sábado, 25 de febrero de 2017

Impact of viral multiplex real-time PCR on management of respiratory tract infection: a retrospective cohort study | Pneumonia | Full Text

Impact of viral multiplex real-time PCR on management of respiratory tract infection: a retrospective cohort study | Pneumonia | Full Text

Biomed Central

Pneumonia

Impact of viral multiplex real-time PCR on management of respiratory tract infection: a retrospective cohort study

  • Lena M. Mayer,
  • Christian Kahlert,
  • Frank Rassouli,
  • Pietro Vernazza and
  • Werner C. AlbrichEmail author
Pneumonia20179:4
DOI: 10.1186/s41479-017-0028-z
Received: 4 October 2016
Accepted: 18 January 2017
Published: 25 February 2017

Abstract

Background

Significance and clinical utility of multiple virus detection by multiplex real-time polymerase chain reaction (rtPCR) in respiratory tract infection remain unclear.

Methods

This retrospective cohort study analyzed how virus detection affected clinical management. During a 27-month period, clinical and laboratory information was collected from all children and adults in two Swiss tertiary centres whose respiratory samples were tested for respiratory viruses with a 16-plex rtPCR test.

Results

Pathogens were identified in 140 of 254 patients (55%); of those patients, there was ≥1 virus in 91 (65%), ≥ 1 bacterium in 53 (38%), and ≥1 virus and bacterium in 11 (8%). Of 80 patients with viral infection, 59 (74%) received antibiotics. Virus detection was associated with discontinuation of antibiotics in 2 of 20 adults (10%) and 6 of 14 children (43%). Overall 12 adults (34%) and 18 children (67%) were managed correctly without antibiotics after virus detection (p = 0.01). When taking biomarkers, radiologic presentations, and antibiotic pre-treatment into account, the impact of rtPCR and appropriateness of therapy for clinically viral infections increased to 100% in children and 62% in adults.

Conclusions

A substantial reduction of unnecessary antibiotic prescriptions seems possible. Appropriate application of rtPCR results in respiratory tract infections should be encouraged.

Keywo

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