CIENCIASMEDICASNEWS: Medical News | Medical Articles: Tips for Successful Immunofluorescence in Cultured Cells

lunes, 22 de julio de 2019

Medical News | Medical Articles: Tips for Successful Immunofluorescence in Cultured Cells

	July 19, 2019
	Fluorescence
	The latest fluorescence news from NewsMedical

Tips for Successful Immunofluorescence in Cultured Cells

Immunostaining is a complex technology and subject to several technical obstacles, which could mean that after a lot of time and effort the result is either no signal or one which is impossible to interpret, instead of a clear and well defined set of images. But even this is not the worst scenario.

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			Understanding Fluorochromes for Use in Flow Cytometry Flow cytometry is a popular cell biology laboratory method. It uses a laser for rapid analysis, quantification, and sorting of a suspension of live cells. In a matter of nanoseconds, profiles of the cell population’s properties are acquired, including size and granulation. This is based on the characteristic pattern of light refracted from the cells.

			Plasmon-enhanced Fluorescence Spectroscopy Plasmon-enhanced fluorescence spectroscopy is a useful tool for imaging cells and other samples on a single-molecule level. This method poses several advantages over conventional fluorescence microscopy.

	Scientists develop universal FACS-based approach to heterogenous cell sorting, propelling organoid research A team at the Icahn School of Medicine at Mount Sinai have developed a series of novel fluorescence‐assisted cell sorting (FACS) protocols that enable isolation of neural progenitors, astrocytes, and neurons from virus‐infected cerebral organoids, for downstream multi-omics analysis.

	Flow Cytometry Techniques used in Medicine and Research Fluorescence-activated cell sorting (FACS) and flow cytometry are often used as interchangeable terms. However, FACS is a specialized method of flow cytometry that helps to physically sort a cell mixture into different populations.

	Advances in Fluorescence Microscopy In fluorescence microscopy, a specimen emits energy when activated by light of a certain wavelength. Some substances such as chlorophyll and some minerals will do this naturally, while others will need the help of other chemical triggers.