lunes, 17 de junio de 2013

The genome sequence of Lone Star virus, a highly di... [PLoS One. 2013] - PubMed - NCBI

The genome sequence of Lone Star virus, a highly di... [PLoS One. 2013] - PubMed - NCBI

PLoS One. 2013 Apr 29;8(4):e62083. doi: 10.1371/journal.pone.0062083. Print 2013.

The genome sequence of Lone Star virus, a highly divergent bunyavirus found in the Amblyomma americanum tick.


Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, United States of America.


Viruses in the family Bunyaviridae infect a wide range of plant, insect, and animal hosts. Tick-borne bunyaviruses in the Phlebovirus genus, including Severe Fever with Thrombocytopenia Syndrome virus (SFTSV) in China, Heartland virus (HRTV) in the United States, and Bhanja virus in Eurasia and Africa have been associated with acute febrile illness in humans. Here we sought to characterize the growth characteristics and genome of Lone Star virus (LSV), an unclassified bunyavirus originally isolated from the lone star tick Amblyomma americanum. LSV was able to infect both human (HeLa) and monkey (Vero) cells. Cytopathic effects were seen within 72 h in both cell lines; vacuolization was observed in infected Vero, but not HeLa, cells. Viral culture supernatants were examined by unbiased deep sequencing and analysis using an in-house developed rapid computational pipeline for viral discovery, which definitively identified LSV as a phlebovirus. De novo assembly of the full genome revealed that LSV is highly divergent, sharing <61 a.="" a="" acid="" also="" americanum="" amino="" analysis="" and="" any="" arbovirus="" are="" as="" be="" bhanja="" bunyavirus.="" by="" clade="" clinical="" closely="" competence="" critical="" deep="" despite="" determine="" developing="" diagnostic="" disease="" diversity="" expanding="" first="" found="" genome="" genomes="" geographic="" given="" group="" growing="" health="" identifying="" identity="" importance="" in="" includes="" is="" its="" lsv="" members="" most="" of="" other="" overall="" p="" part="" phylogenetic="" potential="" power="" public="" range="" rapidly="" related="" risk="" sequence="" sequencing="" sfstv="" significance.="" step="" study="" that="" the="" this="" tick="" to="" tools="" transmission="" underscores="" vector.="" viruses="" was="" well-supported="" which="" with="">


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