Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool. - PubMed - NCBI

Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool. - PubMed - NCBI

Emerg Infect Dis. 2016 Feb;22(2). doi: 10.3201/eid2202.151796.

Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool.

Hoenen T, Groseth A, Rosenke K, Fischer RJ, Hoenen A, Judson SD, Martellaro C, Falzarano D, Marzi A, Squires RB, Wollenberg KR, de Wit E, Prescott J,Safronetz D, van Doremalen N, Bushmaker T, Feldmann F, McNally K, Bolay FK, Fields B, Sealy T, Rayfield M, Nichol ST, Zoon KC, Massaquoi M, Munster VJ,Feldmann H.

Abstract

Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.

KEYWORDS:

DNA; Ebola hemorrhagic fever; Ebola virus; Ebola virus disease; Ebolavirus; Liberia; West Africa; disease outbreaks; high-throughput nucleotide sequencing; molecular diagnostic techniques; nanopore sequencing; viruses

PMID:

 

26812583

 

[PubMed - in process] 

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