Ahead of Print -Evidence for Elizabethkingia anophelis Transmission from Mother to Infant, Hong Kong - Volume 21, Number 2—February 2015 - Emerging Infectious Disease journal - CDC
Volume 21, Number 2—February 2015
Evidence for Elizabethkingia anophelis Transmission from Mother to Infant, Hong Kong
Microbial genome sequencing can enhance diagnosis and control of infectious diseases (1,2). Its ultimate molecular resolution is superior to other phenotypic and genotypic tests and enables not only rapid microbial identification but also characterization of transmission events. The technique has been applied in large-scale infectious disease outbreaks such as those caused by Escherichia coli O104:H4, Staphylococcus aureus,Streptococcus pyogenes, Enterococcus faecium, Pseudomonas aeruginosa, Vibrio cholerae, and mycobacteria (3–14). However, the routine application of this method in diagnostic microbiology and infection control, especially for less well-defined, emerging pathogens, is yet to be explored.
Elizabethkingia anophelis is a recently discovered bacterium isolated from the midgut of the Anopheles gambiaemosquito in 2011 (15). The genus Elizabethkingia also includes E. meningoseptica (previously namedChryseobacterium/Flavobacterium meningosepticum) and E. miricola (16). E. meningoseptica causes neonatal sepsis and infections in immunocompromised persons. E. anophelis has also recently been reported to cause neonatal meningitis in the Central African Republic, and a nosocomial outbreak was reported in an intensive care unit in Singapore (17–19). However, the role of mosquitoes or other sources in the transmission of E. anophelisremains unclear.
In 2012, we encountered 3 cases of Elizabethkingia sepsis associated with meningitis in 2 neonates and chorioamnionitis in a neonate’s mother in a hospital in Hong Kong. Three strains of Elizabethkingia-like, gram-negative bacilli sharing similar phenotypic characteristics were isolated from the 3 patients, but confident identification results were not obtained by matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry and 16S rRNA gene sequencing. Moreover, clinical and microbiological data did not provide adequate clues about the possible transmission route. We therefore attempted to use draft genome sequencing to rapidly dissect transmission pathways and confirm the identity of the species.
Figure 1. Clinical course of illness in 3 patients infected with Elizabethkingia anophelisin whom sepsis developed and the mother of patient 1, who had culture-negative postpartum fever, Hong Kong, 2012. Locations where...
Dr. Lau is a clinical professor in the Department of Microbiology at The University of Hong Kong. Her research focuses on microbial genomics for studying emerging infectious diseases.
We thank Cheung-Hing Foo for technical support in bacterial identification and members of the Centre for Genomic Sciences, The University of Hong Kong, for their technical support in genome sequencing.
This work was supported by Health and Medical Research Fund, Food and Health Bureau, The Government of the Hong Kong Special Administrative Region; Strategic Research Theme Fund, Committee for Research and Conference Grant, and University Development Fund, The University of Hong Kong; the Shaw Foundation; donation from Ms. Eunice Lam; and Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.
- Loman NJ, Constantinidou C, Chan JZ, Halachev M, Sergeant M, Penn CW, High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity. Nat Rev Microbiol. 2012;10:599–606 . DOIPubMed
- Fournier PE, Drancourt M, Raoult D. Bacterial genome sequencing and its use in infectious diseases. Lancet Infect Dis. 2007;7:711–23 .DOIPubMed
- Shah MA, Mutreja A, Thomson N, Baker S, Parkhill J, Dougan G, Genomic epidemiology of Vibrio cholerae O1 associated with floods, Pakistan, 2010.Emerg Infect Dis. 2014;20:13–20 . DOIPubMed
- Snyder LA, Loman NJ, Faraj LA, Levi K, Weinstock G, Boswell TC, Epidemiological investigation of Pseudomonas aeruginosa isolates from a six-year-long hospital outbreak using high-throughput whole genome sequencing. Euro Surveill. 2013;18:20611 .PubMed
- Harris SR, Cartwright EJ, Török ME, Holden MT, Brown NM, Ogilvy-Stuart AL, Whole-genome sequencing for analysis of an outbreak of methicillin-resistant Staphylococcus aureus: a descriptive study. Lancet Infect Dis. 2013;13:130–6 . DOIPubMed
- Walker TM, Ip CL, Harrell RH, Evans JT, Kapatai G, Dedicoat MJ, Whole-genome sequencing to delineate Mycobacterium tuberculosis outbreaks: a retrospective observational study. Lancet Infect Dis. 2013;13:137–46. DOIPubMed
- Bryant JM, Grogono DM, Greaves D, Foweraker J, Roddick I, Inns T, Whole-genome sequencing to identify transmission of Mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study. Lancet. 2013;381:1551–60. DOIPubMed
- Tse H, Bao JY, Davies MR, Maamary P, Tsoi HW, Tong AH, Molecular characterization of the 2011 Hong Kong scarlet fever outbreak. J Infect Dis.2012;206:341–51. DOIPubMed
- Brown CC, Olsen RJ, Fittipaldi N, Morman ML, Fort PL, Neuwirth R, Spread of virulent group A Streptococcus type emm59 from Montana to Wyoming, USA. Emerg Infect Dis. 2014;20:679–81 . DOIPubMed
- Köser CU, Holden MT, Ellington MJ, Cartwright EJ, Brown NM, Ogilvy-Stuart AL, Rapid whole-genome sequencing for investigation of a neonatal MRSA outbreak. N Engl J Med. 2012;366:2267–75 . DOIPubMed
- Loman NJ, Constantinidou C, Christner M, Rohde H, Chan JZ, Quick J, A culture-independent sequence-based metagenomics approach to the investigation of an outbreak of Shiga-toxigenic Escherichia coli O104:H4. JAMA. 2013;309:1502–10. DOIPubMed
- Price JR, Golubchik T, Cole K, Wilson DJ, Crook DW, Thwaites GE, Whole-genome sequencing shows that patient-to-patient transmission rarely accounts for acquisition of Staphylococcus aureus in an intensive care unit. Clin Infect Dis. 2014;58:609–18. DOIPubMed
- Pérez-Lago L, Comas I, Navarro Y, González-Candelas F, Herranz M, Bouza E, Whole genome sequencing analysis of intrapatient microevolution inMycobacterium tuberculosis: potential impact on the inference of tuberculosis transmission. J Infect Dis. 2014;209:98–108 . DOIPubMed
- Johnson PD, Ballard SA, Grabsch EA, Stinear TP, Seemann T, Young HL, A sustained hospital outbreak of vancomycin-resistant Enterococcus faecium bacteremia due to emergence of vanB E. faecium sequence type 203. J Infect Dis. 2010;202:1278–86 . DOIPubMed
- Kämpfer P, Matthews H, Glaeser SP, Martin K, Lodders N, Faye I. Elizabethkingia anophelis sp. nov., isolated from the midgut of the mosquitoAnopheles gambiae. Int J Syst Evol Microbiol. 2011;61:2670–5. DOIPubMed
- Kim KK, Kim MK, Lim JH, Park HY, Lee ST. Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol. 2005;55:1287–93.DOIPubMed
- Frank T, Gody JC, Nguyen LB, Berthet N, Le Fleche-Mateos A, Bata P, First case of Elizabethkingia anophelis meningitis in the Central African Republic. Lancet. 2013;381:1876. DOIPubMed
- Bobossi-Serengbe G, Gody JC, Beyam NE, Bercion R. First documented case of Chryseobacterium meningosepticum meningitis in Central African Republic. Med Trop (Mars). 2006;66:182–4 .PubMed
- Teo J, Tan SY, Tay M, Ding Y, Kjelleberg S, Givskov M, First case of E anophelis outbreak in an intensive-care unit. Lancet. 2013;382:855–6.DOIPubMed
- Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests. Approved standard, 11th ed. M02–A11. Wayne (PA): The Institute; 2012.
- Lau SK, Tang BS, Curreem SO, Chan TM, Martelli P, Tse CW, Matrix-assisted laser desorption ionization–time of flight mass spectrometry for rapid identification of Burkholderia pseudomallei: importance of expanding databases with pathogens endemic to different localities. J Clin Microbiol.2012;50:3142–3. DOIPubMed
- Woo PC, Lau SK, Teng JL, Que TL, Yung RW, Luk WK, L Hongkongensis study group. Association of Laribacter hongkongensis in community-acquired gastroenteritis with travel and eating fish: a multicentre case-control study. Lancet. 2004;363:1941–7. DOIPubMed
- Lau SK, Curreem SO, Lin CC, Fung AM, Yuen KY, Woo PC. Streptococcus hongkongensis sp. nov., isolated from a patient with an infected puncture wound and from a marine flatfish. Int J Syst Evol Microbiol. 2013;63:2570–6. DOIPubMed
- Tse H, Tsoi HW, Leung SP, Lau SK, Woo PC, Yuen KY. Complete genome sequence of Staphylococcus lugdunensis strain HKU09–01. J Bacteriol.2010;192:1471–2. DOIPubMed
- Woo PC, Lau SK, Tse H, Teng JL, Curreem SO, Tsang AK, The complete genome and proteome of Laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats. PLoS Genet. 2009;5:e1000416. DOIPubMed
- Delcher AL, Bratke KA, Powers EC, Salzberg SL. Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics. 2007;23:673–9.DOIPubMed
- Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, The RAST server: rapid annotations using subsystems technology. BMC Genomics.2008;9:75. DOIPubMed
- Liu B, Pop M. ARDB—antibiotic resistance genes database. Nucleic Acids Res. 2009;37:D443–7 . DOIPubMed
- Auch AF, Klenk HP, Göker M. Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs.Stand Genomic Sci. 2010;2:142–8. DOIPubMed
- Dussurget O. New insights into determinants of Listeria monocytogenes virulence. Int Rev Cell Mol Biol. 2008;270:1–38.
- Kreft J, Vázquez-Boland JA, Altrock S, Dominguez-Bernal G, Goebel W. Pathogenicity islands and other virulence elements in Listeria. Curr Top Microbiol Immunol. 2002;264:109–25 .PubMed
- Hoffman JA, Badger JL, Zhang Y, Huang SH, Kim KS. Escherichia coli K1 aslA contributes to invasion of brain microvascular endothelial cells in vitro and in vivo. Infect Immun. 2000;68:5062–7. DOIPubMed
- Cheng C, Chen J, Fang C, Xia Y, Shan Y, Liu Y, Listeria monocytogenes aguA1, but not aguA2, encodes a functional agmatine deiminase: biochemical characterization of its catalytic properties and roles in acid tolerance. J Biol Chem. 2013;288:26606–15. DOIPubMed
- Matyi SA, Hoyt PR, Hosoyama A, Yamazoe A, Fujita N, Gustafson JE. Draft genome sequences of Elizabethkingia meningoseptica. Genome Announc.2013;1:e00444–13.
- Kukutla P, Lindberg BG, Pei D, Rayl M, Yu W, Steritz M, Draft genome sequences of Elizabethkingia anophelis strains R26T and Ag1 from the midgut of the malaria mosquito Anopheles gambiae. Genome Announc. 2013;1:e01030–13.
- Balm MN, Salmon S, Jureen R, Teo C, Mahdi R, Seetoh T, Bad design, bad practices, bad bugs: frustrations in controlling an outbreak ofElizabethkingia meningoseptica in intensive care units. J Hosp Infect. 2013;85:134–40. DOIPubMed
- Quick J, Constantinidou C, Pallen MJ, Oppenheim B, Loman NJ. Draft genome sequence of Elizabethkingia meningoseptica isolated from a traumatic wound. Genome Announc. 2014;2:e00355–14.
- Sarma S, Kumar N, Jha A, Baveja U, Sharma S. Elizabethkingia meningosepticum: an emerging cause of septicemia in critically ill patients. J Lab Physicians. 2011;3:62–3. DOIPubMed
- Teo J, Tan SY, Liu Y, Tay M, Ding Y, Li Y, Comparative genomic analysis of malaria mosquito vector-associated novel pathogen Elizabethkingia anophelis. Genome Biol Evol. 2014;6:1158–65. DOIPubMed
- Holden MT, Feil EJ, Lindsay JA, Peacock SJ, Day NP, Enright MC, Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance. Proc Natl Acad Sci U S A. 2004;101:9786–91. DOIPubMed
Suggested citation for this article: Lau SKP, Wu AKL, Teng JLL, Tse H, Curreem SOT, Tsui SKW, et al. Evidence for Elizabethkingia anophelis transmission from mother to infant, Hong Kong. Emerg Infect Dis [Internet]. 2015 Feb [date cited]. http://dx.doi.org/10.3201/eid2102.140623
1These authors contributed equally to this article.
No hay comentarios:
Publicar un comentario