical Appendix[PDF - 553 KB - 8 pages] Figure 4). Epstein-Barr virus, CMV, and PRV serologic tests were also conducted on control samples and ruled out the possibility of cross-reaction (Technical Appendix[PDF - 553 KB - 8 pages] Table 2) (9,10). Although the patient experienced headache and fever during disease progression and CSF PRV antibody test was positive, routine tests, NGS, and PCR of the CSF all failed to disclose abnormality. Therefore, we do not have enough evidence to suggest possible PRV central nervous system infection. Considering epidemiologic history, clinical symptoms, and serologic and molecular testing results, we diagnosed PRV endophthalmitis.

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Conclusions

The first 2 suspected cases of human PRV infection were reported in 1914, but detection of antibodies or cultivation of the virus had failed. In 1987, Mravak reported 3 suspected cases of human PRV infection with positive serum antibodies (2). All 3 patients were immunocompetent and their clinical manifestation occurred 1–3 weeks after possible animal contact. Initial symptoms included fever, sweating, and weakness; later, central nervous system symptoms developed. Some symptoms persisted for months, and serologic PRV antibodies were positive 5–15 months after the onset of clinical symptoms. In our study, the patient had similar symptoms, plus visual impairment and a unique infection route of PRV through direct exposure to contaminants.

Since 2011, prevalence of PRV infection among swineherds in China has risen several times (11). Among swineherds in Jiangxi Province, studies have shown varying positive rates for PRV DNA: from 5.5% to 26.5% during 2014, (12), and of positive PRV antibodies, from 84.4% to 89.9% during September 2013–September 2015 (13). PRV vaccine is still provided for swineherds on a voluntary basis rather than as a requirement in China, and swine were not vaccinated in the hoggery in which this patient worked at that time. On the day of disease onset, the patient’s eyes were directly contaminated with sewage containing pig excrement when cleaning pig sties. Although no previous study had reported a confirmed case of PRV-caused infectious endophthalmitis, the typical infectious route in this case made hoggery the most probable infectious source in this study.

NGS is marked by its rapid diagnostic ability to precisely identify certain pathogens in peripheral blood, respiratory, and CSF samples. In this case, NGS testing in facilitating the diagnosis of infective endophthalmitis is supported. The fact that real-time PCR and Sanger sequencing results were consistent with the NGS results further validated the credibility of this technique. Furthermore, a serologic test was conducted during the follow-up period; results showed that, even 5 months after symptom onset, the antibodies to PRV in plasma were still active, and the antibodies in CSF persisted during the entire follow-up period (Technical Appendix[PDF - 553 KB - 8 pages] Figure 4). This result is similar to that reported in 1987, indicating that PRV antibodies may persist long after the initial infections.

In summary, this case of PRV-caused human infectious endophthalmitis indicates that PRV could affect humans through direct contact with pig contaminants. NGS of the vitreous humor provided a strong technical support for rapid diagnosis of PRV infection in this patient. However, the pathogenesis of PRV infection remains to be explored. This case stresses the importance of mandatory PRV vaccine among swineherds and the necessity for workers in the breeding industry to increase awareness of self-protection when handling animal containments.

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Dr. Ai is a clinical resident at Huashan Hospital affiliated to Fudan University. Her research interests include the precision medicine of infectious diseases and the use of high throughput sequencing in assisting the rapid diagnosis of causative pathogens.

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Acknowledgments

We thank the patient for cooperating with our investigation and acknowledge the professionalism and compassion demonstrated by all the healthcare workers involved in her care.

This study was supported by the New and Advanced Technology Project of Shanghai Municipal Hospital: Application of next generation sequencing technique in precise diagnosis of infectious diseases (SHDC12017104).

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References

Woźniakowski G, Samorek-Salamonowicz E. Animal herpesviruses and their zoonotic potential for cross-species infection. Ann Agric Environ Med. 2015;22:191–4.
Mravak S, Bienzle U, Feldmeier H, Hampl H, Habermehl KO. Pseudorabies in man. Lancet. 1987;1:501–2.
Michaels MG. Infectious concerns of cross-species transplantation: xenozoonoses. World J Surg. 1997;21:968–74. DOI PubMed
Ma W, Lager KM, Richt JA, Stoffregen WC, Zhou F, Yoon KJ. Development of real-time polymerase chain reaction assays for rapid detection and differentiation of wild-type pseudorabies and gene-deleted vaccine viruses. J Vet Diagn Invest. 2008;20:440–7. DOI PubMed
Wernike K, Beer M, Freuling CM, Klupp B, Mettenleiter TC, Müller T, et al. Molecular double-check strategy for the identification and characterization of Suid herpesvirus 1. J Virol Methods. 2014;209:110–5. DOI PubMed
Nonaka CKV, Fonseca AA Junior, Guedes EO, D’Ambros RM, Lima GK, Camargos MF, et al. Different methods of real-time PCR for detection of pseudorabies virus. Cienc Rural. 2017;47:1–7. DOI
Luo Y, Li N, Cong X, Wang CH, Du M, Li L, et al. Pathogenicity and genomic characterization of a pseudorabies virus variant isolated from Bartha-K61-vaccinated swine population in China.Vet Microbiol. 2014;174:107–15. DOI PubMed
Ye C, Guo JC, Gao JC, Wang TY, Zhao K, Chang XB, et al. Genomic analyses reveal that partial sequence of an earlier pseudorabies virus in China is originated from a Bartha-vaccine-like strain. Virology. 2016;491:56–63. DOI PubMed
Shen Y, Zhang S, Sun R, Wu T, Qian J. Understanding the interplay between host immunity and Epstein-Barr virus in NPC patients. Emerg Microbes Infect. 2015;4:e20. DOI PubMed
Krech U. Complement-fixing antibodies against cytomegalovirus in different parts of the world. Bull World Health Organ. 1973;49:103–6.PubMed
Sun Y, Luo Y, Wang CH, Yuan J, Li N, Song K, et al. Control of swine pseudorabies in China: Opportunities and limitations. Vet Microbiol. 2016;183:119–24. DOI PubMed
Zhang YQ. Molecular epidemiological investigation of CSFV, PRRSV, PCV2 and PRV of nursery pigs in Jiangxi in 2014 [in Chinese]. Jiangxi: Jiangxi Agricultural University; 2016.
Ye FH. 2013–2015 parts of large scale pig farms in Jiangxi province epidemiological investigation of swine pseudorabies [in Chinese]. Jiangxi: Jiangxi Agricultural University; 2016.