domingo, 30 de junio de 2013

Undetected Multidrug-Resistant Tuberculosis Amplified by First-line Therapy in Mixed Infection - Vol. 19 No. 7 - July 2013 - Emerging Infectious Disease journal - CDC

Undetected Multidrug-Resistant Tuberculosis Amplified by First-line Therapy in Mixed Infection - Vol. 19 No. 7 - July 2013 - Emerging Infectious Disease journal - CDC

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Polyxeni PotterComments to Author
Author affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Suggested citation for this article
Charles E. Burchfield (1893–1967) The Insect Chorus (1917) Opaque and transparent watercolor with ink, graphite, and crayon on off-white paper (50.8 cm × 38.1 cm) Munson-Williams-Proctor Arts Institute, Museum of Art, Utica, New York, Edward W. Root Bequest, 1957

Volume 19, Number 7—July 2013


Undetected Multidrug-Resistant Tuberculosis Amplified by First-line Therapy in Mixed Infection

Suzanne M. Hingley-Wilson1Comments to Author , Rosalyn Casey1, David Connell, Samuel Bremang, Jason T. Evans, Peter M. Hawkey, Grace E. Smith, Annette Jepson, Stuart Philip, Onn Min Kon, and Ajit Lalvani
Author affiliations: Imperial College London, London, UK (S.M. Hingley-Wilson, R. Casey, D. Connell, S. Bremang, A. Lalvani); Heart of England National Health Service Foundation Trust, Birmingham, UK (J.T. Evans, P.M. Hawkey, G.E. Smith); School of Infection and Immunity, University of Birmingham, Birmingham (P.M. Hawkey); Imperial College National Health Service HealthcareTrust, London (A. Jepson, S. Philip, O.M. Kon); 1Current affiliation: University of Surrey, Guildford, UK.
Suggested citation for this article


Infections with >1 Mycobacterium tuberculosis strain(s) are underrecognized. We show, in vitro and in vivo, how first-line treatment conferred a competitive growth advantage to amplify a multidrug-resistant M. tuberculosis strain in a patient with mixed infection. Diagnostic techniques that identify mixed tubercle bacilli populations are needed to curb the spread of multidrug resistance.
As the number of multidrug-resistant tuberculosis (TB) cases continues to rise, so does the amplification of multidrug-resistant Mycobacterium tuberculosis strains during treatment (1,2). This amplification is generally assumed to result from in vivo evolution of drug resistance caused by poor therapy compliance or, in high-incidence settings, from exogenous reinfection with a multidrug-resistant strain. We report a case in which emergence of multidrug resistance did not result from in vivo acquisition of drug resistance by a drug-sensitive strain or from exogenous reinfection with an already resistant strain. By integrating epidemiologic, microbiological, and molecular strain typing data with in vitro competitive growth experiments, we provide evidence for an initial mixed infection with a drug-sensitive strain and an undetected drug-resistant strain that outgrew the sensitive strain under the selection pressure of first-line chemotherapy.
M. tuberculosis strains in sputum from TB-infected patients or in samples from the disease site are generally identified by strain typing a single broth culture or colony grown on solid medium. However, this method does not enable identification of mixed infections, and any treatment regimen would be determined on the basis of the drug sensitivity of the strain with the fastest growth rate in the in vitro culture. Use of suboptimal drug combinations could lead to selection of a slower growing, drug-resistant strain already present in the host and thus to treatment failure.
Studies of artificially mixed M. tuberculosis strains before and after culture showed that culturing can reduce the clonal complexity of the strains and that, in most samples (6/10), only 1 strain will be identified in mixed infections after culture (3). This suggests that mixed infections and clonal complexity are underrepresented in culture-based diagnoses of TB. In support of this suggestion, the results of molecular-based methods that use strain-specific PCR showed that 2.1%–19.0% of patients with active TB in moderate to high incidence countries were simultaneously infected with >2 strains (1,2,410).
Possible co-infection of patients with drug-sensitive and drug-resistant M. tuberculosis strains has been described (1,2), and modeling of the effect of such co-infection on the long-term dynamics of tuberculous infection has led to the hypothesis that persons with this type of infection may retain small populations of drug-resistant bacteria that can flourish after the host receives treatment (11). van Rie et al. showed the amplification of a drug-resistant strain after treatment and postulated selection of drug-resistant strains from an initial mixed infection through antimicrobial drug pressure (2). We confirm this hypothesis by combining detailed longitudinal clinical and microbiological observation with the use of novel in vitro growth competition assays to study 2 co-infecting patient strains in the presence and absence of the primary drug used in treatment.

The Study

The 2 M. tuberculosis strains were isolated from a 68-year-old man from Portugal. He did not have HIV and was treated as a confined inpatient, limiting the possibility that this was not a true in vivo mixed infection. Using a novel approach, we correlated in vitro growth and treatment characteristics for the patient strains with the in vivo strain predominance and persistence of a less-fit, drug-resistant strain. All samples were obtained with approval from St. Mary Hospital’s (London, UK) Research Ethics Center (no. 07/H0712/85) and with the patient’s written informed consent.
Details of the patient samples are in the Table. The initial bronchoalveolar lavage smear sample was positive for acid-fast bacilli (AFB); culture results were positive for fully sensitive M. tuberculosis. Treatment with isoniazid, rifampin, ethambutol, and pyrazinamide was begun. Because of the patient’s alcohol use, his treatment was managed on an inpatient basis in a single-patient, negative-pressure room. Two months later, repeat sputum smears were positive for AFB, and culture results were positive for fully sensitive M. tuberculosis. After 4 months of treatment, the patient’s clinical signs had not improved, and his sputum smear was still positive for AFB. Culture results for the sputum sample were positive for M. tuberculosis resistant to isoniazid and ethambutol; a modified treatment regime resolved the infection, and the patient was released the following month, by which time his smear and culture results were negative.

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