Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients. - PubMed - NCBI
J Mol Diagn. 2015 Sep;17(5):605-15. doi: 10.1016/j.jmoldx.2015.04.010.
Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients.
Ramírez JC1,
Cura CI1,
da Cruz Moreira O2,
Lages-Silva E3,
Juiz N1,
Velázquez E4,
Ramírez JD5,
Alberti A6,
Pavia P7,
Flores-Chávez MD8,
Muñoz-Calderón A9,
Pérez-Morales D10,
Santalla J11,
Marcos da Matta Guedes P12,
Peneau J13,
Marcet P14,
Padilla C15,
Cruz-Robles D16,
Valencia E17,
Crisante GE18,
Greif G19,
Zulantay I20,
Costales JA21,
Alvarez-Martínez M22,
Martínez NE23,
Villarroel R24,
Villarroel S25,
Sánchez Z26,
Bisio M1,
Parrado R25,
Maria da Cunha Galvão L12,
Jácome da Câmara AC12,
Espinoza B10,
Alarcón de Noya B9,
Puerta C7,
Riarte A4,
Diosque P6,
Sosa-Estani S4,
Guhl F5,
Ribeiro I27,
Aznar C13,
Britto C2,
Yadón ZE28,
Schijman AG29.
Abstract
An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and quantification of parasitic loads in peripheral blood samples from Chagas disease patients. Two methods were studied: Satellite DNA (SatDNA) qPCR and kinetoplastid DNA (kDNA) qPCR. Both methods included an internal amplification control. Reportable range, analytical sensitivity, limits of detection and quantification, and precision were estimated according to international guidelines. In addition, inclusivity and exclusivity were estimated with DNA from stocks representing the different Trypanosoma cruzi discrete typing units and Trypanosoma rangeli and Leishmania spp. Both methods were challenged against 156 blood samples provided by the participant laboratories, including samples from acute and chronic patients with varied clinical findings, infected by oral route or vectorial transmission. kDNA qPCR showed better analytical sensitivity than SatDNA qPCR with limits of detection of 0.23 and 0.70 parasite equivalents/mL, respectively. Analyses of clinical samples revealed a high concordance in terms of sensitivity and parasitic loads determined by both SatDNA and kDNA qPCRs. This effort is a major step toward international validation of qPCR methods for the quantification of T. cruzi DNA in human blood samples, aiming to provide an accurate surrogate biomarker for diagnosis and treatment monitoring for patients with Chagas disease. Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.
- PMID:
- 26320872
- [PubMed - in process]
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