Protocol for Metagenomic Virus Detection in Clinical Specimens1 - Volume 21, Number 1—January 2015 - Emerging Infectious Disease journal - CDC
Volume 21, Number 1—January 2015
Protocol for Metagenomic Virus Detection in Clinical Specimens1
Viruses responsible for disease outbreaks in humans naturally emerge either from the human population or as zoonoses by transmission from animal hosts (1). Viruses can also emerge unnaturally, either directly (e.g., bioterrorist attacks) or accidentally (e.g., laboratory infections). Despite these possibilities of virus emergence, 60% of emerging viruses have a zoonotic origin, thus highlighting transmission from animals to humans as a major threat to public health (2). Whenever viruses emerge, prompt identification of the agent and implementation of control measures to contain the outbreak are required.
Currently, various next-generation sequencing (NGS) approaches provide solutions for detection of purified and concentrated viruses (i.e., from cell culture). However, for clinical specimens, such as blood, other fluids, or infected organ tissues, successful detection of viruses is less likely because virus-to-host genome ratios are insufficient (3–6). Use of tissues from persons with suspected infections for virus detection enables elucidation of infection directly at the site of viral replication. Although detecting viruses directly from infected organ tissue provides obvious and valuable advantages, reliable purification of viruses directly from tissues still remains a challenge.
In this study, we quantifiably and extensively compared classical and modern experimental approaches for virus purification and enrichment to finalize a protocol for unbiased detection of emerging viruses directly from organ tissues (tissue-based unbiased virus detection for viral metagenomics [TUViD-VM]) for an increased signal-to-noise ratio (ratio of virus genome to host genome) in virus detection. Use of this approach will reduce the amount of host nucleic acids required and save money and time in preparation of samples for NGS and the subsequent bioinformatic analysis.
Dr. Kohl is a scientist at the Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany. Her research interests are the detection of emerging and reemerging viruses and the characterization of novel pathogens.
We thank Ute Kramer for providing technical assistance, Brunhilde Schweiger for providing influenza A virus strain PR8/38, Marc Hoferer for providing infected marmoset tissues, and Ina Smith and Ursula Erikli for copyediting this article.
This study was partially supported by a scholarship from the Konrad-Adenauer-Foundation (to C.K).
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Suggested citation for this article: Kohl C, Brinkmann A, Dabrowski PW, Radonić A, Nitsche A, Kurth A. Protocol for metagenomic virus detection in clinical specimens. Emerg Infect Dis [Internet]. 2015 Jan [date cited]. http://dx.doi.org/10.3201/eid2101.140766
1Preliminary results of this study were presented at the Biodefense and Emerging Infectious Diseases Meeting, January 29, 2014, Washington DC, USA.