Coccidioides posadasii Infection in Bats, Brazil - Vol. 18 No. 4 - April 2012 - Emerging Infectious Disease journal - CDC
Table of Contents
Volume 18, Number 4–April 2012
Volume 18, Number 4—April 2012
Coccidioides posadasii Infection in Bats, Brazil
AbstractTo analyze the eco-epidemiologic aspects of Histoplasma capsulatum in Brazil, we tested 83 bats for this fungus. Although H. capsulatum was not isolated, Coccidioides posadasii was recovered from Carollia perspicillata bat lungs. Immunologic studies detected coccidioidal antibodies and antigens in Glossophaga soricina and Desmodus rotundus bats.
To analyze the eco-epidemiologic aspects of H. capsulatum in northeast Brazil, we captured bats from urban and rural areas of Ceará State. However, the research revealed the existence of a bat that was naturally infected with Coccidioides posadasii and 2 other chiropterans with coccidioidal immunologic responses. This fungal pathogen can cause coccidioidomycosis, a serious infection in humans and animals. The mycosis is presently considered to be endemic to Northeast Brazil, as evidenced by human autochthonous cases (6–8), positive coccidioidin skin-test results (7), and isolation of the fungus from soil (7,9). We describe the isolation of C. posadasii in bats and discuss the epidemiologic effects of this finding.
Immediately after capture, the bats were euthanized by an overdose of diethyl ether by inhalation, and their spleen, liver, and lungs were analyzed for H. capsulatum isolation. Fragments of each organ were homogenized by maceration in saline supplemented with 200 mg/L chloramphenicol. Aliquots of 100 µL were seeded onto plates containing brain–heart infusion agar, supplemented with 1% glucose, 0.1% l-cysteine, 200 mg/L chloramphenicol, and 0.05% cycloheximide, and incubated at 25°C or 35°C for as long as 6 weeks (10). Remaining aliquots of each homogenate, as well as organ fragments, were kept at −20°C.